Concise review: Periodontal tissue regeneration using pericardium membrane as guided bone regeneration.

Setyawati, Ernie Maduratna; Klana, Nahdhiya Amalia Puspita

doi:10.1063/5.0036635

Cited by 2 publications

(6 citation statements)

References 22 publications

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“…The study design included the evaluation of HPLFs proliferation in contact with bovine pericardium membranes by means of XTT essay and the morphology of HPLFs in contact with bovine pericardium membranes by means of light and scanning electron microscopy, as previously described [ 12 , 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…As in previous studies [ 12 , 13 , 14 ], cells were plated in 60-mm diameter plastic culture dishes together with the X1 and X2 samples. The dishes were incubated under cell culture conditions.…”

Section: Methodsmentioning

confidence: 99%

“…As in previous studies [ 12 , 13 , 14 ], HPLFs were seeded in petri dishes containing cover glasses together with the X1 and X2 samples the tested materials. Petri dishes were seeded without membranes to serve as a negative control.…”

Section: Methodsmentioning

confidence: 99%

“…Bovine pericardium membranes showed significant functional and morphological potential, which provides the opportunity to examine cellular behavior, also in different fields [ 4 ]. Several studies in fact have successfully detected positive histological findings, due to the use of bovine pericardium membranes [ 12 ]. Furthermore, Athar et al report the potential role of lyophilized bovine pericardium, in periodontal ligament fibroblast attachment, migration, and proliferation [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Proliferation and Morphological Assessment of Human Periodontal Ligament Fibroblast towards Bovine Pericardium Membranes: An In Vitro Study

et al. 2022

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Over the past decade regenerative branches of dentistry have taken on more and more importance, resulting in the development of performing scaffold materials. These should induce cell adhesion, support, and guide the tissues’ growth. Among the developed materials, we can include resorbable or non-membranes. The purpose of this study was to investigate the proliferation abilities and the attachment of human periodontal ligament fibroblasts (HPLIFs) over two bovine pericardium membranes with different thicknesses, 0.2 mm and 0.4 mm, respectively. These membranes have been decellularized by the manufacturer, preserving the three-dimensional collagen’s structure. The HPLFs were cultured in standard conditions and exposed to the tested materials. XTT was performed to assess cell proliferation, while light microscopy (LM) and scanning electron microscopy (SEM) observations assessed fibroblast morphology at different times (T1, T2, and T3). Proliferation assays have shown a statistically significant difference in growth at T1 (p < 0.05) in the cells cultured with a thicker membrane compared to the thinner one. LM analysis showed healthy fibroblasts in contact with the membranes, appearing larger and with a polygonal shape. SEM observation demonstrated thickening of the fibroblasts which continued to adhere to the membrane’s surface, with enlarged polygonal shape and developed filipodia and lamellipodia. These results showed a similar cell behavior over the two bovine pericardium membranes, demonstrating a cellular migration along and within the layers of the membrane, binding with membrane fibers by means of filopodial extensions. Knowledge of the effects of the collagen membranes derived from bovine pericardium on cellular behavior will help clinicians choose the type of scaffolds according to the required clinical situation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proliferation and Morphological Assessment of Human Periodontal Ligament Fibroblast towards Bovine Pericardium Membranes: An In Vitro Study

et al. 2022

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“…Their aim was to investigate the viability of the pericardium membrane in restoring tooth-supporting tissues lost because of inflammatory periodontal disease. Noted for its biocompatibility and efficacy in tissue repair, the pericardium membrane demonstrated potential as an appropriate material for guided tissue regeneration (GTR) [ 4 ]. The researchers also examined a collagen membrane derived from porcine pericardium for GBR in minipigs.…”

Section: Introductionmentioning

confidence: 99%

Assessing Genotoxicity of Bovine Pericardium in Guided Tissue Regeneration: Bacterial Reverse Mutation Assay With Exogenous Metabolic Activation

Abdulrazzaq

2024

Cureus

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Concise review: Periodontal tissue regeneration using pericardium membrane as guided bone regeneration.

Cited by 2 publications

References 22 publications

Proliferation and Morphological Assessment of Human Periodontal Ligament Fibroblast towards Bovine Pericardium Membranes: An In Vitro Study

Proliferation and Morphological Assessment of Human Periodontal Ligament Fibroblast towards Bovine Pericardium Membranes: An In Vitro Study

Assessing Genotoxicity of Bovine Pericardium in Guided Tissue Regeneration: Bacterial Reverse Mutation Assay With Exogenous Metabolic Activation

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