Comprehensive Evaluation of the Effect of Ultraviolet Stress on Functional Phytochemicals of Hulless Barley (Qingke) Grass in Different Growth Times at Vegetative Stage

Abstract

The present study was executed to analyze the functional phytochemicals of hulless barley grass grown under different intensities of ultraviolet stress. The wheat seedlings were imposed to 0.5, 1.0, 1.5, 2.0, and 2.5 h ultraviolet radiation and harvested in different times at vegetative stage. Specifically, the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, and chlorophyll were determined and antioxidants capacity was evaluated by OH• and 2,2’-azino-bis­(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging ability. A mathematical model (Technique for Order Preference by Similarity to Ideal Solution, TOPSIS) was also employed for the comprehensive evaluation of functional components of hulless barley grass at different growth stages. The results showed that the UV stress could efficiently improve/preserve the contents of total polyphenols, total flavonoids, total triterpenes, total polysaccharides, proanthocyanidins, chlorophyll a, chlorophyll b, and total chlorophyll, as well as the OH• and ABTS scavenging capacity. TOPSIS evaluation revealed that the highest phytochemical contents were yield on the 15th day under 1.0 h ultraviolet treatment.

Quantitative Assessment of Abiotic Stress on the Main Functional Phytochemicals and Antioxidant Capacity of Wheatgrass at Different Seedling Age

“…An obvious accumulation peak was noticed on D12 and D21 (based on MANOVA, p < 0.05), which shared a similar changing pattern with the previous study in UV-treated barley grass where the peak appeared on D15 and D21 ( 24 ). Specifically, except for UV40 and PEG 25% dose group, the TTC of other groups increased significantly on D12.…”

“…The TSPC of wheatgrass in the present study ranged from 0.52 ± 0.07 to 2.41 ± 0.11 mg GE/g FW ( Figure 1E ). Some previous studies showed that the TSPC decreased significantly with the ripening or seedling age ( 24 , 61 ). In this study, all the tested groups shared similar accumulation patterns of TSPC that decreased from D9 to D12 and then climbed up significantly ( p < 0.05).…”

“…The cultivation of the wheatgrass was executed based on the procedures proposed by the team ( 24 ). Briefly, 55 g of the thrice-washed seeds were sown evenly in a hydroponic tray sized 32.5 × 24.5 × 4.5 cm in four rectangular pieces, then were semi-immersed in deionized water for 24 h away from light to let malt.…”

Influence of Different Growth Conditions on Saponarin, Water-Soluble Vitamins, and Mineral Content of Barley Sprouts Cultivated in Chamber System

“…또한, 새싹보리는 polyphenols, policosanol, glucosamine, GABA, saponarin 등의 이차대사산물을 다량 함유하고 있으며 이들의 생리활성에 대한 연구는 활발히 이루 어지고 있다 (Meng et al, 2015;Kowalczewski et al, 2020;Song et al, 2020). 이와 같은 식물체내 이차대사산물은 식물의 생육환경 변화에 스스로를 방어하기 위해 합성이 되기 때문에 온도, 광, 수분 등의 외부 환경조건 조절에 의한 이차대사산물 함량 변화에 대한 연구가 활발히 진행되고 있다 (Nezval et al, 2017;Chun et al, 2019;Chung et al, 2019;Yoon et al, 2019;Jiang et al, 2020;Lee et al, 2020). 또한, 품종과 발아기간에 따른 새싹보리 내 이차대사산 물 함량 차이에 관한 연구결과도 보고되고 있다 (Lee et al, 2017).…”

Methyl Jasmonate Promotes the Growth of Rosa Roxburghii via Balanceing Pathways referring to Indoleacetic acid (IAA) and Triterpenes.

“…At present, many triterpenoids containing C-28 carboxyl groups have been developed and applied as biological pesticides (Tian et al, 2021). In terms of defense function, the synthesis of triterpenes can increase the tolerance of plants to external stress and alleviate the damage of UV-B to plants (Jiang et al, 2020). In addition to the defense function, triterpenoids also have good antibacterial activity.…”