Comprehensive review on agro technologies of low‐calorie natural sweetener stevia (<i>Stevia rebaudiana</i>Bertoni): a boon to diabetic patients

Sharma, Saurabh; Walia, Shanka; Singh, Bikram; Kumar, Rakesh

doi:10.1002/jsfa.7500

“…After analysis, a total of six SSR types can be detected, including mononucleotide, dinucleotide, trinucleotide, tetranucleotide, pentanucleotide, and hexanucleotide SSRs. In addition, the repeat times were divided into 5,6,7,8,9,10,11, and > 11 times.…”

Section: Simple Sequence Repeat (Ssr) Analysismentioning

confidence: 99%

Characterizing glycosyltransferases by a combination of sequencing platforms applied to the leaf tissues of Stevia rebaudian

Zhang

¹

,

Liu

²

,

Lyu

³

et al. 2020

Preprint

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Background: Stevia rebaudiana (Bertoni) is considered one of the most valuable plants because of the steviol glycosides (SGs) that can be extracted from its leaves. Glycosyltransferases (GTs), which can transfer sugar moieties from activated sugar donors onto saccharide and nonsaccharide acceptors, are widely distributed in the genome of S. rebaudiana and play important roles in the synthesis of steviol glycosides.Results: Six stevia genotypes with significantly different concentrations of SGs were obtained by induction through various mutagenic methods, and the contents of seven glycosides (stevioboside, Reb B, ST, Reb A, Reb F, Reb D and Reb M) in their leaves were considerably different. Then, NGS and single-molecule real-time (SMRT) sequencing were combined to analyse leaf tissue from these six different genotypes to generate a more complete and correct full-length transcriptome of S. rebaudiana. Two phylogenetic trees of glycosyltransferases (SrUGTs) were constructed by the neighbour-joining method and successfully predicted the functions of SrUGTs involved in SG biosynthesis. With further insight into glycosyltransferases (SrUGTs) involved in SG biosynthesis, the weighted gene co-expression network analysis (WGCNA) method was used to characterize the relationships between SrUGTs and SGs, and forty-four potential SrUGTs were finally obtained. Of these potential SrUGTs, twenty-seven have complete ORFs and have not been verified to date, and enzyme assays were subsequently performed, but none of them had activity towards SGs. In addition, SrUGT (SrUGT88B1-1) could utilize UDP-glucose as a sugar donor to glycosylate isoquercetin to form three products containing one, two, and three glucoses, respectively. Conclusion:Combined with the results obtained by previous studies and those of this work, we systematically characterized glycosyltransferases in S. rebaudiana and confirmed that four enzymes (SrUGT85C2, SrUGT74G1, SrUGT76G1 and SrUGT91D2) are primarily involved in the glycosylation of steviol glucosides. Moreover, the complete and correct full-length transcriptome obtained in this study will provide valuable support for further research investigating S. rebaudiana.(S. rebaudiana and S. phlebophylla) in this genus to produce steviol glycosides (SGs), compounds that appeal to people looking for more natural plant-based ingredients in their diet because of their natural-origin, plant-based and zero-calorie sustainable sweeteners [1,2]. Indeed, finding nonnutritive sweeteners to reduce sugar intake is one valuable path to solve some serious health problems at present, especially for those with obesity or diabetes. Therefore, making stevia a leaf crop with significant economic value and the food industry has a very positive outlook regarding the opportunities for SGs. Consequently, the value of the SG market is expected to exceed $1 billion USD by 2021 [3]. To date, more than thirty-five SGs have been isolated and identified from S. rebaudiana, including steviolbioside, rebaudioside A-Q (Reb A-Q), 1,2-stevioside (...

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“…After analysis, a total of six SSR types can be detected, including mononucleotide, dinucleotide, trinucleotide, tetranucleotide, pentanucleotide, and hexanucleotide SSRs. In addition, the repeat times were divided into 5,6,7,8,9,10,11, and > 11 times.…”

Section: Simple Sequence Repeat (Ssr) Analysismentioning

confidence: 99%

Characterizing glycosyltransferases by a combination of sequencing platforms applied to the leaf tissues of Stevia rebaudiana

Zhang

¹

,

Liu

²

,

Lyu

³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Background: Stevia rebaudiana (Bertoni) is considered one of the most valuable plants because of the steviol glycosides (SGs) that can be extracted from its leaves. Glycosyltransferases (GTs), which can transfer sugar moieties from activated sugar donors onto saccharide and nonsaccharide acceptors, are widely distributed in the genome of S. rebaudiana and play important roles in the synthesis of steviol glycosides.Results: Six stevia genotypes with significantly different concentrations of SGs were obtained by induction through various mutagenic methods, and the contents of seven glycosides (stevioboside, Reb B, ST, Reb A, Reb F, Reb D and Reb M) in their leaves were considerably different. Then, NGS and single-molecule real-time (SMRT) sequencing were combined to analyse leaf tissue from these six different genotypes to generate a more complete and correct full-length transcriptome of S. rebaudiana . Two phylogenetic trees of glycosyltransferases (SrUGTs) were constructed by the neighbour-joining method and successfully predicted the functions of SrUGTs involved in SG biosynthesis. With further insight into glycosyltransferases (SrUGTs) involved in SG biosynthesis, the weighted gene co-expression network analysis (WGCNA) method was used to characterize the relationships between SrUGTs and SGs, and forty-four potential SrUGTs were finally obtained, including SrUGT85C2 , SrUGT74G1 , SrUGT76G1 and one SrUGT91D2, which have already been reported to be involved in the glucosylation of steviol glycosides, illustrating the reliability of our results.Conclusion: Combined with the results obtained by previous studies and those of this work, we systematically characterized glycosyltransferases in S. rebaudiana and forty-four candidate SrUGTs involved in the glycosylation of steviol glucosides were obtained. Moreover, the complete and correct full-length transcriptome obtained in this study will provide valuable support for further research investigating S. rebaudiana .

