Concentrating Single Cells in Picoliter Droplets for Phospholipid Profiling on a Microfluidic System

Zhang, Weifei; Li, Nan; Lin, Ling; Huang, Qin; Uchiyama, Katsumi; Lin, Jin‐Ming

doi:10.1002/smll.201903402

Cited by 43 publications

(31 citation statements)

References 46 publications

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“…In addition, DEP techniques are also used to control the position of cells in microdroplets. 163 The size of microdroplets was further decreased by the shear of a bifurcated channel for reducing the reaction volume and matrix interference with cell detection. Currently, a commercial DEP-based chip named DEP Array has been used to control cells to form the single-cell array.…”

Section: Dielectrophoresismentioning

confidence: 99%

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Dong

Wang

Yin

et al. 2022

VIEW

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Gene heterogeneity leads to the differences in cellular behaviors in a wide range, such as tumor drug‐resistant mutation, epithelial‐mesenchymal transition, and migration, posing significant challenges to the development of biomedicine. Traditional gene analysis methods, such as polymerase chain reaction, employ a mass of cells as the gene source, resulting in that the gene properties from a specific single cell are hidden in massive gene information. Recent decades have seen the emerging single‐cell gene analysis techniques with their unprecedented opportunities to study gene heterogeneity with high precision and high throughput. In this review, we summarized the state‐of‐the‐art techniques for single‐cell sequencing and on‐chip gene analysis systems. The principles of each technique are introduced in detail, with the focus on the application scenarios in gene heterogeneity research. Looking forward, we also introduced the challenges in current technologies and point out the future direction for facilitating the technical improvement and clinical applications of single‐cell gene analysis techniques.

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Section: Dielectrophoresismentioning

confidence: 99%

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Dong

Wang

Yin

et al. 2022

VIEW

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“…This system could provide the physiological context for the live single cell with well-preserved lipid information. Using the system, the heterogeneities between the normal cells and cancer cells were caught, meanwhile, the heterogeneity of the same cells before and after the drug treatment changed obviously [119].…”

Section: Hydrogel Droplet For Biomedical Cell Encapsulation and Analysismentioning

confidence: 99%

The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

Song

et al. 2020

Micromachines

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Microfluidic systems have been widely explored based on microfluidic technology, and it has been widely used for biomedical screening. The key parts are the fabrication of the base scaffold, the construction of the matrix environment in the 3D system, and the application mechanism. In recent years, a variety of new materials have emerged, meanwhile, some new technologies have been developed. In this review, we highlight the properties of high throughput and the biomedical application of the microfluidic chip and focus on the recent progress of the fabrication and application mechanism. The emergence of various biocompatible materials has provided more available raw materials for microfluidic chips. The material is not confined to polydimethylsiloxane (PDMS) and the extracellular microenvironment is not limited by a natural matrix. The mechanism is also developed in diverse ways, including its special physical structure and external field effects, such as dielectrophoresis, magnetophoresis, and acoustophoresis. Furthermore, the cell/organ-based microfluidic system provides a new platform for drug screening due to imitating the anatomic and physiologic properties in vivo. Although microfluidic technology is currently mostly in the laboratory stage, it has great potential for commercial applications in the future.

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“…Zhang et al developed a high automatic platform for single-cell analysis, which consists of droplet-based inkjet printing, dielectrophoretic electrodes, and de-emulsification interface to achieve on-line single-cell encapsulation, manipulation, and MS detection. 67 As shown in Fig. 7, the cell culture medium is removed by droplet cutting, and the entire cell is injected to MS for detection, and information about phospholipids is obtained.…”

Section: Analytical Sciencesmentioning

confidence: 99%

Cell Analysis on Microfluidics Combined with Mass Spectrometry

Zhang

Lin

2020

ANAL. SCI.

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Cell analysis is of great significance for the exploration of human diseases and health. However, there are not many techniques for high-throughput cell analysis in the simulated cell microenvironment. The high designability of the microfluidic chip enables multiple kinds of cells to be co-cultured on the chip, with other functions such as sample preprocessing and cell manipulation. Mass spectrometer (MS) can detect a large number of biomolecules without labelling. Therefore, microfluidic chip coupled with MS has been a major branch of cell analysis during the past decades. Here, we concisely introduce various microfluidic devices coupling with MS used for cell analysis. The main functions of microfluidic devices were first described, followed with different interfaces to different types of MS. Then, their various applications in cell analysis were highlighted, with an emphasis on cell metabolism, drug screening, and signal transduction. Current limitations and prospective trends of microfluidics coupling with MS are discussed at the end.

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Concentrating Single Cells in Picoliter Droplets for Phospholipid Profiling on a Microfluidic System

Cited by 43 publications

References 46 publications

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

The Fabrication and Application Mechanism of Microfluidic Systems for High Throughput Biomedical Screening: A Review

Cell Analysis on Microfluidics Combined with Mass Spectrometry

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