Comprehensive screening and selection of okra (Abelmoschus esculentus) germplasm for salinity tolerance at the seedling stage and during plant ontogeny

Haq, Ikramul; Khan, Asif Ali; Khan, Iqrar Ahmad; Azmat, Muhammad

doi:10.1631/jzus.b1200027

Cited by 14 publications

(8 citation statements)

References 41 publications

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“…The assessment of salinity tolerant and sensitive genotypes on the basis of germination, seedling growth and biomass accumulation, salinity scores, seedling survivability, Na + , Cl - and K + at seedling stage under hydroponics and Na + , Cl - , K + concentrations, pods/plant and seed yield parameters at maturity under saline field, showed consistency in the salinity tolerance in selected genotypes until maturity. This phenomenon has been observed in number of crops [ 76 ]. Similar strategy has been followed in faba bean for the selection of genotypes for salinity tolerance under both hydroponic and field conditions [ 77 ].…”

Section: Discussionmentioning

confidence: 76%

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Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Singh

Kumari

et al. 2017

PLoS ONE

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One hundred and sixty two genotypes of different Lens species were screened for salinity tolerance in hydroponics at 40, 80 and 120 mM sodium chloride (NaCl) for 30 d. The germination, seedling growth, biomass accumulation, seedling survivability, salinity scores, root and shoot anatomy, sodium ion (Na+), chloride ion (Cl-) and potassium ion (K+) concentrations, proline and antioxidant activities were measured to evaluate the performance of all the genotypes. The results were compared in respect of physiological (Na+, K+ and Cl-) and seed yield components obtained from field trials for salinity stress conducted during two years. Expression of salt tolerance in hydroponics was found to be reliable indicator for similarity in salt tolerance between genotypes and was evident in saline soil based comparisons. Impressive genotypic variation for salinity tolerance was observed among the genotypes screened under hydroponic and saline field conditions. Plant concentrations of Na+ and Cl- at 120 mM NaCl were found significantly correlated with germination, root and shoot length, fresh and dry weight of roots and shoots, seedling survivability, salinity scores and K+ under controlled conditions and ranked the genotypes along with their seed yield in the field. Root and shoot anatomy of tolerant line (PDL-1) and wild accession (ILWL-137) showed restricted uptake of Na+ and Cl- due to thick layer of their epidermis and endodermis as compared to sensitive cultigen (L-4076). All the genotypes were scanned using SSR markers for genetic diversity, which generated high polymorphism. On the basis of cluster analysis and population structure the contrasting genotypes were grouped into different classes. These markers may further be tested to explore their potential in marker-assisted selection.

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Section: Discussionmentioning

confidence: 76%

“…This phenomenon has been observed in number of crops [76]. Similar strategy has been followed in faba bean for the selection of genotypes for salinity tolerance under both hydroponic and field conditions [77].…”

Section: Discussionmentioning

confidence: 84%

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Singh

Kumari

et al. 2017

PLoS ONE

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“…The maximum shoot length was in T 0 (11.88 cm) and the minimum was in T 4 (1.13 cm). Similar results were reported in cucumber (Marium et al, 2019) [10] and bhendi (Abbas et al, 2014; Haq et al, 2012) [12,11] .…”

Section: Effect Of Salinity In Shoot Length (Cm)supporting

confidence: 82%

“…But in T 4 a sudden decline is observed which was 25 %. Increasing salt concentrations significantly affected the germination percentage in bhendi (Haq et al, 2012) [11] .…”

Section: Resultsmentioning

confidence: 99%

In vitro evaluation of tomato (Lycopersicon esculentum Mill.), chilli (Capsicum annum L.), cucumber (Cucumis sativus L.) and Bhendi (Abelmoschus esculentus L.) for salinity stress

Loganayaki¹,

Tamizhmathi²,

Brinda³

et al. 2020

Int. J. Chem. Stud.

