Computational Identification and Characterization of New microRNAs in Human Platelets Stored in a Blood Bank

Maués, Jersey Heitor da Silva; Moreira-Nunes, Caroline Aquino; Burbano, Rommel Rodrı́guez

doi:10.3390/biom10081173

Cited by 4 publications

(4 citation statements)

References 70 publications

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“…As platelets suffer storage damage or are activated by the presence of microorganisms, there is an initial process of the degradation of miRNAs that reduces their quantity. However, this process ceases, and some miRNAs may have their quantity increased as the cell damage or sepsis progresses, as RNA-editing enzymes such as RNase and RNA helicases increase the quantity of certain miRNAs by cleaving miRNA precursors (pre-miRNAs) into mature miRNAs in response to oxidative stress [ 8 , 10 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…When platelets are not activated (that is, when they are in a physiologically normal stage), the quantity of miR-127 is greater than that of miR-320a. When the platelet concentrate (PC) stored in a blood bank undergoes aging due to excessive storage time or contamination by microbial agents, the degradation of miR-320a is lower than that of miR-127, and as a result miR-320a is present at a higher concentration [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens

Corrêa

Carneiro²,

Valente

et al. 2021

Genes

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The present study proposes to legitimize in sepsis a characteristic found in platelets that suffer storage lesions in blood banks, which is the increased expression of miRNA miR-320a in relation to miR-127. Under physiologically normal conditions, an inverse relationship is observed. The aim of this study was to verify whether the analysis of miR-320a and miR-127 expression in platelets could detect a decrease in their viability and function due to the presence of pathogens in the blood of patients hospitalized in the Intensive Care Unit. We also investigated the expression of membrane antigens sensitive to platelet activation. Of the 200 patients analyzed, only those who developed sepsis (140) were found to have a higher relative quantity of miR-320a than that of miR-127. This characteristic and the increased expression of membrane antigens P2Y12, CD62P, CD41, and CD61 showed a significant association (p < 0.01) with all types of sepsis evaluated in this study. Additionally, 40% of patients hospitalized for sepsis had negative results for the first cultures. We conclude that analysis of miR-127 and miR-320a expression combined with membrane antigens evaluation, in association with the available clinical and diagnostic parameters, are important tools to detect the onset of sepsis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens

Corrêa

Carneiro²,

Valente

et al. 2021

Genes

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“…One limitation is the updating of precursor and mature microRNA sequence databases. A recent study, 60 based on the latest version of miRbase, comprehensively summarizes the diverse repertoire of miRNAs that are identified in platelets stored in blood banks for 6 days. These findings are further validated using quantitative real‐time polymerase chain reaction (qPCR).…”

Section: Psls At 20–24°c Under Constant Horizontal Agitationmentioning

confidence: 99%

The platelet storage lesion, what are we working for?

Liu,

Su,

Guo

et al. 2023

Clinical Laboratory Analysis

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BackgroundPlatelet concentrate (PC) transfusions are crucial in prevention and treatment of bleeding in infection, surgery, leukemia, and thrombocytopenia patients. Although the technology for platelet preparation and storage has evolved over the decades, there are still challenges in the demand for platelets in blood banks because the platelet shelf life is limited to 5 days due to bacterial contamination and platelet storage lesions (PSLs) at 20–24°C under constant horizontal agitation. In addition, the relations between some adverse effects of platelet transfusions and PSLs have also been considered. Therefore, understanding the mechanisms of PSLs is conducive to obtaining high quality platelets and facilitating safe and effective platelet transfusions.ObjectiveThis review summarizes developments in mechanistic research of PSLs and their relationship with clinical practice, providing insights for future research.MethodsAuthors conducted a search on PubMed and Web of Science using the professional terms “PSL” and “platelet transfusion.” The obtained literature was then roughly categorized based on their research content. Similar studies were grouped into the same sections, and further searches were conducted based on the keywords of each section.ResultsDifferent studies have explored PSLs from various perspectives, including changes in platelet morphology, surface molecules, biological response modifiers (BMRs), metabolism, and proteins and RNA, in an attempt to monitor PSLs and identify intervention targets that could alleviate PSLs. Moreover, novel platelet storage conditions, including platelet additive solutions (PAS) and reconsidered cold storage methods, are explored. There are two approaches to obtaining high‐quality platelets. One approach simulates the in vivo environment to maintain platelet activity, while the other keeps platelets at a low activity level in vitro under low temperatures.ConclusionUnderstanding PSLs helps us identify good intervention targets and assess the therapeutic effects of different PSLs stages for different patients.

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“…Therefore, it is essential to screen molecular biomarkers to assess and monitor the physiological viability of platelets in PC. Maués et al [ 4 ] used an approach to identify miRNAs in normal human platelet sRNA-Seq data from the GSE61856 repository. A new collection of miRNAs (miR-486-5p, miR-92a-3p, miR-103a-3p, miR-151a-3p, miR-181a-5p, and miR-221-3p) was identified by their study that showed a sensitivity expression pattern due to biological platelet changes during storage.…”

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confidence: 99%