Comprehensive Evaluation of Cryopreserved Bone‐Derived Osteoblasts for the Repair of Segmental Mandibular Defects in Canines

Wang, Shaoyi; Zhao, Jun; Zhang, Wenjie; Ye, Dongxia; Zhang, Xiaochen; Zou, Duohong; Zhang, Xiuli; Sun, Xiaojuan; Sun, Shuyang; Zhang, Weijie; Chen, Yang; Jiang, Xinquan; Zhang, Zhiyuan

doi:10.1111/cid.12164

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…Moreover, the osteogenic differentiation process lasted from the early to the late healing stages. These findings are consistent with the results reported in previous studies [ 39 ]. The mechanism underlying the failure of CSD to heal without intervention over the animal's lifetime is still unknown.…”

Section: Discussionsupporting

confidence: 94%

Determination of critical-sized defect of mandible in a rabbit model: Micro-computed tomography, and histological evaluation

Wang¹,

Zhang²,

Mei

et al. 2023

Heliyon

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Section: Discussionsupporting

confidence: 94%

Determination of critical-sized defect of mandible in a rabbit model: Micro-computed tomography, and histological evaluation

Wang¹,

Zhang²,

Mei

et al. 2023

Heliyon

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“…The CSD ranged from 4 mm in diameter (rats) to 15 mm in length (rabbits) in small animals, and from 6 mm in diameter (pigs) to 20 mm in length (dogs) in large animals. Four studies reported the use of ‘segmental’ defects in monkeys (15 mm; Chanchareonsook et al ., ), dogs (20–30 mm; Zhao et al ., ; Wang et al ., ) or sheep (35 mm; Schliephake et al ., ), where a portion of the mandibular body was resected by either disrupting or preserving mandibular continuity. When continuity was disrupted a titanium plate was fixed with screws on either end for stabilization.…”

Section: Resultsmentioning

confidence: 99%

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

Shanbhag

Pandis

Mustafa

et al. 2016

J Tissue Eng Regen Med

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Regeneration of large, 'critical-size' bone defects remains a clinical challenge. Bone tissue engineering (BTE) is emerging as a promising alternative to autogenous, allogeneic and biomaterial-based bone grafting. The objective of this systematic review was to answer the focused question: in animal models, do cell-based BTE strategies enhance regeneration in alveolar bone critical-size defects (CSDs), compared with grafting with only biomaterial scaffolds or autogenous bone? Following PRISMA guidelines, electronic databases were searched for controlled animal studies reporting maxillary or mandibular CSD and implantation of mesenchymal stem cells (MSCs) or osteoblasts (OBs) seeded on biomaterial scaffolds. A random effects meta-analysis was performed for the outcome histomorphometric new bone formation (%NBF). Thirty-six studies were included that reported on large- (monkeys, dogs, sheep, minipigs) and small-animal (rabbits, rats) models. On average, studies presented with an unclear-to-high risk of bias and short observation times. In most studies, MSCs or OBs were used in combination with alloplastic mineral-phase scaffolds. In five studies, cells were modified by ex vivo gene transfer of bone morphogenetic proteins (BMPs). The meta-analysis indicated statistically significant benefits in favour of: (1) cell-loaded vs. cell-free scaffolds [weighted mean difference (WMD) 15.59-49.15% and 8.60-13.85% NBF in large- and small-animal models, respectively]; and (2) BMP-gene-modified vs. unmodified cells (WMD 10.06-20.83% NBF in small-animal models). Results of cell-loaded scaffolds vs. autogenous bone were inconclusive. Overall, heterogeneity in the meta-analysis was high (I > 90%). In summary, alveolar bone regeneration is enhanced by addition of osteogenic cells to biomaterial scaffolds. The direction and estimates of treatment effect are useful to predict therapeutic efficacy and guide future clinical trials of BTE. Copyright © 2016 John Wiley & Sons, Ltd.

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“…The sequence fluorescent labeling was utilized to observe osteogenesis and mineralization process in detail, as previously reported. , Alizalin Red S (ARS, 30 mg/kg, Sigma–Aldrich, USA) was injected intraperitoneally into rabbits at 4 weeks after operation, and Calcein Green (20 mg/kg, Sigma-Aldrich, USA) was injected at 8 weeks in the same way.…”

Section: Methodsmentioning

confidence: 99%

A Biomimetic Zinc Alloy Scaffold Coated with Brushite for Enhanced Cranial Bone Regeneration

Qingcheng

Jia

et al. 2020

ACS Biomater. Sci. Eng.

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Bone tissue engineering is considered as a promising pathway for bone regeneration and defect reconstruction, in which scaffolds play an important role. Zn alloy, which is a biodegradable metal material that has advantages of metallic and biodegradable characteristics, has its special features, especially the ideal degradation rate and acceptable biocompatibility, which make it worthy to be further investigated for medical applications. In this study, new biodegradable porous Zn alloy scaffolds with Ca–P coating were attempted to repair cranial bone defect, and in vitro and in vivo assays were conducted to evaluate its biocompatibility, osteo-inductivity, and osteo-conductivity. The results indicated that coated Zn alloy possessed good biocompatibility, with no cytotoxicity. It could also promote osteogenic differentiation and calcium deposition of rabbit BMSCs in vitro, and new bone formation around the scaffold in vivo. The biodegradable porous Zn alloy scaffold with Ca–P coating is considered to be promising in cranial bone defect repair.

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Comprehensive Evaluation of Cryopreserved Bone‐Derived Osteoblasts for the Repair of Segmental Mandibular Defects in Canines

Cited by 8 publications

References 42 publications

Determination of critical-sized defect of mandible in a rabbit model: Micro-computed tomography, and histological evaluation

Determination of critical-sized defect of mandible in a rabbit model: Micro-computed tomography, and histological evaluation

Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis

A Biomimetic Zinc Alloy Scaffold Coated with Brushite for Enhanced Cranial Bone Regeneration

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