Comprehensive qualitative and quantitative determination of pesticides and veterinary drugs in honey using liquid chromatography–Orbitrap high resolution mass spectrometry

Gómez-Pérez, María Luz; Plaza‐Bolaños, Patricia; Romero‐González, Roberto; Martı́nez-Vidal, José Luis; Garrido-Frenich, Antonia

doi:10.1016/j.chroma.2012.05.088

Cited by 159 publications

(89 citation statements)

References 25 publications

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“…Now spectrometers equipped with Orbitrap analyzers are available in diverse configurations-as a single-stage mass spectrometer and as a hybrid instrument [with a quadrupole mass filter or a linear ion trap (LTQ)]. The stand-alone instrument, which provides high mass resolution (>15,000 FMWH) and high mass accuracy (<2 ppm) but without mass selection, is already successfully used in routine for determining pesticide residues [158][159][160][161][162]. Single-stage Orbitrap-MS was demonstrated to be a very useful tool with a high potential for efficient multiresidue analysis in the field of food safety.…”

Section: Orbitrapmentioning

confidence: 99%

Advanced Mass Spectrometry

Picó

2015

Advanced Mass Spectrometry for Food Safety and Quality

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Section: Orbitrapmentioning

confidence: 99%

Advanced Mass Spectrometry

Picó

2015

Advanced Mass Spectrometry for Food Safety and Quality

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“…Although the sub-2 μm chromatographic columns allow fast separations with improved efficiencies [21], only two of their applications for determination of quinolones in food were mentioned in the reviews [22,23]. We can also add the newly published works on the development of multiclass ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods for antibiotics residue determination in chicken, fish, bee products, baby food, and in milk [14,[24][25][26][27][28], or UHPLC-Orbitrap MS for veterinary drugs determination in honey [29]. A multiclass determination of 41 antibiotics in one chromatographic run of 13 min [29] and determination of 23 quinolones in 10 min [14] was achieved.…”

Section: Introductionmentioning

confidence: 99%

“…We can also add the newly published works on the development of multiclass ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods for antibiotics residue determination in chicken, fish, bee products, baby food, and in milk [14,[24][25][26][27][28], or UHPLC-Orbitrap MS for veterinary drugs determination in honey [29]. A multiclass determination of 41 antibiotics in one chromatographic run of 13 min [29] and determination of 23 quinolones in 10 min [14] was achieved. Another trend in chromatographic separation described by Nunez et al [19] and Farré et al [20] is the use of monolith columns, which provide higher separation efficiency than in the case of fully porous columns.…”

Section: Introductionmentioning

confidence: 99%

Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection

Stoilova¹,

Surleva²,

Stoev³

2014

Acta Chromatographica

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Summary. High-performance liquid chromatography coupled with fluorescence (HPLC-FD) and tandem mass spectrometric detection (LC-MS/MS) was studied as a versatile tool for fast and reliable determination of nine regulated quinolones in food of animal origin (Council Regulation 2377/90/ECC). The sample pre-treatment protocol includes double step extraction with acetonitrile followed by solid phase extraction (SPE) cleanup on hydrophobic-lipophilic balance (HLB) cartridge. The separation of quinolones in HPLC-FD determination was performed on C18 Zorbax column with a gradient mixture of aqueous formic acid, methanol, and acetonitrile. A multi-wavelength excitation/emission program was used for sensitive quinolones detection. The separation efficiency of newly available chromatographic columns: Gemini C18 and Synergi Polar RP (fully porous particles), as well as Kinetex PFP and Poroshell 120 EC-C18 (coreshell particles), was studied in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis. Appropriate gradient elution program was designed for each column. Multiple reaction monitoring was used for selective determination of each quinolone. LC-MS/MS allowed quinolones determination in less than 5 min. Both methods showed detection limits below maximum residue limits for quinolones residues in food commodities.

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“…Pesticide use has greatly increased in recent decades for their effects on crop protection from the harmful activity of parasites (Gómez-Pérez et al 2012). In fact, many of these pulverized pesticides are scattered in the environment and can then contaminate water, area, soil and food (Tette et al 2016).…”

Section: Introductionmentioning

confidence: 99%

The use of honey as environmental biomonitor of pesticides contamination in northern Lebanon

Al-Alam

Fajloun

Chabni

et al. 2017

Euro-Mediterr J Environ Integr

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The intensive use of pesticides in agriculture generates a contamination of the environment that must be assessed. Bee products such as honey, widely consumed, are not spared by this considerable contamination that can lead to health risks. Bees and their products (honey, nectar, pollen, etc) are considered as real indicators of environmental health. Indeed, these insects, characterized by a specified body and a high mobility, are considered particularly suitable to keep, with high sensitivity, pesticides with which they come in contact. So the analysis of these insects or of their products as honey has been demonstrated as a good bio indicator of environmental quality. In this study, 18 samples of honey collected from North Lebanon, were assayed for their contamination by 84 pesticides. Multi residue extraction was based on QuEChERS, followed by chromatographic analysis by LC-MS/MS and GC-MS/MS. For gas chromatographic analysis, samples were concentrated by SPME before being injected to the GC column. At first, the method was developed and validated using organic honey, and then the collected samples were analyzed. The limits of detection and quantification as well as the repeatability and the reproducibility of the method have been defined: between 0.1 and 25.2 ng g -1 and between 0.6 to 83.1 ng g -1 for the limits detection and quantification respectively. Furthermore, the repeatability was between 0.2 and 15% and the reproducibility between 0.2 and 18%. Concerning the analyzed samples, residues of many pesticides have been found in each sample. Samples collected from the plain of Akkar and the Byblos area, widely known for their agricultural productions, were the most contaminated by pesticides. In fact, pesticide levels found in honey collected from Akkar and Byblos were 1753.92 and 695.13 ng g -1 respectively. Contrariwise, samples collected from the Cedars of Lebanon had the lowest pesticides concentration with a total concentration of 19.5 ng g -1 .In conclusion, this study shows that honey can indicate the specific pollution of a given environment and can be considered as an appropriated sentinel for environmental biomonitoring.

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Comprehensive qualitative and quantitative determination of pesticides and veterinary drugs in honey using liquid chromatography–Orbitrap high resolution mass spectrometry

Cited by 159 publications

References 25 publications

Advanced Mass Spectrometry

Advanced Mass Spectrometry

Determination of quinolonones in food of animal origin by liquid chromatography coupled with fluorescence and mass spectrometric detection

The use of honey as environmental biomonitor of pesticides contamination in northern Lebanon

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