Computational sensing of herpes simplex virus using a cost-effective on-chip microscope

Abstract

Caused by the herpes simplex virus (HSV), herpes is a viral infection that is one of the most widespread diseases worldwide. Here we present a computational sensing technique for specific detection of HSV using both viral immuno-specificity and the physical size range of the viruses. This label-free approach involves a compact and cost-effective holographic on-chip microscope and a surface-functionalized glass substrate prepared to specifically capture the target viruses. To enhance the optical signatures of individual viruses and increase their signal-to-noise ratio, self-assembled polyethylene glycol based nanolenses are rapidly formed around each virus particle captured on the substrate using a portable interface. Holographic shadows of specifically captured viruses that are surrounded by these self-assembled nanolenses are then reconstructed, and the phase image is used for automated quantification of the size of each particle within our large field-of-view, ~30 mm2. The combination of viral immuno-specificity due to surface functionalization and the physical size measurements enabled by holographic imaging is used to sensitively detect and enumerate HSV particles using our compact and cost-effective platform. This computational sensing technique can find numerous uses in global health related applications in resource-limited environments.

Detection of Bacterial and Viral Pathogens Using Photonic Point-of-Care Devices

“…In another example, a chip for the capture of HSV-1 virus was prepared by first functionalizing the glass with silane-PEG-biotin and then adding streptavidin to it, as shown in Figure 7 [ 152 ]. Non-specific binding was eliminated by coating the glass coverslip with m-PEG-silane.…”

“…( B ) Schematic of the portable lensless microscope with pixel super-resolution capability. The device weighs less than 500 gm and is about 25 cm in height [ 152 ]. Lensless microscopy was also used to detect Staphylococcus aureus directly in a contact lens [ 154 ].…”

“…Deep learning approaches were used for image reconstruction and analysis, which yielded a limit of detection as low as ~5 viral copies/µL (i.e., ~25 copies/test). In another example, a chip for the capture of HSV-1 virus was prepared by first functionalizing the glass with silane-PEG-biotin and then adding streptavidin to it, as shown in Figure 7 [152]. Nonspecific binding was eliminated by coating the glass coverslip with m-PEG-silane.…”

“…A limit of detection of 120 viral particles per test over a field of view of ~30 mm 2 was reported. Another approach of detecting pathogens was demonstrated by In another example, a chip for the capture of HSV-1 virus was prepared by first functionalizing the glass with silane-PEG-biotin and then adding streptavidin to it, as shown in Figure 7 [152]. Non-specific binding was eliminated by coating the glass coverslip with m-PEG-silane.…”

Desenvolvimento e caracterização de um microscópio óptico holográfico sem lentes <i>in-line</i>

“…Devido às suas características desenvolvidas para atuação em campo, esses instrumentos também encontraram aplicações em avaliação de partículas do ar, 43 análise de fluidos 44 e diagnósticos clínicos. 18, [45][46][47][48] Tendo também montagens desenvolvidas para o uso conjunto com aparelhos celulares. [43][44] As várias aplicações desses sistemas sugerem modificações na montagem dos E, então, a resolução é dada por 5 :…”