Concentrações e composições químicas do meio nutritivo para o cultivo in vitro de orquídea

Rodrigues, Donizetti Tomaz; Novais, Roberto Ferreira; V., Víctor Hugo Álvarez; Dias, José Maria Moreira

doi:10.1590/s0034-737x2012000100001

Cited by 7 publications

(6 citation statements)

References 8 publications

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“…The use of different formulations of media, with the addition of mineral salts, hormones, vitamins or organic compounds, is aimed at improving the germination and the asymbiotic in vitro development of these seedlings (Martini et al 2001;Campos 2004;Rodrigues et al 2012). However, in vitro experiments have shown that symbiotic seed germination enables faster development than does germination in asymbiotic culture media (Markovina & McGee 2000;Takahashi et al 2001;Stewart & Kane 2006).…”

Section: Introductionmentioning

confidence: 99%

Symbiotic propagation of seedlings of Cyrtopodium glutiniferum Raddi (Orchidaceae)

et al. 2013

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In nature, orchid seeds obtain the nutrients necessary for germination by degrading intracellular fungal structures formed after colonization of the embryo by mycorrhizal fungi. Protocols for asymbiotic germination of orchid seeds typically use media with high concentrations of soluble carbohydrate and minerals. However, when reintroduced into the field, seedlings obtained via asymbiotic germination have lower survival rates than do seedlings obtained via symbiotic germination. Tree fern fiber, the ideal substrate for orchid seedling acclimatization, is increasingly scarce. Here, we evaluated seed germination and protocorm development of Cyrtopodium glutiniferum Raddi cultivated in asymbiotic media (Knudson C and Murashige & Skoog) and in oatmeal agar (OA) medium inoculated with the mycorrhizal fungus Epulorhiza sp., using non-inoculated OA medium as a control. We also evaluated the performance of tree fern fiber, pine bark, eucalyptus bark, corncob and sawdust as substrates for the acclimatization of symbiotically propagated plants. We determined germination percentages, protocorm development and growth indices at 35 and 70 days of cultivation. Relative growth rates and the effects of substrates on mycorrhizal formation were calculated after 165 days of cultivation. Germination efficiency and growth indices were best when inoculated OA medium was used. Corncob and pine bark showed the highest percentages of colonized system roots. The OA medium inoculated with Epulorhiza sp. shows potential for C. glutiniferum seedling production. Corncob and pine bark are promising substitutes for tree fern fiber as substrates for the acclimatization of orchid seedlings.

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Section: Introductionmentioning

confidence: 99%

Symbiotic propagation of seedlings of Cyrtopodium glutiniferum Raddi (Orchidaceae)

et al. 2013

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“…2ml were inoculated in the alternative culture media consisting of 30 g L -1 of commercial sugar, 2 g L -1 of fertilizer B & G  , 200 ml L -1 of coconut water, and 2 g L -1 of activated carbon. They were de-jellied with 4 g L -1 of Agar (Rodrigues et al, 2012) and the pH of the culture media was adjusted to 5.5 ± 0.05.…”

