Comprehensive characterization of multiple components ofZiziphus jujuba MillusingUHPLC‐Q‐ExactiveOrbitrap Mass Spectrometers

Abstract

Ziziphus jujuba Mill is the dried ripe fruit of the Rhamnaceae family; it is widely distributed in Shandong, Henan, Liaoning, and other places in China. In folk medicine, it was used to restore vital energy, as a blood tonic, and for the treatment of spleen deficiency. To date, a complete investigation of the compounds of Z. jujuba has rarely been performed. Therefore, a reliable strategy based on UHPLC‐Q‐Exactive Orbitrap MS, combined with trace data acquisition mode (parallel reaction monitoring scanning, PRM) and multiple data processing methods, is necessary for the characterization of compounds in the Z. jujuba. Ultimately, 295 compounds, including 69 flavonoids, 60 alkaloids, 82 phenylpropanoids, 52 organic acids, and 32 other components, were identified in the Z. jujuba; of these, 270 have been reported in Z. jujuba for the first time. This study provides deep insights into the chemistry of Z. jujuba and could be useful for further studies aimed at identifying the factors contributing to the health benefits attributed to this fruit.

Qualitative Analysis of Multiple Phytochemical Compounds in Tojapride Based on UHPLC Q-Exactive Orbitrap Mass Spectrometry

“…Then, they were tentatively characterized as quercetin-O-glucoside-rutinoside. Similarly, compounds 94 and 99 were characterized as methyl-quercetin-O-rutinoside [ 5 ].…”

“…However, the characteristic fragment ions at m / z 151.0028 and 119.0492 indicated that they possessed a naringenin group. Therefore, they were tentatively identified as being naringenin 7-O-glucoside isomers [ 5 ]. Likewise, compounds 22 , 26 , 40 , 47 , and 72 were tentatively proposed as being naringenin 4`-O-glucoside 7-O-rutinoside isomers, and compounds 65 , 76 , and 113 were tentatively identified as being narirutin isomers [ 14 ].…”

“…Compound 31 was detected at 11.08 min, possessing the quasi-molecular ions [M+H] + at m / z 505.2306 and MS/MS fragment ion at m / z 342.1698, due to the neutral loss of glucose reside (162 Da), and this indicated the presence of magnoflorine. Thus, it was identified as magnoflorine-O-glucoside [ 5 ].…”

“…TCMP has attracted considerable attention in great many fields due to its effective therapeutic performance and low toxicity [ 3 , 4 ]. With the development of analytical techniques, the combination of ultra-high-performance liquid chromatography and mass spectrometry (UHPLC/MS) has had a significant impact on TCMP development in recent years [ 5 , 6 ]. They feature powerful analytical figures of merit (sensitivity, selectivity, speed of analysis) and have a wide scope of application in the qualitative and quantitative analysis of the TCMP [ 7 ].…”

Comparative prototypes and metabolites of Du-zhi pill in normal and cerebral ischemia rats by UHPLC-Q-TOF-MS/MS method

“…It is necessary to compare the metabolism of DZP in normal and cerebral ischemia model rats in order to gain a better understanding of DZP metabolism in these rats. In recent years, high-resolution mass spectrometry has become a powerful and reliable analytical technique for metabolite identification due to its excellent accurate mass measurement, sensitivity, and high resolution [ [15] , [16] , [17] ]. In this study, ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to investigate the metabolic profile of DZP in normal and middle cerebral artery occlusion (MCAO) model rat brain samples and compare the differences between normal and cerebral ischemia pathological state.…”