Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

Sung, Dong Yul; Vierling, Elizabeth; Guy, Charles L.

doi:10.1104/pp.126.2.789

Cited by 442 publications

(403 citation statements)

References 48 publications

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“…PCC6803. This result was also in agreement with previous phylogenic analyses of HSP70 in yeast, plants, and other organisms [4,5,33,37], and suggested that TaHSC70 was a homolog of chloroplast HSP70s with prokaryotic origin.…”

Section: Discussionsupporting

confidence: 92%

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

et al. 2010

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Members of the family of 70-kD heat shock proteins (HSP70 s) play various stress-protective roles in plants. In this study, a wheat HSP70 gene was isolated from a suppression subtractive hybridization (SSH) cDNA library of wheat leaves infected by Puccinia striiformis f. sp. tritici. The gene, that was designated as TaHSC70, was predicted to encode a protein of 690 amino acids, with a molecular mass of 73.54 KDa and a pI of 5.01. Further analysis revealed the presence of a conserved signature that is characteristic for HSP70s and phylogenetic analysis demonstrated that TaHSC70 is a homolog of chloroplast HSP70s. TaHSC70 mRNA was present in leaves of both green and etiolated wheat seedlings and in stems and roots. The transcript level in roots was approximately threefold less than in leaves but light-dark treatment did not charge TaHSC70 expression. Following heat shock of wheat seedlings at 40°C, TaHSC70 expression increased in leaves of etiolated seedlings but remained stable at the same level in green seedlings. In addition, TaHSC70 was differentially expressed during an incompatible and compatible interaction with wheat-stripe rust, and there was a transient increase in expression upon treatment with methyl jasmonate (MeJA) treatment. Salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) treatments had no influence on TaHSC70 expression. These results suggest that TaHSC70 plays a role in stress-related responses, and in defense responses elicited by infection with stripe rust fungus and does so via a JA-dependent signal transduction pathway.

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Section: Discussionsupporting

confidence: 92%

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

et al. 2010

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“…Heat shock proteins perform a variety of functions in plants from protein folding to assembly, translocation, and degradation in cellular processes and can assist in the refolding of proteins under stress conditions. It has been demonstrated in expression profile studies that HSP70 genes are expressed in response to stresses such as heat, cold, drought, chemical, and other environmental stresses in Arabidopsis and spinach (Guy and Li 1998;Lin et al 2001;Sung et al 2001;Wang et al 2004). …”

Section: Analysis Of Differentially Expressed Genesmentioning

confidence: 99%

Transcriptional responses of tolerant and susceptible soybeans to soybean aphid (Aphis glycines Matsumura) herbivory

Prochaska

Donze-Reiner

Marchi-Werle

et al. 2015

Arthropod-Plant Interactions

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Prochaska, Travis J.; Donze-Reiner, Teresa; Marchi-Werle, L.; Palmer, N. A.; Hunt, Thomas E.; Sarath, Gautam; and Heng-Moss, Tiffany, "Transcriptional responses of tolerant and susceptible soybeans to soybean aphid (Aphis glycines Matsumura) herbivory" (2015). Abstract The soybean aphid, Aphis glycines Matsumura, was introduced in 2000 to North America and has become one of the most significant pests to soybean, Glycine max (L.) Merrill, production. Possible solutions to this problem are the use of resistant plants and the understanding of the genes involved in plant resistance. In this study, we sought to better understand the genes involved in the tolerance response of soybean plants to the soybean aphid, utilizing tolerant (KS4202) and susceptible (K-03-4686) plants. Studies were conducted under greenhouse conditions. Leaf samples of both tolerant and susceptible plants were collected at day 5 and day 15 after infestation and analyzed by sequencing-by-synthesis on an Illumina GA II X instrument. In the tolerant genotype, 3 and 36 genes were found to be differentially expressed in the infested plants compared to the control treatments at day 5 and day 15, respectively. A similar comparison in the susceptible genotype revealed 0 and 11 genes to be differentially expressed at day 5 and day 15, respectively. Predominately, genes related to plant defense, such as WRKY transcription factors, peroxidases, and cytochrome p450s, were upregulated in the tolerant genotype 15 days post-infestation by aphids. In contrast, none of these genes were similarly up-regulated in the susceptible plants, suggesting that consistent elevation of defense responses is important to plant tolerance. However, significant genotypic differences in global gene expression were also found when transcriptomes from control uninfested plants were compared at both day 5 and 15. qPCR validation of select genes confirmed our RNA-seq data. These comparisons indicate that potentially broader regulation of transcriptomes also contributes to the tolerance response and provides data that the tolerant genotype (KS4202) could be useful in soybean breeding programs trying to minimize production losses accruing from soybean aphid feeding.

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“…The HSP70 gene family in Arabidopsis (Arabidopsis thaliana) comprises 14 members, of which five are predicted to be cytosolic (Sung et al, 2001). Since plant RNA viruses replicate in the cytoplasm, we studied these five members for their induction by a range of compatible viruses.…”

Section: Induction Of Hsp70 In Arabidopsismentioning

confidence: 99%

Virus Induction of Heat Shock Protein 70 Reflects a General Response to Protein Accumulation in the Plant Cytosol

et al. 2005

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Different cytoplasmically replicating RNA viruses were shown to induce a specific subset of heat-inducible heat shock protein 70 (HSP70) genes in Arabidopsis (Arabidopsis thaliana). To identify the inducing principle, a promoter::reporter system was developed for the facile analysis of differentially responding Arabidopsis HSP70 genes, by infiltration into Nicotiana benthamiana leaves. Through transient expression of individual viral cistrons or through deletion analysis of a viral replicon, we were unable to identify a unique inducer of HSP70. However, there was a positive correlation between the translatability of the test construct and the differential induction of HSP70. Since these data implied a lack of specificity in the induction process, we also expressed a random series of cytosolically targeted Arabidopsis genes and showed that these also differentially induced HSP70. Through a comparison of different promoterTreporter constructs and through measurements of the steady-state levels of the individual proteins, it appeared that the HSP70 response reflected the ability of the cytosol to sense individual properties of particular proteins when expressed at high levels. This phenomenon is reminiscent of the unfolded protein response observed when the induced accumulation of proteins in the endoplasmic reticulum also induces a specific suite of chaperones.

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Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

Cited by 442 publications

References 48 publications

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Characterization of a wheat HSP70 gene and its expression in response to stripe rust infection and abiotic stresses

Transcriptional responses of tolerant and susceptible soybeans to soybean aphid (Aphis glycines Matsumura) herbivory

Virus Induction of Heat Shock Protein 70 Reflects a General Response to Protein Accumulation in the Plant Cytosol

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