Section: Methodsmentioning

confidence: 99%

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

et al. 2017

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The aim of this study was to compare vitroplants Catasetum x apolloi grown under natural light and artificial light and different concentrations of potassium silicate, providing data on the anatomical differentiation that aids the acclimatization process of this species. Plants from in vitro seeding were used; 5 protocorms of approximately 0.5 cm were inoculated into vials with a capacity of 500 mL containing 100 mL of alternative culture medium plus potassium silicate (0.0, 0.5; 1.0 mL L -1 ), pH adjusted to 5.5 ± 0.5 and gelated with 4GL -1 agar before the autoclaving process. Cultures were maintained under natural light (TNE) and artificial light (TAE) for 90 days, and micromorphometric analysis was performed for polar and equatorial diameter, density and stomatal index, blade thickness in the central rib, and secondary veins. Applications in K 2 SiO 4 alternative medium provided the following: elongation of the hypodermis, thicker mesophyll, and more prominent midrib; elipptical guard cells; formation of epistomatal chamber; and lower stomatal density and stomatal with lower equatorial and polar diameters. The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses.Keywords: leaf anatomy, light, orchids, plants vitro. Anatomia e análise micromorfométrica foliar de Catasetum x apolloi Benelli & Grade com adição de silicato de potássio em diferentes fontes de luzResumo O objetivo desse trabalho foi comparar vitroplantas de Catasetum x apolloi cultivadas sob luz natural e luz artificial e diferentes concentrações de silicato de potássio, fornecendo dados sobre diferenciação anatômica que auxiliem no processo de aclimatação dessa espécie. Utilizou-se plantas provenientes da semeadura in vitro, 5 protocormos de aproximadamente 0,5 cm foram inoculados em frascos com capacidade para 500 mL contendo 100 mL de meio de cultura alternativo, acrescido de silicato de potássio (0,0; 0,5; 1,0 mL L -1 ), pH ajustado para 5,5 ±0,5 e gelificado com 4gL -1 de ágar antes do processo de autoclavagem. As culturas foram mantidas sob luz natural (TAA) e luz artificial (TAN) por 90 dias, e feitas análises micromorfométricas (diâmetro polar e equatorial, densidade e índice estomático, espessura do limbo na nervura central e nervuras secundárias). As aplicações de K 2 SiO 4 em meio alternativo, propiciaram: alongamento da hipoderme; mesofilo mais espesso e nervura central mais proeminente; células guardas elípticas; formação de câmaras supraestomáticas; menor densidade estomática e estômatos com menores diâmetros equatorial e polar. As condições que podem favorecer a aclimatação são menores intensidades de luz e menores doses de silicato de potássio.Palavras-chave: anatomia foliar, luminosidade, orquídeas, vitroplantas.

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“…Em experimento anterior, doses de fertilizantes minerais compreendidas entre 0,25 e 2,25 g L -1 resultaram, para muitas variáveis de crescimento, em curvas lineares, não sendo possível definir a melhor dose de fertilizante a ser adicionada ao meio de cultivo (Rodrigues et al, 2012). Com base nesses resultados, foram estabelecidas as doses para este experimento.…”

Section: Resultsunclassified

Cultivo in vitro de plântulas de orquídea em meios com diferentes concentrações de fertilizante mineral

et al. 2012

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In vitro cultivation of orchid seedlings in media with differents concentrations of mineral fertilizerThere are a number of medium formulations for growth of orchid seedlings in vitro, with different compositions and concentrations of salts, leading to varying plant responses to them. The objective of this work was to evaluate in vitro growth of orchid (Cattleya walkeriana Gardner) seedlings subjected to different rates of a NPK fertilizer added to the growth medium. The fertilizer Peters ® was used in the formulation NPK 10-30-20+Mg+micronutrients in the rates of 0.0; 1.0; 2.0; 3.0; 4.0; 5.0; and 10.0 g L -1 medium, with coconut milk (150 mL L -1 ), sucrose (20 g L -1 ) and activated charcoal (2 g L -1 ) and pH adjusted to 5.6. Six-month-old seedlings, previously germinated in Knudson C medium were transferred to 320 mL glass jars containing 35 mL of medium, using 10 plants per jar. The maximum production of dry matter from the aerial part at six months of age was estimated at 0.255 g/jar, with the medium added of 5.22 g L -1 of fertilizer. Considering the possibility of high salt concentration in the medium for some orchid species, the fertilizer rate needed to obtain 90% of maximum production was estimate at 3.55 g L -1 . There was a drastic growth reduction in root growth when using high fertilizer concentrations, producing fewer roots and shorter.

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Concentrações e composições químicas do meio nutritivo para o cultivo in vitro de orquídea

Cited by 7 publications

References 8 publications

Symbiotic propagation of seedlings of Cyrtopodium glutiniferum Raddi (Orchidaceae)

Symbiotic propagation of seedlings of Cyrtopodium glutiniferum Raddi (Orchidaceae)

Anatomy and micromorphometric analysis of leaf Catasetum x apolloi Benelli & Grade with addition of potassium silicate under different light sources

Cultivo in vitro de plântulas de orquídea em meios com diferentes concentrações de fertilizante mineral

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