Computer‐Assisted Image Analysis of the Distribution and Intensity of Reactive Oxygen Species Accumulation in Plant Leaves

Sekulska‐Nalewajko, Joanna; Gocławski, Jarosław; Kużniak, Elźbieta

doi:10.1002/9781119468677.ch19

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…There are several example studies where the image processing method was used for the quantitative estimate of ROS accumulation in leaves. ImageJ has been used for determination of O 2 •− and H 2 O 2 level in leaves [ 41 , 42 , 43 , 65 ] and roots [ 66 ]. According to available data, O 2 •− and H 2 O 2 in seeds are visualized by staining with NBT or DAB, respectively.…”

Section: Discussionmentioning

confidence: 99%

Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology

Cembrowska-Lech

2020

IJMS

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The intracellular homeostasis of reactive oxygen species (ROS) and especially of superoxide anion and hydrogen peroxide participate in signaling cascades which dictate developmental processes and reactions to stresses. ROS are also biological molecules that play important roles in seed dormancy and germination. Because of their rapid reactivity, short half-life and low concentration, ROS are difficult to measure directly with high accuracy and precision. In presented work tissue printing method with image analysis and dual excitation flow cytometry (FCM) were developed for rapid detection and localization of O2•− and H2O2 in different part of seed. Tissue printing and FCM detection of ROS showed that germination of wild oat seeds was associated with the accumulation of O2•− and H2O2 in embryo (coleorhiza, radicle and scutellum), aleurone layer and coat. To verify if printing and FCM signals were specified, the detection of O2•− and H2O2 in seeds incubated in presence of O2•− generation inhibitor (DPI) or H2O2 scavenger (CAT) were examined. All results were a high level of agreement among the level of ROS derived from presented procedures with the ones created from spectrophotometric measured data. In view of the data obtained, tissue printing with image analysis and FCM are recommended as a simple and fast methods, which could help researchers to detection and level determination of ROS in the external and inner parts of the seeds.

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Section: Discussionmentioning

confidence: 99%

Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology

Cembrowska-Lech

2020

IJMS

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Modification of pigments content in red clover sprouts with the use of pulsed electric field

Gałązka-Czarnecka

Korzeniewska

Czarnecki

et al. 2021

J. Phys.: Conf. Ser.

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An innovative issue in food engineering is using the impulse electric field (PEF) in modeling the content of biologically active compounds in plant raw materials, including seeds. In the described experiment it was shown that it is possible to modify the carotenoid content in germinating seeds of red clover (Trifolium pratense L.) by treating the seeds with PEF before cultivation. The research material was the seeds of red clover ‘Rozeta’ variety. As a result of the conducted research, an increase in the content of lutein and zaaxanthin by 10% and β-carotene by 16% was obtained after the sprouts were subjected to a field of 5 kV/cm intensity.

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Computer‐Assisted Image Analysis of the Distribution and Intensity of Reactive Oxygen Species Accumulation in Plant Leaves

Cited by 2 publications

References 42 publications

Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology

Tissue Printing and Dual Excitation Flow Cytometry for Oxidative Stress—New Tools for Reactive Oxygen Species Research in Seed Biology

Modification of pigments content in red clover sprouts with the use of pulsed electric field

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