Computer assisted semen analysis for quantification of motion characteristics of bull sperm during cryopreservation cycle

Sundararaman, M.N.; Kalatharan, J.; Jawahar, K. Thilak Pon

doi:10.5455/vetworld.2012.723-726

Cited by 23 publications

(23 citation statements)

References 5 publications

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“…Also the results of the viability and concentration assessment of semen were correct and similar to those obtained by Garner et al (1997). Thanks to the technological possibilities of computerized semen analysis, detailed motility parameters were analyzed that corresponded to the results obtained by Contri et al (2010) and Sundararaman et al (2012).…”

Section: Discussionsupporting

confidence: 62%

Influence of the Degree of Ejaculate Dilution on the Results of the Assessment of Motility of Sperm of Breeding Bulls Obtained by Two Methods

Mizera¹,

Kuczaj²

2018

Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Z

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Streszczenie. Przeprowadzone doświadczenie miało na celu zbadania efektywności dwóch metod oceny parametrów ruchu plemników w nasieniu rozmrożonym, w zależności od stopnia jego rozrzedzenia. Nasienie pobrano od 12 buhajów rasy simentalskiej przy użyciu sztucznej pochwy. Próbki nasienia oceniono wstępnie pod względem objętości, barwy, pH, żywotności, koncentracji i ruchliwości, a następnie połączono w jedną próbę zbiorczą w celu eliminacji różnic indywidualnych, schłodzono do temperatury 4°C, zapakowano w słomki (0,25 ml) i zamrożono w ciekłym azocie po czym, przechowywano w kontenerze w temperaturze -196°C. Następnie rozmrożono, podzielono na próby A, B i C oraz rozrzedzono kolejno w stosunku 1 : 10 (próba A), 1 : 20 (próba B) i 1 : 30 (próba C); poddano ocenie wybrane parametry ruchu przy użyciu konwencjonalnej metody mikroskopowej oraz metody wspomaganej komputerowo (CASA). Uzyskane wyniki opracowano statystycznie. Całkowity ruch plemników analizowany dwiema metodami we wszystkich badanych próbach różnił się istotnie (p < 0,05). Ruch postępowy plemników badany w systemie CASA różnił się istotnie od badanego w przypadku użycia konwencjonalnej metody, przy rozrzedzeniu 1 : 20 oraz 1 : 30 (p < 0,05). Uzyskane wyniki ruchu postępowego przy rozrzedzeniu 1:20 z użyciem obu metod nie różniły się istotnie. Badania wykazały, że analiza ruchliwości przeprowadzona za pomocą systemu CASA cechowała się większą dokładnością i powtarzalnością wyników niż konwencjonalna metoda mikroskopowa. Ponadto wyniki poniższych badań wskazują na zależność pomiędzy stopniem rozrzedzenia nasienia a powtarzalnością wyników.

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Section: Discussionsupporting

confidence: 62%

Influence of the Degree of Ejaculate Dilution on the Results of the Assessment of Motility of Sperm of Breeding Bulls Obtained by Two Methods

Mizera¹,

Kuczaj²

2018

Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Z

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“…Adoption of CASA technique has been reported as the potential tool for improvements in evaluation of semen to enhance fertility (Sundararaman et al, 2012). Hence, it is of great interest to use a combination of semen motion characteristics which can predict bull of high fertility performance more accurately than a single test.…”

Section: Introductionmentioning

confidence: 99%

Post-thawed and fresh spermatozoa motion characteristics of Sahiwal bulls under computer-assisted semen analyser (CASA)

Galmessa¹,

Mohanty²,

Raina³

et al. 2014

Int. J. Livest. Prod.

