Comprehensive observational assessment: Ia. A systematic, quantitative procedure for assessing the behavioral and physiologic state of the mouse

Irwin, S. T.

doi:10.1007/bf00401402

Cited by 1,181 publications

(623 citation statements)

References 3 publications

Supporting

Mentioning

606

Contrasting

Unclassified

Order By: Relevance

“…Each rat was individually placed in a transparent cage (26 × 41 cm), and 26 neurobehavioural or physiological parameters were systematically assessed according to Irwin (1968) [19] 4 and 7 weeks after the beginning of the experiment.…”

Section: Irwin Testmentioning

confidence: 99%

A rat model of FOLFOX-induced neuropathy: effects of oral dimiracetam in comparison with duloxetine and pregabalin

Mannelli

Maresca

Micheli

et al. 2017

Cancer Chemother Pharmacol

View full text Add to dashboard Cite

Results FOLFOX reduced the pain threshold in response to mechanical noxious and thermal (cold) non-noxious stimuli beginning from day 14 up to day 42 comparably to oxaliplatin alone. A fifth FOLFOX injection enhanced the severity but not the duration of painful alterations. Spontaneous activity, behavioural, autonomic, and neurological functions were also affected, whereas the motor coordination was not altered. On day 22, duloxetine (15 mg kg −1 , per os), morphine (10 mg kg −1 , subcutaneously), or pregabalin (20 mg kg −1 , per os), acutely administered, reduced the FOLFOX-dependent hypersensitivity. Repeated treatments with dimiracetam (150 mg kg −1 , per os, twice daily, from day 22) significantly protected rats from FOLFOX-induced alterations of pain threshold as well as from autonomic and neurological impairments taking effect after 7 days treatment. Pregabalin repeatedly administered (20 mg kg −1 , per os, twice daily, from day 22) was less effective in reducing mechanical hypersensitivity. Conclusion A clinically consistent model of FOL-FOX-induced neurotoxicity has been developed in rats. Dimiracetam fully reduced hypersensitivity and neurological alterations showing a relevant profile as anti-neuropathic resource.

show abstract

Section: Irwin Testmentioning

confidence: 99%

A rat model of FOLFOX-induced neuropathy: effects of oral dimiracetam in comparison with duloxetine and pregabalin

Mannelli

Maresca

Micheli

et al. 2017

Cancer Chemother Pharmacol

View full text Add to dashboard Cite

show abstract

“…1B) were not statistically different between hexb−/− and age-matched hexb+/+ mice, demonstrating that the motivation and ability to explore was intact in all mice at 66 and 79 days. Furthermore, all mice (within the age range tested) had normal pupil dilation and visual placing in the FOB (all control mice and all but one of the mutant mice reach for the grid within 2-6 cm) [12,24]. By 97 days, most hexb−/−mice showed little object exploration which resulted in a sample size too small to analyze statistically.…”

Section: Nih-pa Author Manuscriptmentioning

confidence: 99%

“…To do this, each block included animals of each condition and age. The FOB is a standard method for assessment of general arousal, reflexes, autonomic, motor and neurological deficits, and was performed as described previously [12,24]. This battery was used as a primary screen in order to verify the absence of gross deficits that would prevent or confound the principal behavioral tests.…”

Section: Introductionmentioning

confidence: 99%

“…Controls for visual acuity and exploratory deficits are intrinsic to the design of the task. We also performed a battery of tests, including assessments of motor coordination, such as the balance beam [26] and rotarod assays [3], a functional observation battery (FOB) [12,24] and open field tasks [5]. Furthermore, we tested whether deficits could be detected in relatively young mice prior to manifestation of overt symptoms.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Early deficits in motor coordination and cognitive dysfunction in a mouse model of the neurodegenerative lysosomal storage disorder, Sandhoff disease

Gulinello

Chen

Dobrenis

2008

Behavioural Brain Research

View full text Add to dashboard Cite

Mouse models of lysosomal storage diseases, including Sandhoff disease, are frequently employed to test therapies directed at the central nervous system. We backbred such mice and conducted a behavioral test battery which included sensorimotor and cognitive assessments. This is the first report of short-term memory deficits in a murine model of Sandhoff disease. We also document early onset of motor deficits using the balance beam test. KeywordsSandhoff disease; Mouse model; Novel object recognition test; Rotarod; Motor coordination; Balance beam; Open field; Memory Lysosomal storage disorders (LSDs) are a family of inherited diseases with multi-organ involvement frequently including the nervous system. The G M2 gangliosidoses, one class of human LSDs that include Tay-Sachs and Sandhoff diseases, are marked by the lysosomal accumulation of G M2 ganglioside (GM2) in neurons throughout the nervous system. Sandhoff disease arises from defects in the gene encoding the β-subunit (hexb) of β-Nacetyl-d-hexosaminide N-acetylhexosaminohydrolase (EC 3.2.1.52) normally involved in the sequential catabolism of gangliosides and other glycoconjugates [11]. Sandhoff patients have widespread brain pathology which results in clinical symptoms including progressive mental and motor deterioration [10,11,21].Two transgenic murine models of Sandhoff disease exist [23,25] that, like the human equivalent, exhibit premature death and display widespread neuronal storage of ganglioside, in addition to gliotic and inflammatory reactions and neuronal cell death [11,16,23,25,27]. The Sandhoff models have engendered much research directed at overcoming the challenges [1,2,4,11,14,15,18,20]. The knockout model [25] presented here displays ataxia, tremor, muscle wasting, and deficits in motor reflex, coordination and balance [1,4,7,8,13,14,16,18,20,22,25,27,28]. However, systematic studies examining cognitive or affective outcomes are lacking [25]. Clearly, these latter studies would be valuable to evaluate CNS-specific improvements, and would be most meaningful if assays pertinent to human neurologic assessments were employed to facilitate transition to clinical trials. Furthermore, the Sandhoff mice rarely show overt clinical signs before 3 months old and then rapidly decline, typically becoming moribund between 4 and 5 months of age [1,[13][14][15]18,20,22,25,27,28]. Therefore, behavioral assays should ideally be able to identify cognitive deficits in subjects that have multiple behavioral outcomes. In addition, increased assay sensitivity could permit behavioral assessment at earlier ages thereby also providing better evaluation of therapeutic strategies which seek earlier intervention.We Heterozygous breeders of the B6; 129S4-Hexβ tm1R1p strain [25] (Jackson Labs, Maine) were backbred through 10 generations by mating with C57BL/6J mice. Then the backbred heterozygotes were mated together and progeny genotyped using a standardized PCR protocol (provided by Jackson Labs) on tail-tip DNA extracts. Studies were performed using homozy...

