2016
DOI: 10.3892/ijo.2016.3497
|View full text |Cite
|
Sign up to set email alerts
|

Comprehensive evaluation and validation of targeted next-generation sequencing performance in two clinical laboratories

Abstract: Abstract. Next-generation sequencing (NGS) has led to breakthroughs for genetic and genomic analyses and personalized medicine approaches for many diseases. More and more clinical laboratories are using NGS as a genetic screening tool for providing mutation information that is used to select the best treatment regimens for cancer patients. However, several obstacles prevent the routine implementation of NGS technology into the clinical molecular diagnosis setting: the sophisticated sample preparation process, … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

1
20
0

Year Published

2017
2017
2023
2023

Publication Types

Select...
5

Relationship

2
3

Authors

Journals

citations
Cited by 8 publications
(21 citation statements)
references
References 29 publications
1
20
0
Order By: Relevance
“…No. 969056) was used for generating sequencing data for testing and evaluating MGE and ELECTRO as previously described [22]. This control DNA contains more than 500 COSMIC mutations from 53 genes (Thermo Fisher, Waltham, MA; Cat.…”
Section: Methodsmentioning
confidence: 99%
See 4 more Smart Citations
“…No. 969056) was used for generating sequencing data for testing and evaluating MGE and ELECTRO as previously described [22]. This control DNA contains more than 500 COSMIC mutations from 53 genes (Thermo Fisher, Waltham, MA; Cat.…”
Section: Methodsmentioning
confidence: 99%
“…The sequencing cost has been significantly reduced from more than several thousands of dollars per exome to near one thousand dollars [21]. The size of sequencing data has also been dramatically reduced because of the increasing demand of a targeted sequencing focusing on 5–300 genes rather than exome sequencing [2225]. Exome sequencing strategy is still invaluable for identifying new biomarkers by big research consortiums such as TCGA [26,27].…”
Section: Introductionmentioning
confidence: 99%
See 3 more Smart Citations