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In this study, motion characteristics of spermatozoa was assessed by computer assisted semen analyser (CASA) for evaluating fertility potential of Sahiwal bulls. Twelve bulls were selected and grouped into two on the basis of age (AGI < 50 months old; AGII > 50 months old) and scrotal circumferences (SCI < 33 cm; SCII > 33 cm). The following CASA parameters i.e., velocity average path (VAP, μm/s), velocity straight line (VSL, μm/s), velocity curvilinear (VCL, μm/s), amplitude of lateral head displacement (ALH, μm), motility (%)(the percentage motile cells of the total) and straightness (STR) were recorded. Results of the study revealed that there is no significant different (p>0.05) in progressive motility either in age or Sc groups of bulls. However, significantly (p<0.05) higher mean post thaw motility was observed after 24 h cryopreservation for the younger (76.40±3.07) than the older (65.00±3.50) bulls and for larger SC than smaller SC bulls (65.56±3.78 vs. 56.56±3.78, p<0.05). Similar trends observed at 0 h after freezing were not significantly different (p>0.05) for both age and SC groups. In most motion characteristics especially in motility and linearity of the motion, younger bulls and bulls with larger SC performed better than older bulls and bulls with smaller SC indicating the possibility of selecting bulls at an early age on the basis of testis size to save the money, space and time which otherwise spent on rearing such inferior bulls. This study also clearly indicated that CASA is a good supplementation to aid for selection of breeding bulls.

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“…However, Keshav (1996) reported significantly lower (65.22%) mean motility for Sahiwal breed than the current study. On the other hand, a higher mean motility percentage of 94.3% was reported by Sundararaman et al (2012) in Jersey bulls. In chilled semen though significant difference was not observed between Cross and HF; significantly lower motility percentage (57.90%) was observed in Boran.…”

Section: Discussionmentioning

confidence: 76%

“…Though the total and progressive motility percentages of this study lies in the ranges of total and progressive motility percentages of Keshav (1996); her mean values were higher than this study finding. Sundararaman et al (2012) also reported a higher motility percentage for chilled (89.4%) and frozen (63.0%) semen in Jersey bulls. These possible variations between studies could be due to the reasons stated above for fresh semen.…”

Section: Discussionmentioning

confidence: 81%

Influence of breed on motility and motion characteristics of fresh, chilled and frozen bull spermatozoa

Seyoum¹,

Lemma²,

Tera³

2021

Int. J. Livest. Prod.

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At present, frozen-thawed semen is used extensively for artificial insemination (AI) in Ethiopia. However, subjective semen quality assessment is still practiced by the semen processing organizations of the country. In this study, motility and motion characteristics of spermatozoa were assessed by using integrated semen analysis system (ISAS) to diagnose breed differences in quality of semen. Semen was collected from 14 breeding bulls (Boran = 4, Crosses of 75 % Holstein Frisian ˟ 25% Boran = 4 and Holstein Frisian = 6). After initial subjective assessment, the semen was evaluated at fresh, chilled and frozen stages for various sperm motion characteristics using integrated semen analysis system (ISAS® v1, Proiser, Spain). PH, volume and morphological defects were differed significantly (P < 0.05) among breeds. Significantly higher (P < 0.05) motility percentage (82.5%) was identified in Boran fresh semen. In contrast spermatozoa motion characteristics: Medium, medium progressive and slow types were significantly higher (P < 0.05) in crosses. Individual motility percentage and spermatozoa motion types (medium and slow) were significantly low (P < 0.05) in Boran chilled semen. In line with chilled semen significantly high motility percentage (42.9%) for frozen semen was observed in HF. The sperm kinematic parameters: Average path velocity (VAP, µm/s), straight line velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), amplitude of lateral head displacement (ALH, µm), beat cross frequency (BCF, Hz) and straightness (STR) percentage were differed significantly (P < 0.05) among the three breeds at all stages of semen production. The smallest kinematic values of all parameters except for LIN, STR and WOB of fresh semen were recorded in cross breed bulls at all stages of production. On the other hand significantly higher (P < 0.05) values for all the parameters (VCL, VAP, VSL, ALH, BCF, LIN, STR and WOB) of frozen semen were recorded in Boran breed. Thus it is possible to conclude that breed has influence on motility and motion characteristics of bull spermatozoa at different stages of semen production.

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Computer assisted semen analysis for quantification of motion characteristics of bull sperm during cryopreservation cycle

Cited by 23 publications

References 5 publications

Influence of the Degree of Ejaculate Dilution on the Results of the Assessment of Motility of Sperm of Breeding Bulls Obtained by Two Methods

Influence of the Degree of Ejaculate Dilution on the Results of the Assessment of Motility of Sperm of Breeding Bulls Obtained by Two Methods

Post-thawed and fresh spermatozoa motion characteristics of Sahiwal bulls under computer-assisted semen analyser (CASA)

Influence of breed on motility and motion characteristics of fresh, chilled and frozen bull spermatozoa

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