Computer vision guided embryo biopsy

Abstract

Embryo biopsy, which is a process to remove a part of materials from a cell, is a necessary step of preimplantation genetic diagnosis (PGD). The rapid advancement of the PGD technique has been calling for easy, fast and precise automated embryo biopsy techniques to improve the efficiency, the throughput, and the survival rate of the PGD. In this thesis, a computer vision guided embryo biopsy system is introduced. This research is devoted to

Design and control of a piezo-driven flexure micromanipulator for the embryo biopsy

“…Schematic for chemical ZP drilling[40].Traditionally, as shown inFigure 2-9, embryos are stabilized by one holding pipette at 9 o'clock orientation, and another 10 µm drilling micropipette containing AT solution gets close to 3 o'clock orientation, closing to an area of empty perivitelline space. A 20~ 30 µm diameter hole is created by blowing the AT solution to the ZP area.…”