Comprehensive phytohormone metabolomic and transcriptomic analysis of tobacco (Nicotiana tabacum) infected by tomato spotted wilt virus (TSWV)

Liang, Yanping; Wang, Jinfeng; Zhang, Xin; Li, Kunming; Su, Jun; Gui, Min; Li, Yongzhong; Liu, Yating

doi:10.1016/j.virusres.2024.199334

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…ICS1 promotes salicylic acid production after a tobacco infection with the virus. Pathogenesis-related gene expression can be induced by SA ( Baebler et al., 2014 ; Liang et al., 2024 ). Similarly, in Arabidopsis thaliana , vanisulfane can trigger salicylic acid accumulation, upregulate the expression of ICS1 and PR1 , and induce resistance to PVY in transgenic Arabidopsis ( Zhang et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Expression and antiviral application of exogenous lectin (griffithsin) in sweetpotatoes

Liu,

Yu,

Guo

et al. 2024

Front. Plant Sci.

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Griffithsin (GRFT) is a highly effective, broad-spectrum, safe, and stable viral inhibitor used to suppress a variety of viruses. However, little information is available on whether GRFT can prevent plant viral diseases. In this study, we constructed a GRFT overexpression vector containing the sweetpotato storage cell signal peptide and generated exogenous GRFT overexpression lines through genetic transformation. The transgenic plants showed notable resistance to sweetpotato virus disease in the virus nursery. To verify the antiplant virus function of GRFT, transient expression in tobacco leaves showed that GRFT inhibited the sweetpotato leaf curl virus (SPLCV). The replication of SPLCV was entirely inhibited when the concentration of GRFT reached a certain level. The results of pulldown and BIFC assays showed that GRFT did not interact with the six components of SPLCV. In addition, the mutated GRFTD/A without the binding ability of carbohydrate and anticoronavirus function, in which three aspartate residues at carbohydrate binding sites were all mutated to alanine, also inhibited SPLCV. Quantitative reverse-transcription PCR analyses showed that the tobacco antiviral-related genes HIN1, ICS1, WRKY40, and PR10 were overexpressed after GRFT/GRFTD/A injection. Furthermore, HIN1, ICS1, and PR10 were more highly expressed in the leaves injected with GRFTD/A. The results suggest that sweetpotato is able to express GRFT exogenously as a bioreactor. Moreover, exogenous GRFT expression inhibits plant viruses by promoting the expression of plant antiviral genes.

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Section: Discussionmentioning

confidence: 99%

Expression and antiviral application of exogenous lectin (griffithsin) in sweetpotatoes

Liu,

Yu,

Guo

et al. 2024

Front. Plant Sci.

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Diverse roles of phytohormonal signaling in modulating plant–virus interactions

Sharma,

Prasad

2024

Journal of Experimental Botany

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Virus infection brings about changes in the transcriptome, proteome and metabolome status of the infected plant wherein substantial alterations in the abundance of phytohormones and associated components involved in their signaling pathways have been observed. In the recent years, extensive research in the field of plant virology has showcased the undisputable significance of phytohormone signaling during plant-virus interactions. Apart from acting as growth regulators, phytohormones elicit robust immune response, which restricts the viral multiplication within the plant as well as its propagation by vector. Interestingly, these pathways have been shown to not only act as isolated mechanisms but as complex intertwined regulatory cascades where, the cross-talk among different phytohormones and with other antiviral pathways takes place during plant-virus interplay. Viruses cleverly disrupt phytohormone homeostasis via their multifunctional effectors that seems to be smart approach adopted by viruses to circumvent phytohormone-mediated plant immune responses. In this review, we summarize the current understanding of role of phytohormone signaling pathways during plant-virus interaction in activating antiviral immune responses of plant and also, how viruses exploit these signaling pathways favoring their pathogenesis.

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Comprehensive phytohormone metabolomic and transcriptomic analysis of tobacco (Nicotiana tabacum) infected by tomato spotted wilt virus (TSWV)

Cited by 2 publications

References 38 publications

Expression and antiviral application of exogenous lectin (griffithsin) in sweetpotatoes

Expression and antiviral application of exogenous lectin (griffithsin) in sweetpotatoes

Diverse roles of phytohormonal signaling in modulating plant–virus interactions

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