Concept Note: Toward Metagenomic Sequencing for Rapid, Sensitive, and Accurate Detection of Bacterial Pathogens in Potato Seed Production

Román-Reyna, Verónica; Rioux, Renee A.; Babler, Brooke; Klass, Taylor L.; Jacobs, Jonathan M.

doi:10.1094/phytofr-04-22-0037-fi

Cited by 5 publications

(1 citation statement)

References 33 publications

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“…Therefore, new methods based, for example, on immunosensors [122] or biosensors targeted for usage in the field have been created [123]. More sophisticated methods like metagenomic sequencing can be applied in seed production [124]. The early detection of SRPs can be assisted by automated image detection techniques [125,126].…”

Section: Soft Rot Pectobacteriaceae Detectionmentioning

confidence: 99%

Looking for Resistance to Soft Rot Disease of Potatoes Facing Environmental Hypoxia

Maciag,

Kozieł,

Otulak-Kozieł

et al. 2024

IJMS

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Plants are exposed to various stressors, including pathogens, requiring specific environmental conditions to provoke/induce plant disease. This phenomenon is called the “disease triangle” and is directly connected with a particular plant–pathogen interaction. Only a virulent pathogen interacting with a susceptible plant cultivar will lead to disease under specific environmental conditions. This may seem difficult to accomplish, but soft rot Pectobacteriaceae (SRPs) is a group virulent of pathogenic bacteria with a broad host range. Additionally, waterlogging (and, resulting from it, hypoxia), which is becoming a frequent problem in farming, is a favoring condition for this group of pathogens. Waterlogging by itself is an important source of abiotic stress for plants due to lowered gas exchange. Therefore, plants have evolved an ethylene-based system for hypoxia sensing. Plant response is coordinated by hormonal changes which induce metabolic and physiological adjustment to the environmental conditions. Wetland species such as rice (Oryza sativa L.), and bittersweet nightshade (Solanum dulcamara L.) have developed adaptations enabling them to withstand prolonged periods of decreased oxygen availability. On the other hand, potato (Solanum tuberosum L.), although able to sense and response to hypoxia, is sensitive to this environmental stress. This situation is exploited by SRPs which in response to hypoxia induce the production of virulence factors with the use of cyclic diguanylate (c-di-GMP). Potato tubers in turn reduce their defenses to preserve energy to prevent the negative effects of reactive oxygen species and acidification, making them prone to soft rot disease. To reduce the losses caused by the soft rot disease we need sensitive and reliable methods for the detection of the pathogens, to isolate infected plant material. However, due to the high prevalence of SRPs in the environment, we also need to create new potato varieties more resistant to the disease. To reach that goal, we can look to wild potatoes and other Solanum species for mechanisms of resistance to waterlogging. Potato resistance can also be aided by beneficial microorganisms which can induce the plant’s natural defenses to bacterial infections but also waterlogging. However, most of the known plant-beneficial microorganisms suffer from hypoxia and can be outcompeted by plant pathogens. Therefore, it is important to look for microorganisms that can withstand hypoxia or alleviate its effects on the plant, e.g., by improving soil structure. Therefore, this review aims to present crucial elements of potato response to hypoxia and SRP infection and future outlooks for the prevention of soft rot disease considering the influence of environmental conditions.

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Section: Soft Rot Pectobacteriaceae Detectionmentioning

confidence: 99%

Looking for Resistance to Soft Rot Disease of Potatoes Facing Environmental Hypoxia

Maciag,

Kozieł,

Otulak-Kozieł

et al. 2024

IJMS

View full text Add to dashboard Cite

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Application of molecular methods for potato disease diagnosis: a review

Jiang,

Feindel,

Harding

et al. 2024

Canadian Journal of Plant Pathology

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Mitigating Emerging and Reemerging Diseases of Fruit and Vegetable Crops in a Changing Climate

Miller,

Testen,

Jacobs

et al. 2024

Phytopathology®

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Fruit and vegetable crops are important sources of nutrition and income globally. Producing these high value crops requires significant investment of often scarce resources, and therefore the risks associated with climate change and accompanying disease pressures are especially important. Climate change influences the occurrence and pressure of plant diseases, enabling new pathogens to emerge and old enemies to re-emerge. Specific environmental changes attributed to climate change, particularly temperature fluctuations and intense rainfall events, greatly alter fruit and vegetable disease incidence and severity. In turn, fruit and vegetable microbiomes, and subsequently overall plant health, are also affected by climate change. Changing disease pressures cause growers and researchers to reassess disease management and climate change adaptation strategies. Approaches such as climate smart IPM, smart sprayer technology, protected culture cultivation, advanced diagnostics, and new soilborne disease management strategies are providing new tools for specialty crops growers. Researchers and educators need to work closely with growers to establish fruit and vegetable production systems that are resilient and responsive to changing climates. This review explores the effects of climate change on specialty food crops, pathogens, insect vectors, and pathosystems, as well as adaptations needed to insure optimal plant health and environmental and economic sustainability.

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Concept Note: Toward Metagenomic Sequencing for Rapid, Sensitive, and Accurate Detection of Bacterial Pathogens in Potato Seed Production

Cited by 5 publications

References 33 publications

Looking for Resistance to Soft Rot Disease of Potatoes Facing Environmental Hypoxia

Looking for Resistance to Soft Rot Disease of Potatoes Facing Environmental Hypoxia

Application of molecular methods for potato disease diagnosis: a review

Mitigating Emerging and Reemerging Diseases of Fruit and Vegetable Crops in a Changing Climate

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