Purification of wastewater from petroleum products is an urgent problem for the Tyumen province as an oil and gas region. The use of natural materials is actively studied in the technologies of sorption cleaning which is the main method for oil pollution removal. One of these new technologies is the wastewater treatment method with the use of probiotic agents. The purpose of this research is to study the effect of probiotics on the degree of dissolved petroleum products extraction from aqueous solutions by phytosorbent (pine sawdust). The photometric method of liquid analysis was used as a standard method of research. As a result of the sorption process study, it was found that the concentration of petroleum products decreased faster and to a minimum value (by 48.68%) when filtering samples through a layer of sawdust soaked in a probiotic solution, which is 2 times more effective than pure sawdust. The sorbent used is environmentally friendly.