Comprehensive evaluation of direct injection mass spectrometry for the quantitative profiling of volatiles in food samples

Lebrón-Aguilar, Rosa; Soria, Ana C.; Quintanilla-López, Jesús Eduardo

doi:10.1098/rsta.2015.0375

Cited by 5 publications

(3 citation statements)

References 22 publications

(47 reference statements)

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“…Despite several limitations, the DIMS is widely used in modern metabolomics investigations [32][33][34]. Due to technological advancements (application of modern high-resolution mass spectrometers, data processing procedures, etc.)…”

Section: Discussionmentioning

confidence: 99%

Blood Plasma Metabolome Profiling at Different Stages of Renal Cell Carcinoma

Maslov

Trifonova

Lichtenberg

et al. 2022

Cancers

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Early diagnostics significantly improves the survival of patients with renal cell carcinoma (RCC), which is the prevailing type of adult kidney cancer. However, the absence of clinically obvious symptoms and effective screening strategies at the early stages result to disease progression and survival rate reducing. The study was focused on revealing of potential low molecular biomarkers for early-stage RCC. The untargeted direct injection mass spectrometry-based metabolite profiling of blood plasma samples from 51 non-cancer volunteers (control) and 78 patients with different RCC subtypes and stages (early stages of clear cell RCC (ccRCC), papillary RCC (pRCC), chromophobe RCC (chrRCC) and advanced stages of ccRCC) was performed. Comparative analysis of the blood plasma metabolites between the control and cancer groups provided the detection of metabolites associated with different tumor stages. The designed model based on the revealed metabolites demonstrated high diagnostic power and accuracy. Overall, using the metabolomics approach the study revealed the metabolites demonstrating a high value for design of plasma-based test to improve early ccRCC diagnosis.

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Section: Discussionmentioning

confidence: 99%

Blood Plasma Metabolome Profiling at Different Stages of Renal Cell Carcinoma

Maslov

Trifonova

Lichtenberg

et al. 2022

Cancers

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“…A promising variant of utilizing MS for the analysis of VOCs are direct injection mass spectrometers (DIMS). These promise the ability to identify and quantify VOCs without a prior separation and in some cases even without proper calibration [ 116 ]. Hereby more specialized and soft ionization techniques are utilized in combination with a high-resolution mass analyzer (HRMS).…”

Section: Fast/screening Methods For On-site/mobile Analysis Of Vocsmentioning

confidence: 99%

On-Site Detection of Volatile Organic Compounds (VOCs)

Epping

Koch

2023

Molecules

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Volatile organic compounds (VOCs) are of interest in many different fields. Among them are food and fragrance analysis, environmental and atmospheric research, industrial applications, security or medical and life science. In the past, the characterization of these compounds was mostly performed via sample collection and off-site analysis with gas chromatography coupled to mass spectrometry (GC-MS) as the gold standard. While powerful, this method also has several drawbacks such as being slow, expensive, and demanding on the user. For decades, intense research has been dedicated to find methods for fast VOC analysis on-site with time and spatial resolution. We present the working principles of the most important, utilized, and researched technologies for this purpose and highlight important publications from the last five years. In this overview, non-selective gas sensors, electronic noses, spectroscopic methods, miniaturized gas chromatography, ion mobility spectrometry and direct injection mass spectrometry are covered. The advantages and limitations of the different methods are compared. Finally, we give our outlook into the future progression of this field of research.

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“…The interest in the monitoring of this bioprocess is high due to (i) the vast number of variables that can affect AF performances, and (ii) the crucial relevant impact of AF on wine quality. Non-separative approaches based on direct injection mass spectrometry (DIMS) have recently emerged as an alternative for the high-throughput and cost-effective quantitative profiling of volatiles in food and beverages [3]. To the best of our knowledge, no study has explored the potential of DIMS techniques to assess online VOC variability in association with alcoholic fermentation in wine [4].…”

Section: Introductionmentioning

confidence: 99%

PTR-ToF-MS for the Online Monitoring of Alcoholic Fermentation in Wine: Assessment of VOCs Variability Associated with Different Combinations of Saccharomyces/Non-Saccharomyces as a Case-Study

et al. 2020

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The management of the alcoholic fermentation (AF) in wine is crucial to shaping product quality. Numerous variables (e.g., grape varieties, yeast species/strains, technological parameters) can affect the performances of this fermentative bioprocess. The fact that these variables are often interdependent, with a high degree of interaction, leads to a huge ‘oenological space’ associated with AF that scientists and professionals have explored to obtain the desired quality standards in wine and to promote innovation. This challenge explains the high interest in approaches tested to monitor this bioprocess including those using volatile organic compounds (VOCs) as target molecules. Among direct injection mass spectrometry approaches, no study has proposed an untargeted online investigation of the diversity of volatiles associated with the wine headspace. This communication proposed the first application of proton-transfer reaction-mass spectrometry coupled to a time-of-flight mass analyzer (PTR-ToF-MS) to follow the progress of AF and evaluate the impact of the different variables of wine quality. As a case study, the assessment of VOC variability associated with different combinations of Saccharomyces/non-Saccharomyces was selected. The different combinations of microbial resources in wine are among the main factors susceptible to influencing the content of VOCs associated with the wine headspaces. In particular, this investigation explored the effect of multiple combinations of two Saccharomyces strains and two non-Saccharomyces strains (belonging to the species Metschnikowia pulcherrima and Torulaspora delbrueckii) on the content of VOCs in wine, inoculated both in commercial grape juice and fresh grape must. The results demonstrated the possible exploitation of non-invasive PTR-ToF-MS monitoring to explore, using VOCs as biomarkers, (i) the huge number of variables influencing AF in wine, and (ii) applications of single/mixed starter cultures in wine. Reported preliminary findings underlined the presence of different behaviors on grape juice and on must, respectively, and confirmed differences among the single yeast strains ‘volatomes’. It was one of the first studies to include the simultaneous inoculation on two non-Saccharomyces species together with a S. cerevisiae strain in terms of VOC contribution. Among the other outcomes, evidence suggests that the addition of M. pulcherrima to the coupled S. cerevisiae/T. delbrueckii can modify the global release of volatiles as a function of the characteristics of the fermented matrix.

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Comprehensive evaluation of direct injection mass spectrometry for the quantitative profiling of volatiles in food samples

Cited by 5 publications

References 22 publications

Blood Plasma Metabolome Profiling at Different Stages of Renal Cell Carcinoma

Blood Plasma Metabolome Profiling at Different Stages of Renal Cell Carcinoma

On-Site Detection of Volatile Organic Compounds (VOCs)

PTR-ToF-MS for the Online Monitoring of Alcoholic Fermentation in Wine: Assessment of VOCs Variability Associated with Different Combinations of Saccharomyces/Non-Saccharomyces as a Case-Study

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