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“…Uno de los más utilizados recientemente, es la Stevia rebaudiana bertoni, planta nativa de Paraguay. Se le ha considerado como un bio-edulcorante utilizado durante siglos con propiedades medicinales, de potencial efecto hipoglicemiante (14,15). A pesar de que la estevia puede ser útil para cualquier persona, ciertos grupos tienen más probabilidades de beneficiarse de sus propiedades tales como pacientes diabéticos, sujetos interesados en disminuir la ingesta calórica y niños.…”

Section: Introductionunclassified

“…A pesar de que los valores de IG y CG de diversos productos alimenticios a nivel mundial se han publicado en una tabla internacional elaborada por Atkinson (21), aún existen gran cantidad de estos suplementos nutricionales líquidos en los que estos valores no se han determinado (15). El estudio de los indicadores de la respuesta glicémica a los alimentos generalmente se realiza en sujetos sanos, con el fin de determinar un comportamiento metabólico que sirva de referencia para comparar con diabéticos (10).…”

Section: Introductionunclassified

Rol De La Stevia Y L-Carnitina Sobre El Impacto Glicémico De Un Suplemento Nutricional en Adultos

Angarita

¹

,

Agüero

²

,

Apaicio

³

et al. 2017

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Rol de la estevia y L-carnitina sobre el impacto glicémico de un suplemento nutricional en adultos Role of the stevia and L-carnitine of a nutritional supplement on glycemic impact in adults ResumenActualmente la industria alimentaria ha generado interés en edulcorantes no nutritivos, por ejemplo la estevia y en componentes especiales como la L-carnitina, utilizados en formulaciones de suplementos nutricionales para el control glicémico específicos para diabéticos. El presente estudio evaluó el efecto de la estevia y la L-carnitina sobre el índice glicémico (IG) y la carga glicémica (CG) de un suplemento nutricional en 19 sujetos sanos (9 hombres y 10 mujeres), quienes completaron aleatoriamente 3 pruebas de consumo, 1 para el suplemento y 1 para cada producto de referencia: solución glucosada (SG) y pan blanco (PB), obteniendo muestras de sangre a los tiempos 0, 15, 30, 45, 60, 90 y 120 min; para medición de glicemias, e insulina basal y postprandial. El área de incremento bajo la curva de glucosa (IAUC) fue menor para el suplemento 11.778,73 que para los productos de referencia (SG) 13.724,06; (PB) 13.153,56 α = p 0,005. El IG = (62) y la CG = (16) resultaron intermedios y más bajos que el del pan blanco IG = (69) y la CG = (18), sin diferencias en la insulina postprandial. Esto demuestra que este suplemento nutricional formulado con estevia y L-carnitina es capaz de prolongar la respuesta glicémica sin aumentar los requerimientos insulínicos en sujetos sanos. Se requieren estudios específicos en diabéticos para validar si el impacto glicémico es menor que el producto patrón. La presencia de otros nutrientes en la fórmula, influyentes en estos indicadores, no permite inferir que los resultados se deban únicamente al tipo de endulzante utilizado y a la L-carnitina. Key words:Supplement. Stevia. L-carnitine. Glycemia. AbstractCurrently the food industry has generated interest in non-nutritive sweeteners, for example Stevia and in special components such as L-carnitine, used in formulations of nutritional supplements for glycemic control specific for diabetics. The present study evaluated the effect of stevia and L-carnitine on the glycemic index (GI) and glycemic load (CG) of a nutritional supplement in 19 healthy subjects (9 men and 10 women), who randomly completed 3 consumption tests, 1 for the supplement and 1 for each reference product: Glucose solution (SG) and white bread (PB), obtaining blood samples at the 0, 15, 30, 45, 60, 90 and 120 min times; for measurement of blood glucose, basal and postprandial insulin. The increase area under the glucose curve (IAUC) was lower for supplement 11,778.73 than for reference products (SG) 13,724.06; (PB) 13,153.56 α = p 0.005. IG = (62) and CG = (16) were intermediate and lower than white bread IG = (69) and CG = (18), with no difference in postprandial insulin. This demonstrates that this nutritional supplement formulated with stevia and L-carnitine is able to prolong the glycemic response without increasing the insulin requirements in healthy subjects. Specific s...

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Comprehensive review on agro technologies of low‐calorie natural sweetener stevia (Stevia rebaudianaBertoni): a boon to diabetic patients

Cited by 32 publications

References 76 publications

Characterizing glycosyltransferases by a combination of sequencing platforms applied to the leaf tissues of Stevia rebaudian

Characterizing glycosyltransferases by a combination of sequencing platforms applied to the leaf tissues of Stevia rebaudian

Characterizing glycosyltransferases by a combination of sequencing platforms applied to the leaf tissues of Stevia rebaudiana

Rol De La Stevia Y L-Carnitina Sobre El Impacto Glicémico De Un Suplemento Nutricional en Adultos

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