Comprehensive genomic characterization of five canine lymphoid tumor cell lines

Roode, Sarah C.; Rotroff, Daniel M.; Richards, Kristy L.; Moore, Peter F.; Motsinger‐Reif, Alison A.; Okamura, Yosuke; Mizuno, Takuya; Tsujimoto, Hajime; Suter, Steven E.; Breen, Matthew

doi:10.1186/s12917-016-0836-z

Cited by 6 publications

(3 citation statements)

References 63 publications

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“…Commonly used CL cell lines include CLBL-1, CLBL-1M, GL-1, CL-1 and OSW 8 – 10 . In particular, CLBL-1 represents a canine B-cell lymphoma cell line comprehensively characterized and compared to primary neoplastic samples 11 – 14 . Nevertheless, despite the indisputable value of cell lines, clonal selection and long-term passages in vitro are known to lead to the loss of many attributes of the initial tumor, e.g.…”

Section: Introductionmentioning

confidence: 99%

Comparative High-Resolution Transcriptome Sequencing of Lymphoma Cell Lines and de novo Lymphomas Reveals Cell-Line-Specific Pathway Dysregulation

Taher

Beck²,

et al. 2018

Sci Rep

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In dogs as well as humans, lymphoma is one of the most common hematopoietic malignancies. Furthermore, due to its characteristics, canine lymphoma is recognized as a clinically relevant in vivo model to study the corresponding human disease. Immortalized cell lines are widely used as in vitro models to evaluate novel therapeutic agents and characterize their molecular mechanisms. However, it is known that long-term cultivation leads to clonal selection, genetic instability, and loss of the initial heterogenic character, limiting the usefulness of cell lines as preclinical models. Herein, we present a systematic characterization and comparison of the transcriptomic landscape of canine primary B- and T-cell lymphomas, five lymphoid cell lines (CLBL-1, CLBL-1M, GL-1, CL-1, and OSW) and four non-neoplastic control samples. We found that lymphomas and cell lines exhibit a common “differentiation and proliferation signature”. However, our analysis also showed that, independently of the cell of origin, the transcriptional signatures of lymphomas are more similar to each other than they are to those of cell lines. In particular, we observed that not all common therapeutic targets are similarly expressed between lymphomas and lymphoid cell lines, and provide evidence that different lymphoid cell-lines should be used to model distinct aspects of lymphoma dysregulation.

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Section: Introductionmentioning

confidence: 99%

Comparative High-Resolution Transcriptome Sequencing of Lymphoma Cell Lines and de novo Lymphomas Reveals Cell-Line-Specific Pathway Dysregulation

Taher

Beck²,

et al. 2018

Sci Rep

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“…Therefore, the majority of canine lymphoma xenograft murine models described until now represent T-cell lymphoid malignancies [20–22]. Notably, only four of the available cell lines are reportedly of B-cell origin, including the GL-1 cell line derived from a dog with B-cell acute lymphoblastic leukemia [34]; the 17–71, a B-cell cell line not initially phenotyped and that does not express typical B-cell lymphoma markers [35]; 3132, a cell line that probably is not of B-cell origin despite initial reports of surface immunoglobulin [36] and CLBL-1, the only available cell line that has been well-characterized both in vitro and in vivo [11,23,24,27,37]. As a matter of fact, CLBL-1 appears to be the only exclusive cell line that faithfully represents DLBCL, reproducibly inducing tumors and preserving its phenotype in the xenotransplantation setting [23,24].…”

Section: Discussionmentioning

confidence: 99%

Establishment of a bioluminescent canine B-cell lymphoma xenograft model for monitoring tumor progression and treatment response in preclinical studies