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Salinity is one of the most important abiotic stresses limiting yield potential. Salinity is even more harmful to seed germination and seedling development. The present study aimed to evaluate the effect of the salinity on germination and seedling growth in four different vegetable crops viz., tomato, chilli, cucumber and bhendi. In this study, germination percentage (%), shoot length (cm), root length (cm), root to shoot ratio, fresh and dry weight (g) were recorded at four different salinity levels (50 mM, 100 mM, 150 mM and 200 mM NaCl) and one control (0 mM NaCl). Significant differences were observed in all the traits in all crops in this study. Based on the germination percentage, by comparing the four crops, cucumber had the highest tolerance to the salinity which was followed by bhendi. The crops of tomato and chilli were more sensitive to salinity.

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“…According to the study performed by Greenway and Munns, 1980 [ 45 ], the decrease in biomass was also due to decreased water potential in plants cells under drought stress condition, leading to a loss in cell turgidity, which reduced the overall cell elongation and cell division activity in plants. The differences in the genetic makeup of each okra genotype, and the climatic factors, were responsible for the difference in biomass (root and shoot length, and root and shoot weight) of all the four okra genotypes [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Target-Based Physiological Modulations and Chloroplast Proteome Reveals a Drought Resilient Rootstock in Okra (Abelmoschus esculentus) Genotypes

Razi

Bae

Muneer

2021

IJMS

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As climate changes increase, drought stress is becoming a problem for all major horticultural crops; among them is okra (Abelmoschus esculentus). Despite its superior resilience to heat stress and high nutritional content, it is still underutilized in contrast to other vegetable crops. Moreover, the drought-resistant and drought-sensitive genotypes of okra are also not well known and require further exploration to improve their productivity. To investigate this in more detail, we performed comparative physiological and large-scale chloroplast proteomics on drought-stressed genotypes of okra. We evaluated four major genotypes of okra, viz., NS7774, NS7772, Green Gold, and OH3312 for drought resilient rootstock. The physiological modulations demonstrated a significant change by 50–76% in biomass, net-photosynthetic machinery, water transport, and absorption both in early and late stages of drought stress compared to well-watered crops in all genotypes. Maximum oxidative damage due to drought stress was observed for the genotypes NS7772, Green Gold and OH3312 as depicted by H2O2 and O2− determination. Greater oxidative stress was correlated to lesser antioxidant activity and expression of antioxidant enzymes, such as catalase and ascorbate peroxidase under stress in okra genotypes. The overall photosynthetic pigments, such as total chlorophyll, and total carotenoid content, were also decreased, and stomatal guard cells were disrupted and appeared closed compared to the control for the above three mentioned genotypes, except NS7774. A subsequent tissue-specific proteome analysis of chloroplasts and thylakoids analyzed by BN-PAGE (blue native polyacrylamide gel electrophoresis) revealed either over or under expression of specific proteins, such as ATPase, PSI, PSII core dimer, PSII monomer and ATP synthase. The expression of multiprotein complex proteins, including PSII-core dimer and PSII-core monomer, was slightly higher for the genotype NS7774 when compared to three other genotypes for both 5 and 10 days of drought stress. Further identification of specific proteins obtained in second dimension BN-PAGE provided descriptive detail of seven proteins involved in drought resistance across all genotypes. The identified proteins are majorly involved in photosynthesis under drought stress, suggesting NS7774 as a drought tolerant genotype. Further, the proteomic results were confirmed using Immunoblot by selecting specific protein such as PsaA. Overall, from our physiological modulations and chloroplast proteomics in all genotypes, we summarized NS7774 as a resilient rootstock and the other three genotypes (NS7772, OH3312, and Green Gold) as sensitive ones.

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Comprehensive screening and selection of okra (Abelmoschus esculentus) germplasm for salinity tolerance at the seedling stage and during plant ontogeny

Cited by 14 publications

References 41 publications

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

In vitro evaluation of tomato (Lycopersicon esculentum Mill.), chilli (Capsicum annum L.), cucumber (Cucumis sativus L.) and Bhendi (Abelmoschus esculentus L.) for salinity stress

Target-Based Physiological Modulations and Chloroplast Proteome Reveals a Drought Resilient Rootstock in Okra (Abelmoschus esculentus) Genotypes

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