Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components

Herwijnen, Martijn J. C. van; Zonneveld, Marijke I.; Goerdayal, Soenita S.; Hoen, Esther N.M. Nolte – 't; Garssen, Johan; Stahl, Bernd; Altelaar, A.F. Maarten; Redegeld, Frank A.; Wauben, Marca H. M.

doi:10.1074/mcp.m116.060426

Cited by 144 publications

(176 citation statements)

References 45 publications

Supporting

Mentioning

164

Contrasting

Unclassified

Order By: Relevance

“…Isolation and purification of milk EVs through different methodologies (ultracentrifugation, size‐exclusion chromatography, and exoquick) allowed to discriminate between at least two EV subsets in milk including exosomes, pelleting at 100,000 g, and larger and denser EVs sedimenting at lower ultracentrifugation speeds (Benmoussa et al., , ; Théry et al., ; van Herwijnen et al., ; Wang, ).…”

Section: Cellular Origin Of Milk Micrornasmentioning

confidence: 99%

“…Nevertheless, milk EVs contain high quantities of immunity‐related microRNAs, like immune‐cell‐specific miR‐223 (Benmoussa et al., 2017, ; van Herwijnen et al., , ; Zonneveld et al., ). These can originate from all the cells present in the mammary gland (Lasser et al., ), including epithelial and immune cells.…”

Section: Cellular Origin Of Milk Micrornasmentioning

confidence: 99%

“…The majority of milk microRNAs are encapsulated within EVs that ensure their stability against harsh conditions (Benmoussa et al., , ; van Herwijnen et al., ; Wang, ). Milk microRNAs can be specifically loaded into different kinds of EVs (Shu, Chiang, Zempleni, & Cui, ; Villarroya‐Beltri et al., ), leading to specific microRNA profiles between EV subsets (Benmoussa et al., ; Chiang, Shu, Zempleni, & Cui, ; Manca et al., ; Shu et al., ), and this loading process can be influenced by the environment, by dietary habits (Paredes et al., ; Xi et al., ) or food supplementation as observed for dietary ginseng polysaccharide supplementation on porcine milk microRNAs (Sun et al., ).…”

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

See 2 more Smart Citations

Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

MicroRNAs are small noncoding RNAs responsible for regulating 40% to 60% of gene expression at the posttranscriptional level. The discovery of circulating microRNAs in several biological fluids opened the path for their study as biomarkers and long‐range cell‐to‐cell communication mediators. Their transfer between individuals in the case of blood transfusion, for example, and their high enrichment in milk have sparked the interest for microRNA transfer through diet, especially from mothers to infants during breastfeeding. The extension of such paradigm led to the study of milk microRNAs in the case of cow or goat milk consumption in adults. Here we provide a comprehensive critical review of the key findings surrounding milk microRNAs in human, cow, and goat milk among other species. We discuss the data on their biological properties, their use as disease biomarkers, their transfer between individuals or species, and their putative or verified functions in health and disease of infants and adult consumers. This work is based on all the literature available and integrates all the results, theories, debates, and validation studies available so far on milk microRNAs and related areas of investigations. We critically discuss the limitations and outline future aspects and avenues to explore in this rapidly growing field of research that could impact public health through infant milk formulations or new therapies. We hope that this comprehensive review of the literature will provide insight for all teams investigating milk RNAs’ biological activities and help ensure the quality of future reports.

show abstract

Section: Cellular Origin Of Milk Micrornasmentioning

confidence: 99%

Section: Cellular Origin Of Milk Micrornasmentioning

confidence: 99%

Section: Milk Micrornas In Health and Diseasementioning

confidence: 99%

See 1 more Smart Citation

Milk MicroRNAs in Health and Disease

Benmoussa

Provost

2019

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

show abstract

“…Other protein screening methods available such as 2D gel electrophoresis and mass spectrometry (33), are more time consuming, are poorly suited for high-throughput screening of many samples, and their sensitivity and reproducibility may prove limiting. However, milk EVs previously subjected to MS/MS for unbiased proteome profiling (34,35) have revealed several markers in common with our study, such as MIF, EpCAM, ICAM, and ITGB2. We also observed distinct protein profiles in cell lines and their corresponding exosomes, in line with previous findings using an antibody microarray called DotScan (36).…”

Section: Discussionmentioning

confidence: 97%

Tracing Cellular Origin of Human Exosomes Using Multiplex Proximity Extension Assays

Larssen

Wik

Czarnewski

et al. 2017

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

“…Re-lactation is possible but rarely discussed. Recent work shows that MOM contains >1000 proteins,35 >100 different human milk oligosaccharides,36 live cells and probiotic-type bacteria 37…”

Section: Necrotising Enterocolitis Heterogeneitymentioning

confidence: 99%

How to feed a baby recovering from necrotising enterocolitis when maternal milk is not available

Embleton

Zalewski

2017

Arch Dis Child Fetal Neonatal Ed

View full text Add to dashboard Cite

Necrotising enterocolitis (NEC) is a devastating disease with significant mortality and serious adverse outcomes in at least 50% including short gut and poor neurodevelopment. Research and management are complicated by a lack of robust clinical markers, and without histological confirmation, there is a risk of both underdiagnosis and overdiagnosis. Interunit variations in the thresholds for surgical referral, laparotomy and postmortem rates mean the actual incidence is difficult to determine, especially because the histological term 'NEC' is used in practice to describe a heterogeneous clinical syndrome. In this article, we discuss issues relating to choice of milk feed type following a clinical diagnosis of 'NEC' where mother's own milk is not available. We review common clinical concerns relating to feeding following NEC and the rationale for modifications of the macronutrient composition and quality of formula milk.

show abstract

Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components

Cited by 144 publications

References 45 publications

Milk MicroRNAs in Health and Disease

Milk MicroRNAs in Health and Disease

Tracing Cellular Origin of Human Exosomes Using Multiplex Proximity Extension Assays

How to feed a baby recovering from necrotising enterocolitis when maternal milk is not available

Contact Info

Product

Resources

About