Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Purpose: To investigate the effect of Scutellaria barbata D. Don extract (SBDE) on apoptosis and proliferation in A549 human lung cancer cells. Methods: Inverted microscope was used to observe morphological changes in A549 cells after exposure to SBDE. Trypan blue staining of living cells was applied to construct cell growth curve after treatment with varying concentrations of SBDE. The influence of SBDE on cell proliferation, apoptosis and cell cycle was determined by MTT assay while protein expressions of key apoptosis-related enzymes were evaluated by immuno-cytochemical method. Results: SBDE inhibited the growth of A549 lung cancer cells at a concentration range of 20 - 160 μg/mL. Flow cytometry showed that SBDE induced apoptosis in the A549 cells. The proportion of cells in G0/G1-phase increased significantly (p < 0.01), while the proportion of cells in S-phase and G2/Mphase decreased correspondingly, indicating that the cells were in G0/G1-phase arrest. Cell cycle arrest and apoptosis-inducing effect gradually increased with increase in SBDE concentration. With increasing concentrations of SBDE, there were significant increases in the expressions of caspase-3 (p < 0.05), caspase-8 (p < 0.01) and caspase-9 (p < 0.05), and significant decreases in Ki-67 (p < 0.01) and p21 ras protein (p < 0.01). Conclusion: SBDE exerts significant inhibitory effect on the proliferation of A549 lung cancer cells, which can be developed for the treatment of lung cancer patients.

Section: Discussionmentioning

confidence: 99%

Anti-proliferation effect of Scutellaria barbata D. Don extract on lung cancer

Hu¹,

Duan²

2021

“…Ki-67 antigen is a more positive marker of nuclear proliferation, and Ki-67 may be used as a biomarker for determining high risk individuals in precancer population [7][8][9]. Results from Ki-67 antigen assay showed that PPE significantly decreased the expression of Ki-67 in A549 cells.…”

Section: Discussionmentioning

confidence: 99%

Paris polyphylla extract inhibits proliferation and promotes apoptosis in A549 lung cancer cells

Hu¹,

Yu²,

Zhuo³

et al. 2018

“…Ki-67 antigen is a more positive marker of nuclear proliferation, and Ki-67 may be used as a biomarker for determining high risk individuals in pre-cancer population [7][8][9]. Results from Ki-67 antigen assay showed that CNCE significantly decreased the expression of Ki-67 in A549 cells.…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferation and apoptosis effects of <i>Camellia nitidissima</i> C. W. Chi extract on A549 lung cancer cells

Zhang¹,

Li²

2018

To investigate the effect of Camellia nitidissima C. W. Chi extract (CNCE) on apoptosis and proliferation in A549 human lung cancer cells. Methods: Inverted microscope was used to examine morphological changes in A549 cells after exposure to CNCE. Trypan blue staining of living cells was applied to construct the cell growth curve after treatment with varying concentrations of CNCE. The influence of CNCE on cell proliferation, apoptosis and cell cycle were determined by MTT assay. The protein expressions of key apoptosisrelated enzymes were evaluated by immuno-cytochemical method. Results: CNCE inhibited the growth of A549 lung cancer cells at the concentration range of 20-160 μg/mL. Flow cytometry showed that CNCE induced apoptosis in the A549 cells. The proportion of cells in G0/G1-phase increased significantly (p < 0.01), while the proportion of cells in S-phase and G2/Mphase decreased correspondingly, indicating that the cells were in G0/G1-phase arrest. Cell cycle arrest and apoptosis-inducing effect gradually increased with increase in CNCE concentration. With increasing concentrations of CNCE, there were significant increases in the expressions of caspase-3 (p < 0.05), caspase-8 (p < 0.01) and caspase-9 (p < 0.05), and significant decreases in Ki-67 (p < 0.01) and p21 ras protein (p < 0.01). Conclusion: CNCE exerts significant inhibitory effect on the proliferation of A549 lung cancer cells, and therefore can potentially be developed for the treatment of lung cancer.