Concentration dependence of the cell membrane permeability to cryoprotectant and water and implications for design of methods for post-thaw washing of human erythrocytes

Lahmann, John M.; Benson, James D.; Higgins, Adam Z.

doi:10.1016/j.cryobiol.2017.12.003

Cited by 27 publications

(17 citation statements)

References 85 publications

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“…Our results demonstrate that the water permeability of bovine oocytes decreases as the CPA concentration increases. This trend has been observed previously for various other cell types 25 , 39 , 40 , and has been explained in terms of steric hindrance caused by CPA binding to water-transporting channels 41 . Bovine oocytes are known to express aquaporins 36 , 42 and the observation that water permeability decreases as CPA concentration increases is consistent with such a mechanism.…”

Section: Discussionsupporting

confidence: 85%

“…Permeability parameters allow calculation of cellular osmotic responses during the addition and removal of CPA, and can provide evidence for whether water and solute movement occurs through channels or by simple diffusion through the lipid bilayer 24 . Recently, it has been shown that the permeability of the erythrocyte cell membrane to water and CPA is dependent on concentration 25 , contrary to what previous studies have assumed 26 .…”

Section: Introductionmentioning

confidence: 65%

“…To account for the effects of CPA concentration on water and CPA permeability, we fit the permeability data to a concentration-dependent model 25 . This model is consistent with a transport mechanism that is limited by binding of CPA to a transporter protein such as an aquaporin.…”

Section: Resultsmentioning

confidence: 99%

“…Typically cell membrane permeability parameters are determined for exposure to a single CPA concentration 3 , 6 , 36 . However, oocytes are exposed to various CPA concentrations during the vitrification process, and previous studies suggest that the water and CPA permeability may be concentration dependent 25 . Therefore, in this study, we examined the potential effects of CPA concentration on the membrane permeability parameters and the implications for bovine oocyte vitrification.…”

Section: Discussionmentioning

confidence: 99%

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Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches

García-Martínez

Mogas

Mullen

et al. 2021

Sci Rep

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The plasma membrane permeability to water and cryoprotectant (CPA) significantly impacts vitrification efficiency of bovine oocytes. Our study was designed to determine the concentration-dependent permeability characteristics for immature (GV) and mature (MII) bovine oocytes in the presence of ethylene glycol (EG) and dimethyl sulphoxide (Me2SO), and to compare two different modeling approaches: the two parameter (2P) model and a nondilute transport model. Membrane permeability parameters were determined by consecutively exposing oocytes to increasing concentrations of Me2SO or EG. Higher water permeability was observed for MII oocytes than GV oocytes in the presence of both Me2SO and EG, and in all cases the water permeability was observed to decrease as CPA concentration increased. At high CPA concentrations, the CPA permeability was similar for Me2SO and EG, for both MII and GV oocytes, but at low concentrations the EG permeability of GV oocytes was substantially higher. Predictions of cell volume changes during CPA addition and removal indicate that accounting for the concentration dependence of permeability only has a modest effect, but there were substantial differences between the 2P model and the nondilute model during CPA removal, which may have implications for design of improved methods for bovine oocyte vitrification.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches

García-Martínez

Mogas

Mullen

et al. 2021

Sci Rep

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“…By contrast, glycerol is transported in sheep RBC via simple diffusion only (41,42), and hence it has a lower permeability coe cient compared to humans (43). In humans, the effects of glycerol permeation on RBC functionality have been described in in vitro studies focusing on RBC cryopreservation, where glycerol is used as penetrating cryoprotectant (44). It has been reported that glycerol permeation in the RBC membrane resulted in shape alterations (increased MCV and RDW-SD) which appeared to be closely related to membrane alterations, increased permeability to ions and increased osmotic fragility (45).…”

Section: Discussionmentioning

confidence: 99%

Administration of Glycerol-Based Formulations in Sheep Results in Similar Ovulation Rate to eCG but Red Blood Cell Indices May Be Affected

Porcu

Sotgiu

Pasciu

et al. 2020

Preprint

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Background: The objective of this study was to investigate the metabolic and osmotic effects of different doses of glycerol or a glycerol – propylene glycol mixture in Sarda sheep with the aim to identify those able to beneficially modify ewe’s metabolic status without harmful changes in red blood cell (RBC) indices. Thereafter, the selected doses were tested for their effects on ewe’s ovarian activity during an induced follicular phase and compared to the effects of a hormonal treatment with equine chorionic gonadotrophin (eCG). Results: Glycerol was administered alone (G groups: 90% glycerol and 10% water; % v/v) or in combination with propylene glycol (M groups: 70% glycerol, 20% propylene glycol, 10% water; % v/v). Treatments were formulated to provide 100%, 75%, 50% and 25% of the amount of energy supplied in previous experiments. Obtained results showed that the formulations G75 and M75 (22.5% and 18.2% on DM basis, respectively) induce metabolic changes comparable to those induced by M100. The latter dose has been already evaluated for its effects on sheep metabolism and reproductive performance. However, with these high doses, plasma osmolality increased significantly, and RBC indices showed significant alterations. The low dose groups (G25 and M25, 8.6% and 6.9% on DM basis, respectively) did not show any alterations in plasma osmolality and RBC indices, but the metabolic milieu differed markedly from that of M100. Between the medium dose groups, M50 (12.9% on DM basis) showed a more comparable milieu to M100 than G50 (15.9% on DM basis) and no RBC alterations. Therefore, M75, G75 and M50 doses were tested for their effect on ovarian functions and proved to be equally effective as eCG. Conclusion: The results of the present study evidenced an alteration of RBC indices, and possibly of their functions, as a side effect of glycerol administration at high doses in the diet of ewes. Therefore, protocols foreseeing the administration of glycerol should be tested for their effects on RBC indices and functions. In general terms, the medium dose of the glucogenic mixture (12.9% of dietary DM on offer) should be preferred.

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Cryopreservation of organoids: Strategies, innovation, and future prospects

Han,

Zhan,

Guo

et al. 2024

Biotechnology Journal

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Organoid technology has demonstrated unique advantages in multidisciplinary fields such as disease research, tumor drug sensitivity, clinical immunity, drug toxicology, and regenerative medicine. It will become the most promising research tool in translational research. However, the long preparation time of organoids and the lack of high‐quality cryopreservation methods limit the further application of organoids. Although the high‐quality cryopreservation of small‐volume biological samples such as cells and embryos has been successfully achieved, the existing cryopreservation methods for organoids still face many bottlenecks. In recent years, with the development of materials science, cryobiology, and interdisciplinary research, many new materials and methods have been applied to cryopreservation. Several new cryopreservation methods have emerged, such as cryoprotectants (CPAs) of natural origin, ice‐controlled biomaterials, and rapid rewarming methods. The introduction of these technologies has expanded the research scope of cryopreservation of organoids, provided new approaches and methods for cryopreservation of organoids, and is expected to break through the current technical bottleneck of cryopreservation of organoids. This paper reviews the progress of cryopreservation of organoids in recent years from three aspects: damage factors of cryopreservation of organoids, new protective agents and loading methods, and new technologies of cryopreservation and rewarming.

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Concentration dependence of the cell membrane permeability to cryoprotectant and water and implications for design of methods for post-thaw washing of human erythrocytes

Cited by 27 publications

References 85 publications

Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches

Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches

Administration of Glycerol-Based Formulations in Sheep Results in Similar Ovulation Rate to eCG but Red Blood Cell Indices May Be Affected

Cryopreservation of organoids: Strategies, innovation, and future prospects

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