Concomitant knockdown resistance allele, L982W + F1534C, in Aedes aegypti has the potential to impose fitness costs without selection pressure

Uemura, Nozomi; Furutani, Shogo; Tomita, Takashi; Itokawa, Kentaro; Komagata, Osamu; Kasai, Shinji

doi:10.1016/j.pestbp.2023.105422

Cited by 7 publications

(2 citation statements)

References 42 publications

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“…Experimental evolution confirmed the strong positive response of the kdr 1011Met allele to selection with a high dose of deltamethrin. Such experiment also evidenced a gradual decrease of this allele in both non-selected lines, supporting a fitness costs in absence of insecticide as previously shown for other kdr mutations (Rigby et al, 2020; Uemura et al, 2023). Indeed, the co-existence of the Met allele carried by the full length VGSC gene and the duplicated Ile allele carried by a pseudogene would not confer a fitness cost reduction as seen for the Ace-1 locus in Culex pipiens sp.…”

Section: Discussionsupporting

confidence: 86%

A genomic duplication spanning multiple P450s contributes to insecticide resistance in the dengue mosquitoAedes aegypti

Bacot,

Haberkorn,

Guilliet

et al. 2024

Preprint

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Resistance of mosquitoes to insecticides is one example of rapid adaptation to anthropogenic selection pressures having a strong impact on human health and activities. Target-site modification and increased insecticide detoxification are the two main mechanisms underlying insecticide resistance in mosquitoes. While target-sites mutations are well characterised and often used to track resistance in the field, the genomic events associated with insecticide detoxification remain partially characterised. Recent studies evidenced the key role of gene duplications in the over-expression of detoxification enzymes and their potential use to track metabolic resistance alleles in the field. However, such genomic events remain difficult to characterise due to their complex genomic architecture and their co-occurrence with other resistance alleles. In this concern, the present work investigated the role of a large genomic duplication affecting a cluster of detoxification enzymes in conferring resistance to the pyrethroid insecticide deltamethrin in the mosquito Aedes aegypti. Two isofemale lines originating from French Guiana and being deprived from major target-site mutations showed distinct insecticide resistance levels. Combining RNA-seq and whole genome pool-seq identified a 220 Kb genomic duplication spanning multiple cytochrome P450s carried by the resistant line. The genomic architecture of the duplicated loci was elucidated through long read sequencing, evidencing its transposon-mediated evolutionary origin. The importance of this P450 duplication in deltamethrin survival was supported by genotype-phenotype association and RNA interference. Experimental evolution suggested that this P450 duplication is associated with a significant fitness cost, potentially affecting its adaptive value in presence of other resistance alleles. Overall, this study supports the importance of genomic duplications affecting detoxification enzymes in the rapid adaptation of mosquitoes to insecticides. Deciphering their genomic architecture provides new insights into the evolutionary processes underlying such rapid adaptation. Such findings and provides new tools for the surveillance and management of resistance in the field.

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Section: Discussionsupporting

confidence: 86%

A genomic duplication spanning multiple P450s contributes to insecticide resistance in the dengue mosquitoAedes aegypti

Bacot,

Haberkorn,

Guilliet

et al. 2024

Preprint

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“…aegypti in the capital city, Ouagadougou [ 39 ]. Concomitant presence of these point mutations was also reported to strongly increase the level of pyrethroid resistance in individual mosquitoes [ 44 ]. In our study, high proportions of female Aedes mosquitoes displayed double or triple mutations, possibly implying a high level of pyrethroid resistance [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

First report of kdr mutations in the voltage-gated sodium channel gene in the arbovirus vector, Aedes aegypti, from Nouakchott, Mauritania

Ould Lemrabott,

Briolant,

Gomez

et al. 2023

Parasites Vectors

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Background Since 2014, dengue epidemics have occurred almost annually in Nouakchott, the capital city of Mauritania, coinciding with the recent establishment of Aedes aegypti, the primary vector of dengue, in the city. Anopheles arabiensis, the primary vector of malaria, is also abundant not only in Nouakchott but also in most areas of the country. Resistance to insecticides has been studied in An. arabiensis but not in Ae. aegypti in Mauritania. The objective of the present study was to establish the baseline data on the frequencies of knockdown resistance (kdr) mutations in the voltage-gated sodium channel (vgsc) gene in Ae. aegypti collected in Nouakchott to improve vector control. Methods Resting Ae. aegypti mosquitoes were collected in 2017 and 2018 in Teyarett and Dar Naim districts in Nouakchott using a battery-powered aspirator. Polymerase chain reaction (PCR) and DNA sequencing were performed to detect the presence of five kdr mutations known to be associated with pyrethroid resistance: L982W, S989P, I1011M/G, V1016G/I, and F1534C. Results A total of 100 female Ae. aegypti mosquitoes were identified among collected resting culicid fauna, of which 60% (60/100) were unfed, 12% (12/100) freshly blood-fed, and 28% (28/100) gravid. Among the mutations investigated in this study, 989P, 1016G, and 1534C were found to be widespread, with the frequencies of 0.43, 0.44, and 0.55, respectively. Mutations were not found in codons 982 and 1011. No other mutations were detected within the fragments analyzed in this study. Genotype distribution did not deviate from Hardy–Weinberg equilibrium. The most frequent co-occurring point mutation patterns among Ae. aegypti mosquitoes were the heterozygous individuals 989SP/1016VG/1534FC detected in 45.1% of mosquitoes. In addition, homozygous mutant 1534CC co-occurred simultaneously with homozygous wild type 989SS and 1016VV in 30.5% of mosquito specimens. Inversely, homozygous wild-type 1534FF co-occurred simultaneously with homozygous mutant 989PP and 1016GG in 19.5% of the mosquitoes. Conclusions To our knowledge, this is the first study reporting the presence of three point mutations in the vgsc gene of Ae. aegypti in Mauritania. The findings of the present study are alarming because they predict a high level of resistance to pyrethroid insecticides which are commonly used in vector control in the country. Therefore, further studies are urgently needed, in particular phenotypic characterization of insecticide resistance using the standardized test. Graphical Abstract

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Pesticide Resistance

Kasai,

Hashimoto

2024

Entomology Monographs

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Concomitant knockdown resistance allele, L982W + F1534C, in Aedes aegypti has the potential to impose fitness costs without selection pressure

Cited by 7 publications

References 42 publications

A genomic duplication spanning multiple P450s contributes to insecticide resistance in the dengue mosquitoAedes aegypti

A genomic duplication spanning multiple P450s contributes to insecticide resistance in the dengue mosquitoAedes aegypti

First report of kdr mutations in the voltage-gated sodium channel gene in the arbovirus vector, Aedes aegypti, from Nouakchott, Mauritania

Pesticide Resistance

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