Computational study of biochemical properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) enzyme in C3 plants

Darabi, Maryam; Seddigh, Samin

doi:10.1007/s12374-016-0211-4

Cited by 8 publications

(4 citation statements)

References 43 publications

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“…Proteomics analysis suggested that many proteins were increased or decreased in the McHB7 overexpressing plants compared to WT. For example, K9NCW5 (Rubisco), a key enzyme involved in the first step of carbon fixation in photosynthesis [47]. It was reported that the small subunit of Rubisco was coded by nuclear genes [48,49].…”

Section: Discussionmentioning

confidence: 99%

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Zhang

Tan

Zhu

et al. 2021

IJMS

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Mesembryanthemum crystallinum (common ice plant) is a halophyte species that has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The McHB7 protein was targeted to the nucleus. McHB7-overexpressing in ice plant leaves through Agrobacterium-mediated transformation led to 25 times more McHB7 transcripts than the non-transformed wild type (WT). After 500 mM NaCl treatment for 7 days, the activities of superoxide dismutase (SOD) and peroxidase (POD) and water content of the transgenic plants were higher than the WT, while malondialdehyde (MDA) was decreased in the transgenic plants. A total of 1082 and 1072 proteins were profiled by proteomics under control and salt treatment, respectively, with 22 and 11 proteins uniquely identified under control and salt stress, respectively. Among the 11 proteins, 7 were increased and 4 were decreased after salt treatment. Most of the proteins whose expression increased in the McHB7 overexpression (OE) ice plants under high salinity were involved in transport regulation, catalytic activities, biosynthesis of secondary metabolites, and response to stimulus. The results demonstrate that the McHB7 transcription factor plays a positive role in improving plant salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Zhang

Tan

Zhu

et al. 2021

IJMS

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“…The protein sequences of OsGUX1 ZS and OsGUX1 AC were aligned with the homologous proteins that were retrieved from Phytozome v12.1 database 54 . The multiple sequence alignment of the full-length proteins was performed in the complete alignment method by the ClustalX in MEGA 6 55 . The unrooted phylogeny tree was developed using the neighbor-joining method in the software MEGA 6. phenotypic measurements.…”

Section: Subcellular Localization Of Osgux1 Protein For Investigatinmentioning

confidence: 99%

A xylan glucuronosyltransferase gene exhibits pleiotropic effects on cellular composition and leaf development in rice

Gao

Sun

Wang

et al. 2020

Sci Rep

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Leaf chlorophyll content is an important physiological indicator of plant growth, metabolism and nutritional status, and it is highly correlated with leaf nitrogen content and photosynthesis. in this study, we report the cloning and identification of a xylan glucuronosyltransferase gene (OsGUX1) that affects relative chlorophyll content in rice leaf. Using a set of chromosomal segment substitution lines derived from a cross of wild rice accession ACC10 and indica variety Zhenshan 97 (ZS97), we identified numerous quantitative trait loci for relative chlorophyll content. one major locus of them for relative chlorophyll content was mapped to a 10.3-kb region that contains OsGUX1. the allele OsGUX1 AC from ACC10 significantly decreases nitrogen content and chlorophyll content of leaf compared with OsGUX1 ZS from ZS97. The overexpression of OsGUX1 reduced chlorophyll content, and the suppression of this gene increased chlorophyll content of rice leaf. OsGUX1 is located in Golgi apparatus, and highly expressed in seedling leaf and the tissues in which primary cell wall synthesis occurring. our experimental data indicate that OsGUX1 is responsible for addition of glucuronic acid residues onto xylan and participates in accumulation of cellulose and hemicellulose in the cell wall deposition, thus thickening the primary cell wall of mesophyll cells, which might lead to reduced chlorophyll content in rice leaf. These findings provide insights into the association of cell wall components with leaf nitrogen content in rice.Rice (Oryza sativa. L) providing a staple food for more than half of the population in the world, is a monocot model species for genetics, biology and functional genomics studies. Leaf photosynthesis has great potential for the improvement of rice yield, which will significantly contribute to addressing food demand challenge. The chloroplast of green tissue or leaf, as the most important supporter of carbon fixation and energy transformation, plays important roles in photosynthesis 1-3 . Both genetic and environmental factors have effect on the biochemical composition in chloroplast, thus affecting photosynthetic rate 4 .Previous studies have indicated that leaf color is a sensitive indicator of crop growth, metabolism and nutritional status, and it is closely related to the content of photosynthetic pigment, and positively correlated with leaf nitrogen (N) content 5-7 . Understanding the genetic and physiological bases of leaf nitrogen status is essential for efficient crop production and nitrogen management in intensive rice cropping systems. Leaf greenness is determined by specific properties, such as leaf chlorophyll content and chloroplast development, and leaf morphological characteristics (leaf thickness, surface structure and wall components). To study the genetic and molecular basis of leaf color, more than one hundred of rice mutants associated with leaf colors and chlorophyll content have been identified (http://archive.gramene.org/db/genes/). Several genes have been implicated in the chlorophyl...

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“…It catalyzes the first step in CO 2 fixation to convert RuBP and CO 2 to two molecules of 3-phosphoglycerate (PGA). Thus, this enzyme activity directly affects the rate of CO 2 assimilation ( Darabi and Seddigh, 2017 ; Masumoto et al., 2015 ). RbcS and RbcL encode the large and small subunits of Rubisco, respectively, which work closely to regulate the structure and function of the Rubisco holoenzyme.…”

Section: Discussionmentioning

confidence: 99%

Exogenous selenium promotes the growth of salt-stressed tomato seedlings by regulating ionic homeostasis, activation energy allocation and CO2 assimilation

Zhang

Chen

et al. 2023

Front. Plant Sci.

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This study is aimed at investigating the effects of exogenous selenium (Se) on the ionic equilibrium and micro-domain distribution, state transitions between photosystem I (PSI) and photosystem II (PSII), and the photosynthetic carbon assimilation efficiency of tomato (Solanum lycopersicon L.) seedlings under the influence of salt stress. The application of 0.01 mmol•L-1 exogenous Se had no significant effects on the selective transport capacity of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) from the roots to leaves under salt stress. It, however, significantly hindered the absorption of Na by the root system and leaves, increased the ratios of K/Na, Ca/Na and Mg/Na, and relieved the nonuniformity of micro-domain ionic distribution, thus, mitigating the ionic homeostasis imbalance and ion toxicity induced by salt stress. Additionally, the application of exogenous Se overcame stomatal limitation, regulated the state transitions between PSI and PSII, and enhanced the initial and overall activity of Rubisco as well as the activities of Rubisco activase (RCA) and fructose-1,6-bisphosphatase (FBPase). It also increased the levels of expression of nine relevant genes in Calvin cycle, which subsequently improved the concentration of photosynthetic substrates, balanced the distribution of activation energy between PSI and PSII, promoted the efficiency of CO2 carboxylation and carbon assimilation, thereby increasing the photosynthetic efficiency of tomato seedling leaves under salt stress. Hence, the supply of exogenous Se can alleviate the inhibition of salt stress on tomato seedling growth by rebuilding ionic homeostasis and promoting photosynthetic capacity.

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Computational study of biochemical properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) enzyme in C3 plants

Cited by 8 publications

References 43 publications

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

A xylan glucuronosyltransferase gene exhibits pleiotropic effects on cellular composition and leaf development in rice

Exogenous selenium promotes the growth of salt-stressed tomato seedlings by regulating ionic homeostasis, activation energy allocation and CO2 assimilation

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