et al. 2018

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Canine diffuse large B-cell lymphoma (DLBCL) is one of the most common cancers in dogs which shares remarkable similarities with its human counterpart, making the dog an excellent model for the investigation of novel therapeutic agents. However, the integration of canine lymphoma in comparative studies has been limited due in part to the lack of suitable xenograft mouse models for preclinical studies. To overcome these limitations, we established and characterized a localized subcutaneous bioluminescent canine DLBCL xenograft mouse model. The canine CLBL-1 cell line stably expressing the luciferase and green fluorescent protein reporters was generated and used to establish the xenograft tumor model. A pilot study was first conducted with three different cell densities (0.1×106, 0.5×106 and 1×106 cells) in SCID mice. All mice presented homogeneous tumor induction within eight days after subcutaneous injection, with a 100% engraftment efficiency and no significant differences were observed among groups. The tumors were highly aggressive and localized at the site of inoculation and reproduced histological features and immunophenotype consistent with canine DLBCL. Importantly, xenograft tumors were detected and quantified by bioluminescent imaging. To assess response to therapy, a therapeutic study with a histone deacetylase inhibitor, panobinostat, was performed. The results demonstrated that panobinostat (20 mg/kg) efficiently inhibited tumor growth and that bioluminescent imaging allowed the monitorization and quantification of tumor response to therapy. In summary, this study provides a bioluminescence canine DLBCL model that offers high engraftment efficiency, preservation of tumor features, and noninvasive monitoring of tumor progression, validating the model as a promising preclinical tool for both veterinary and human medicine.

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“…The chemosensitivity assay to determine the drugs cocktail IC50 didn't work as expected for 3132 cell line and we could not evaluate the chemo-resistant potential. In addiction a recently study published that 3132 cell line, originally classified as a B-cell lymphoma, was reclassified as a histiocytic sarcoma based on characteristic cytogenomic properties (ROODE et al, 2016). This change on 3132 cells phenotype has direct implication on our results showing that a new canine lymphoma cell line should be chosen for the future experiments.…”

Section: Exosome Releases After Chemotherapymentioning

confidence: 75%

Vesículas extracelulares pequenas como marcador preditivo em cães com linfoma multicêntrico

Garnica¹

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I know you try to do your best." "I would like to thank my co-supervisor professor Juliano Coelho da Silveira for this opportunity and to introduced myself to amazing but also crazy extracellular vesicle's world. You are inspiration for me. Thank you for chats, for your patience and also for trust in me." "I also would like to thank professor Ricardo de Francisco Strefezzi for your support in pathology, for trust in our work and help since the beginning. Thank you very much!" "I would like to thank professor Andrigo Barboza de Nardi for be our first collaborator. You were fundamental for this project. Thank you very much!" "I would like to thank professor David Argyle for collaborate with us and open the doors to me. I really enjoyed my time in Scotland. Thank you very much to trust in my work." "I would like to thank doctor Lisa Pang to be my supervisor at Roslin Institute. You are brilliant and I learned too much from you. Thank you for trust in our work, for your patience and dedication. It was a very special time". "I also would like to thank our collaborators doctors Adriana Nishiya and Aline Zoppa for the opportunity of collaboration". "I would like to thank my mother for everything, for your love and cuddle. You teach the most important things. I love you mom. Thank you for always help me." "I would like to thank my fiancé Bruno Camargo de Moraes for always be patient with and hear me (it is not easy) haha. I love you so much. Thank you for staying with me in bad and moments and in all important moments of my life. You inspire me." "I would like to thank my brothers of heart Arina Lázaro Rochetti, Jéssika Cristina Chagas Lesbon and Juan Lesbon for help me every and stay with me. I am very grateful for our friendship. I am also very grateful for our dinners on Wednesday's nights. Very delicious and gourmet." "I would like to thank my family in Scotland Amanda, JV and Alana for our good times together. It was a pleasure to stay with you. Thank you very much guys, you are awesome! I miss you all." "I would like to thank Ana Clara for teach me everything about EVs. Thank you for your friendship, for be patient.

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Comprehensive genomic characterization of five canine lymphoid tumor cell lines

Cited by 6 publications

References 63 publications

Comparative High-Resolution Transcriptome Sequencing of Lymphoma Cell Lines and de novo Lymphomas Reveals Cell-Line-Specific Pathway Dysregulation

Comparative High-Resolution Transcriptome Sequencing of Lymphoma Cell Lines and de novo Lymphomas Reveals Cell-Line-Specific Pathway Dysregulation

Establishment of a bioluminescent canine B-cell lymphoma xenograft model for monitoring tumor progression and treatment response in preclinical studies

Vesículas extracelulares pequenas como marcador preditivo em cães com linfoma multicêntrico

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