Comprehensive step-by-step procedure to setup a molecular communication through liquid experiment

Calì, Federico; Fichera, Luca; Tuccitto, Nunzio

doi:10.1016/j.mex.2022.101736

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…In parallel, the choice of CNDs, a relatively new type of carbon-based nanoparticles (1-10 nm in diameter), is supported by the fact that they offer diverse physicochemical properties and advantageous characteristics such as biocompatibility, low cytotoxicity, electrical conductivity, ease of synthesis, abundant functional groups (e.g., amino, hydroxyl, carboxyl), and high physicochemical stability. [42][43][44][45][46][47][48] CNDs can be synthesized from a wide variety of carbon-containing precursors all of which dictate the resultant surface functional groups. [49] Because they are a relatively new class of nanoparticles, the potential of CNDs in the context of neural tissue engineering is only beginning to be explored.…”

Section: Introductionmentioning

confidence: 99%

Electroconductive Collagen‐Carbon Nanodots Nanocomposite Elicits Neurite Outgrowth, Supports Neurogenic Differentiation and Accelerates Electrophysiological Maturation of Neural Progenitor Spheroids

Lomboni,

Ozgun,

de Medeiros

et al. 2023

Adv Healthcare Materials

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Neuronal disorders are characterized by the loss of functional neurons and disrupted neuroanatomical connectivity, severely impacting the quality of life of patients. This study investigates a novel electroconductive nanocomposite consisting of glycine‐derived carbon nanodots (GlyCNDs) incorporated into a collagen matrix and validates its beneficial physicochemical and electro‐active cueing to relevant cells. To this end, this work employs mouse induced pluripotent stem cell (iPSC)‐derived neural progenitor (NP) spheroids. The findings reveal that the nanocomposite markedly augmented neuronal differentiation in NP spheroids and stimulate neuritogenesis. In addition, this work demonstrates that the biomaterial‐driven enhancements of the cellular response ultimately contribute to the development of highly integrated and functional neural networks. Lastly, acute dizocilpine (MK‐801) treatment provides new evidence for a direct interaction between collagen‐bound GlyCNDs and postsynaptic N‐methyl‐D‐aspartate (NMDA) receptors, thereby suggesting a potential mechanism underlying the observed cellular events. In summary, the findings establish a foundation for the development of a new nanocomposite resulting from the integration of carbon nanomaterials within a clinically approved hydrogel, toward an effective biomaterial‐based strategy for addressing neuronal disorders by restoring damaged/lost neurons and supporting the reestablishment of neuroanatomical connectivity.

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