Comprehensive gas chromatography–electron ionisation mass spectrometric analysis of fatty acids and sterols using sequential one‐pot silylation: quantification and isotopologue analysis

Kloos, Dick-Paul; Lingeman, H.; Bracher, Franz; Müller, Christoph; Mayboroda, Oleg A.; Deelder, André M.; Niessen, W.M.A.; Giera, Martin

doi:10.1002/rcm.6923

Cited by 33 publications

(20 citation statements)

References 28 publications

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“…Target mRNA levels were normalized to Rplp0 and Ppia mRNA levels. Primer sequences and PCR conditions can be found in supplementary Table I. gWAT, liver, and diet FA composition FA composition analysis of gWAT, liver, and diet was carried out as described recently by Kloos et al ( 24 ). Briefl y, triplicate samples were weighed of ‫ف‬ 10 mg diet or organ from niacintreated and control mice.…”

Section: Quantitative Pcrmentioning

confidence: 99%

Prolonged niacin treatment leads to increased adipose tissue PUFA synthesis and anti-inflammatory lipid and oxylipin plasma profile

Heemskerk

Dharuri

Berg

et al. 2014

Journal of Lipid Research

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This article is available online at http://www.jlr.org Supplementary key words drug therapy/hypolipidemic drugs • adipocytes • fatty acid/biosynthesis • omega-3 fatty acids • cytochrome P450 • polyunsaturated fatty acid Niacin (vitamin B3) treatment reduces CVD and atherosclerosis development ( 1 ). These benefi cial effects are mediated, in part, by lowering circulating levels of LDLcholesterol, VLDL-TG, and lipoprotein(a) ( 2 ), as well as by increasing HDL-cholesterol ( 3 ). In addition, prolonged niacin treatment also decreases plasma, adipose tissue, and vascular infl ammation ( 4, 5 ), which might contribute to reducing CVD. The induction of these benefi cial effects after prolonged niacin treatment is in striking contrast to the unwanted acute niacin effects.Acutely, niacin binds to the inhibitory hydroxycarboxylic acid receptor 2 (HCA 2 ) (previously known as GPR109A). In adipocytes, this leads to an inhibition of adipocyte lipolysis followed by an acute reduction of plasma nonesterifi ed fatty acid (NEFA) levels. Lowering NEFA levels causes metabolic stress ( 6, 7 ), which increases stress hormone levels ( 8-12 ) after niacin treatment. In the skin Langerhans cells and keratinocytes, acute niacin binding to the

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Section: Quantitative Pcrmentioning

confidence: 99%

Prolonged niacin treatment leads to increased adipose tissue PUFA synthesis and anti-inflammatory lipid and oxylipin plasma profile

Heemskerk

Dharuri

Berg

et al. 2014

Journal of Lipid Research

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“…The mixture was centrifuged at about 1500 rpm for 2 min to precipitate sodium oxalate, and an aliquot of supernatant was directly injected for GC analysis 15,16 .…”

Section: Preparation Of Fatty Acid Methyl Esters Famementioning

confidence: 99%

“…1 . The acidity, firstly determined for both oils by titration β-tocopherol 9 1 (-) 7 1 (0-1) 6) γ-tocopherol 575 4 (520) 8) 4 1 (-) 6) δ-tocopherol 57 2 (148) 8) -(-) 6) Total 816 (688) 8) 59 (12)(13)(14)(15)(16)(17)(18) …”

Section: Nmr Ngerprinting Of Seed Oilsmentioning

confidence: 99%

Chemical Characterization and Antioxidant Activity of Amazonian (Ecuador) Caryodendron orinocense Karst. and Bactris gasipaes Kunth Seed Oils

et al. 2014

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“…It is well known that MtBSTFA forming tert-butyldimethylsilyl derivatives give rise to more intense and information-rich fragment ions especially when using electron ionization (EI) [65,66]. Unfortunately, the derivatization efficiency of MtBSTFA is rather poor, particularly for secondary and tertiary alcohols, likely due to sterical hindrance potentially leading to partial derivatization [67,68].…”

Section: Sample Preparationmentioning

confidence: 99%

Analytical Pitfalls and Challenges in Clinical Metabolomics

et al. 2016

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Metabolomics-based strategies have become an integral part of modern clinical research, allowing for a better understanding of pathophysiological conditions and disease mechanisms, as well as providing innovative tools for more adequate diagnostic and prognosis approaches. Metabolomics is considered an essential tool in precision medicine, which aims for personalized prevention and tailor-made treatments. Nevertheless, multiple pitfalls may be encountered in clinical metabolomics during the entire workflow, hampering the quality of the data and, thus, the biological interpretation. This review describes the challenges underlying metabolomics-based experiments, discussing step by step the potential pitfalls of the analytical process, including study design, sample collection, storage, as well as preparation, chromatographic and electrophoretic separation, detection and data analysis. Moreover, it offers practical solutions and strategies to tackle these challenges, ensuring the generation of high-quality data.

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Comprehensive gas chromatography–electron ionisation mass spectrometric analysis of fatty acids and sterols using sequential one‐pot silylation: quantification and isotopologue analysis

Cited by 33 publications

References 28 publications

Prolonged niacin treatment leads to increased adipose tissue PUFA synthesis and anti-inflammatory lipid and oxylipin plasma profile

Prolonged niacin treatment leads to increased adipose tissue PUFA synthesis and anti-inflammatory lipid and oxylipin plasma profile

Chemical Characterization and Antioxidant Activity of Amazonian (Ecuador) Caryodendron orinocense Karst. and Bactris gasipaes Kunth Seed Oils

Analytical Pitfalls and Challenges in Clinical Metabolomics

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