show abstract

“…Similarly, the control animals in our study were given two consecutive injections of vehicle: the first one at 4 h before the testing procedure (that imitates the injection of VGB) and the second one at the time corresponding to a conventional AED tested. This procedure of two consecutive vehicle injections is a principle of behavioral studies, investigating the effects of two coinjected drugs influencing the central nervous system (Irwin, 1968). Briefly, to minimize the variance in animal behavioral responses among the examined groups, the animals were subjected to the same experimental conditions in our study.…”

Section: Drugsmentioning

confidence: 99%

Pharmacological and Behavioral Characteristics of Interactions between Vigabatrin and Conventional Antiepileptic Drugs in Pentylenetetrazole-Induced Seizures in Mice: An Isobolographic Analysis

Łuszczki

Wojcik-Cwikla

Andres

et al. 2004

Neuropsychopharmacol

View full text Add to dashboard Cite

To characterize the anticonvulsant effects and types of interactions exerted by mixtures of vigabatrin (VGB) and conventional antiepileptic drugs (valproate (VPA), ethosuximide (ESM), phenobarbital (PB), and clonazepam (CZP)) in pentylenetetrazole (PTZ)-induced seizures in mice, the isobolographic analysis for three fixed-ratio combinations of 1 : 3, 1 : 1, and 3 : 1 was used. The adverse-effect profile of the combinations tested, at the doses corresponding to their median effective doses (ED 50 ) at the fixed-ratio of 1 : 1 against PTZ-induced seizures, was determined by the chimney (motor performance), step-through passive avoidance (long-term memory), pain threshold (pain sensitivity), and Y-maze (general explorative locomotor activity) tests in mice. Additionally, the observed isobolographic interactions were verified in terms of a pharmacokinetic interaction existence. VGB combined with PB or ESM exerted supra-additive (synergistic) interactions against the clonic phase of PTZ-induced seizures, which was associated with the increment of PB or ESM concentrations in the brains of examined animals. The remaining combinations tested (ie VGB þ VPA and VGB þ CZP) occurred additive in the PTZ test, which was associated with no significant changes in the brain concentrations of VPA and CZP. None of the examined combinations exerted motor impairment in the chimney test in mice. In the standard variant of passive avoidance task (current of 0.6 mA; 2 s of stimulus duration), the combinations of VGB þ CZP and VGB þ VPA significantly affected long-term memory in mice. Moreover, VGB in a dose-dependent manner lengthened the latency to the first pain reaction in the pain threshold test in mice. The modified variant of step-through passive avoidance task (current of 0.6 mA; stimulus duration based on the latency from the pain threshold test) revealed no significant changes in the long-term memory of animals for the combinations of VGB þ VPA and VGB þ CZP; so the observed effects in the standard variant of passive avoidance task were a result of the antinociceptive effects produced by VGB. In the Y-maze test, VGB also, in a dose-dependent manner, increased the general explorative locomotor activity of the animals tested. Similarly, the total number of arm entries in the Y-maze was significantly increased for the combinations of VGB þ CZP and VGB þ ESM, but not for VGB þ PB and VGB þ VPA. The application of VGB in combination with PB, ESM, CZP, and VPA suppressed the clonic phase of PTZ-induced seizures, having no harmful or deleterious effects on behavioral functioning of the animals tested, which might be advantageous in further clinical practice.

show abstract

Comprehensive observational assessment: Ia. A systematic, quantitative procedure for assessing the behavioral and physiologic state of the mouse

Cited by 1,181 publications

References 3 publications

A rat model of FOLFOX-induced neuropathy: effects of oral dimiracetam in comparison with duloxetine and pregabalin

A rat model of FOLFOX-induced neuropathy: effects of oral dimiracetam in comparison with duloxetine and pregabalin

Early deficits in motor coordination and cognitive dysfunction in a mouse model of the neurodegenerative lysosomal storage disorder, Sandhoff disease

Pharmacological and Behavioral Characteristics of Interactions between Vigabatrin and Conventional Antiepileptic Drugs in Pentylenetetrazole-Induced Seizures in Mice: An Isobolographic Analysis

Contact Info

Product

Resources

About