Conchotome and needle percutaneous biopsy of skeletal muscle.

Dietrichson, P; Coakley, J. H.; Smith, Philip E. M.; Griffiths, Richard D.; Helliwell, Tim; Edwards, R. H. T.

doi:10.1136/jnnp.50.11.1461

Cited by 140 publications

(93 citation statements)

References 17 publications

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“…Under local anaesthesia, biopsies were taken from anterior tibialis muscle, using a conchotome [14]. Samples used for the determination of parameters of muscle energy metabolism were immediately frozen in liquid nitrogen and stored at -808C.…”

Section: Collection and Analysis Of Muscle Biopsiesmentioning

confidence: 99%

Muscle metabolic status in patients with severe COPD with and without long-term prednisolone

Pouw¹,

Lang²,

Gosker³

et al. 2000

Eur Respir J

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Both abnormalities in high energy phosphate metabolism and a decreased oxidative enzyme capacity have been reported in skeletal muscle of stable chronic obstructive pulmonary disease (COPD) patients. The first aim of this study was to investigate whether these findings are present in anterior tibialis muscle and whether or not they are associated. Abnormalities in mitochondrial structure and function as well as signs of myopathy have been found during corticosteroid treatment. The second aim of this study, therefore, was to investigate whether in COPD patients prolonged use of low dose prednisolone has effects on muscle energy metabolism and qualitative morphology.In a cross-sectional study 15 COPD patients (forced expiratory volume in one second (FEV1) 33 9 (mean SD) % predicted) who were steroid-naive (CORT-) were compared with 10 healthy control subjects (HC) and with 14 COPD patients (FEV1 30 11 % pred), who had been using oral prednisolone for at least 1 yr (CORT+).It was found that adenosine triphosphate (ATP)/adenosine diphosphate was lower in CORT-compared to HC (5.7 versus 6.2, p=0.03). Inosine monophosphate was detected in 13 of 15 CORT-compared to 3 of 10 HC (p=0.004). However, although indications were found for an imbalance in production and utilization of ATP, comparing CORT-and HC, no differences in oxidative (citrate synthase and 3-hydroxy-acylcoenzyme A dehydrogenase) and glycolytic (hexokinase, lactate dehydrogenase and phosphofructokinase) enzyme capacities were found.When, comparing steroid-treated and steroid-naive patient subgroups, no differences in the above mentioned parameters of muscle energy metabolism and of muscle qualitative morphology were found. Eur Respir J 2000; 16: 247±252. Supported by a scholarship from ASTRA BV, the Netherlands.Exercise intolerance and dyspnoea are the most frequently occurring complaints in patients with severe chronic obstructive pulmonary disease (COPD). Weakness of both skeletal and respiratory muscles contributes significantly to these complaints [1]. Recently it has been shown that muscle weakness in COPD patients can predominantly be explained by muscle atrophy [2]. However, several studies suggested that exercise tolerance might also be impaired by alterations in muscle energy metabolism [3±5].Experimental evidence is accumulating that in COPD patients muscle energy metabolism is already disturbed at rest. JAKOBSSON et al. [6] found a decreased oxidative capacity and an increased glycolytic capacity in quadriceps femoris muscle. MALTAIS et al.[3] reported a decreased oxidative capacity in quadriceps femoris muscle. In another recent study [4], in tibialis anterior muscle, indications for an imbalance in adenosine triphosphate (ATP) utilization and resynthesis were found, as suggested by increased inosine monophosphate (IMP) levels, which were negatively related with ATP/adenosine diphosphate (ADP) ratios. However, in that study oxidative and glycolytic enzyme capacities were not investigated.In view of the above mentioned findings in skel...

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Section: Collection and Analysis Of Muscle Biopsiesmentioning

confidence: 99%

Muscle metabolic status in patients with severe COPD with and without long-term prednisolone

Pouw¹,

Lang²,

Gosker³

et al. 2000

Eur Respir J

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“…Until recently, information about the complicated patterns of muscle glycogen balance and its relationship with exercise has only been available by using needle biopsies that could measure muscle glycogen concentrations (Harris et al 1974;Dietrichson et al 1987). While biopsies provided the original broad outline of muscle glycogen metabolism, the number and frequency of measurements and the sampling of only a small volume in a nonhomogeneous tissue were limitations of the method (Taylor et al 1992).…”

Section: Muscle Metabolism: Nmr: Glycogen: Exercise: Insulinmentioning

confidence: 99%

NMR of glycogen in exercise

1999

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Natural-abundance 13 C NMR spectroscopy is a non-invasive technique that enables in vivo assessments of muscle and/or liver glycogen concentrations. Over the last several years, 13 C NMR has been developed and used to obtain information about human glycogen metabolism with diet and exercise. Since NMR is non-invasive, more data points are available over a specified time course, dramatically improving the time resolution. This improved time resolution has enabled the documentation of subtleties of muscle glycogen re-synthesis following severe glycogen depletion that were not previously observed. Muscle and liver glycogen concentrations have been tracked in several different human populations under conditions that include: (1) muscle glycogen recovery from intense localized exercise with normal insulin and with insulin suppressed; (2) muscle glycogen recovery in an insulin-resistant population; (3) muscle glycogen depletion during prolonged low-intensity exercise; (4) effect of a mixed meal on postprandial muscle and liver glycogen synthesis. The present review focuses on basic 13 C NMR and gives results from selected studies.

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“…The CC group was also provided with a single dose of 350 mg cocoa flavanol supplementation as I.V nutrition began. Muscle biopsies of m. vastus lateralis were taken at 0, 120 and 250 min using the conchotome biopsy technique (Dietrichson et al 1987), with 1% lignocaine as local anaesthetic. The muscle tissue was washed in ice-cold saline to remove excess blood with visible fat and connective tissue removed, before being snap frozen in liquid nitrogen and stored at -80°C until further analysis (Figure 1).…”

Section: Acute Studiesmentioning

confidence: 99%