Concentration and Time Effects of Dextran Exposure on Endothelial Cell Viability, Attachment, and Inflammatory Marker Expression In Vitro

Rouleau, Léonie; Rossi, John; Leask, Richard L.

doi:10.1007/s10439-010-9934-4

Cited by 34 publications

(24 citation statements)

References 52 publications

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“…Dex has excellent biocompatibility, and has been used in clinics for a long time, but modification of cell-cell interaction and cell viability has also been observed. 31 However, our data found no impact on viability and autophagy of cells by Dex. These results indicated that these NPs had good biocompatibility.…”

Section: Discussioncontrasting

confidence: 71%

Iron oxide nanoparticles and induced autophagy in human monocytes

Jin²,

Feng

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles have been widely used in biomedical applications, but understanding of their interactions with the biological immune system is relatively limited. This work is focused on dextran-coated iron oxide nanoparticles and their induced autophagy in human monocytes. We found that these nanoparticles can be taken up by human monocytes, followed by localization within vesicles or free in cytoplasm. Autophagosome formation was observed with increased expression of LC3II protein, the specific marker of autophagy. The autophagy substrate p62 was degraded in a dose-dependent manner, and autophagy was blocked by autophagy (or lysosome) inhibitors alone or along with iron oxide nanoparticles, indicating that autophagosome accumulation was mainly due to autophagy induction, rather than blockade of autophagy flux. Interestingly, iron oxide nanoparticles increased the viability of human monocytes, but the mechanism was not clear. We further found that inhibition of autophagy mostly attenuated the survival of cells, with acceleration of the inflammation induced by these nanoparticles. Taken together, autophagic activation in human monocytes may play a protective role against the cytotoxicity of iron oxide nanoparticles.

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Section: Discussioncontrasting

confidence: 71%

Iron oxide nanoparticles and induced autophagy in human monocytes

Jin²,

Feng

et al. 2017

IJN

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“…The calibrated spacers used as cone supports provided a simple and accurate method for positioning the cone and modulating the gap h 0 between the cone apex and the plate surface. The addition of high molecular weight dextran to cell culture medium, allows us to investigate a wide range of WSS magnitudes ensuring the onset of primary, laminar flow without compromising cell biology (Rouleau et al 2010). Even at the highest cone rotation velocity, limited to 20 rad/s for safety reasons, the modified Reynolds number remains lower than 0.5, and thus negligible secondary flows develop (Sdougos et al 1984).…”

Section: Discussionmentioning

confidence: 99%

Design of a cone-and-plate device for controlled realistic shear stress stimulation on endothelial cell monolayers

et al. 2016

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Endothelial cells are constantly exposed to blood flow and the resulting frictional force, the wall shear stress, varies in magnitude and direction with time, depending on vasculature geometry. Previous studies have shown that the structure and function of endothelial cells, and ultimately of the vessel wall, are deeply affected by the nature of wall shear stress waveforms. To investigate the in vitro effects of these stimuli, we developed a compact, programmable, realtime operated system based on cone-and-plate geometry, that can be used within a standard cell incubator. To verify the capability to replicate realistic shear stress waveforms, we calculated both analytically and numerically to what extent the system is able to correctly deliver the stimuli defined by the user at plate level. Our results indicate that for radii greater than 25 mm, the shear stress is almost uniform and directly proportional to cone rotation velocity. We further established that using a threshold of 10 Hz of wall shear stress waveform frequency components, oscillating flow conditions can be reproduced on cell monolayer surface. Finally, we verified the capability of the system to perform long-term flow exposure experiments ensuring sterility and cell culture viability on human umbilical vein endothelial cells exposed to unidirectional and oscillating shear stress. In conclusion, the system we developed is a highly dynamic, easy to handle, and able to generate pulsatile and unsteady oscillating wall shear stress waveforms. This system can be used to investigate the effects of realistic stimulations on endothelial cells, similar to those exerted in vivo by blood flow.

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“…1. The viscosity of the cell culture medium was measured to be 0.975 cP at 37°C using a double gap rheometer (Bohlin, Model CVO 120 h) (Rouleau et al 2010b). From the measured viscosity and model radius, the following equation was used to calculate the volumetric flow rate (Q) required to obtain a desired wall shear stress in a straight rigid tube.…”

Section: Flow Experimentsmentioning

confidence: 99%