Comprehensive circular RNA profiling reveals circ_0002060 as a potential diagnostic biomarkers for osteoporosis

Abstract

Osteoporosis (OP) is a multifactorial and common bone disease worldwide. The clinical diagnosis and treatment of osteoporosis has limitations since changes in bone density occur slightly. Thus, it is critically needed to look for new biomarkers to identify and cure osteoporosis. CircRNAs are important molecular regulators that participate in various biological processes and present tremendous therapeutic potential. The role of circRNAs in osteoporosis remains largely unknown. Here, we explore the expression of circRNAs in osteoporosis using microarray analysis, and identify potential circRNAs associated with osteoporosis by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) validation and statistical analysis in clinical samples. As a result, circ_0006873 and circ_0002060 were found to be significantly upregulated between nonosteoporotic controls and osteoporosis patients (n = 40, respectively). Pearson correlation analysis showed that the level of circ_0006873 and circ_0002060 were associated with the low‐bone mineral density in patients with osteoporosis. Furthermore, ROC analysis indicated that circ_0002060 showed potential diagnostic value for osteoporosis. Taken together results using bioinformatics, experimental analysis, and disease models, we revealed that circ_0002060 could be used as potential diagnostic biomarker and therapeutic target in osteoporosis

Circular RNAs: Promising Biomarkers for Age-related Diseases

“…One study reported that circRUNX2 can sponge miR-203 and promote the expression of osteogenic differentiation-related proteins, such as RUNX2, OCN, OPN, and BSP, consequently preventing osteoporosis [90]. Another study explored the expression of circRNAs using microarray analysis and identified circ_0002060 as a potential diagnostic biomarker and therapeutic target for osteoporosis [91].…”

CircFOXP1/FOXP1 promotes osteogenic differentiation in adipose‐derived mesenchymal stem cells and bone regeneration in osteoporosis via miR‐33a‐5p

“…OP is a progressive metabolic skeletal condition that increases the fragility fracture risk caused by low bone mass architectural deterioration of bone microstructure because of an imbalance between bone resorption and bone formation 35‐37 . To date, substantial investigations have reported that circRNAs take part in regulating different pathophysiological events, including tumorigenesis, organogenesis and tissue development, and function as biomarkers in various human processes, such as osteoporosis and cancer 38‐40 . In the present study, circRNA expression profiles were evaluated and 4972 circRNAs were significantly expressed in the OP group compared to the non‐OP group.…”

“…[35][36][37] To date, sub- cancer. [38][39][40] In the present study, circRNA expression profiles were in transduced hASCs cultured in PM or OM measured by qRT-PCR. G, Western blot analysis detected RUNX2 and OCN protein expression levels on day 7 in transduced hASCs cultured in PM or OM.…”

Comprehensive Analysis of Differentially Expressed Circular RNAs in Patients with Senile Osteoporotic Vertebral Compression Fracture

“…Their data identified 203 upregulated and 178 downregulated circRNAs and revealed circ_0001275 was a potential diagnostic biomarker according to qRT-PCR results. Huang et al [ 24 ] performed microarray analysis and PCR validation, and their result showed that circ_0006873 and circ_0002060 were linked to the low BMD state. In our study, rather than investigating by microarray which could identify only thousands of circRNAs, we chose to sequence all circRNAs that were attained in blood samples from patients with newly diagnosed senile OVCF and healthy controls.…”