2004
DOI: 10.1128/jvi.78.7.3601-3620.2004
|View full text |Cite
|
Sign up to set email alerts
|

Concurrent Expression of Latent and a Limited Number of Lytic Genes with Immune Modulation and Antiapoptotic Function by Kaposi's Sarcoma-Associated Herpesvirus Early during Infection of Primary Endothelial and Fibroblast Cells and Subsequent Decline of Lytic Gene Expression

Abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) infection of in vitro target

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

29
454
2
2

Year Published

2006
2006
2016
2016

Publication Types

Select...
7
2

Relationship

1
8

Authors

Journals

citations
Cited by 284 publications
(487 citation statements)
references
References 51 publications
29
454
2
2
Order By: Relevance
“…This is perhaps due to the multifunctional nature of PAN RNA, as PAN plays more of a regulatory role early in infection, controlling host cell gene expression. Other studies have examined gene expression patterns during primary infection (22). We have now extended these studies using highly sensitive RNA-Seq.…”
Section: Discussionmentioning
confidence: 96%
“…This is perhaps due to the multifunctional nature of PAN RNA, as PAN plays more of a regulatory role early in infection, controlling host cell gene expression. Other studies have examined gene expression patterns during primary infection (22). We have now extended these studies using highly sensitive RNA-Seq.…”
Section: Discussionmentioning
confidence: 96%
“…Measurement of KSHV Gene Expression by Real-Time Reverse Transcription (RT-PCR). Total RNA was prepared from infected or uninfected cells, using an RNeasy kit (QIAGEN) as described previously, and viral gene expression was determined (48). See SI Materials and Methods for details.…”
Section: Methodsmentioning
confidence: 99%
“…Induction of the KSHV lytic cycle in BCBL-1 cells, supernatant collection, and virus purification procedures were described previously (4). KSHV DNA was extracted and copies were quantitated by real-time DNA PCR, using primers amplifying the KSHV ORF73 gene as described previously (48). The lipophilic carbocyanine DiI-1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate, [DiIC 18 3], was used to label KSHV particles using methods described previously (12,49).…”
Section: Methodsmentioning
confidence: 99%
“…Studies on kinetic of infection of endothelial cells with HHV-8 have indicated that during the initial phase (<12 h), both lytic and latent proteins are expressed, followed by gene expression restricted to latent genes (ORF73-71) in more than 90% of the cells [13,29]. The small percentage of cells infected produce infectious virions and numerous factors that influence cell growth and survival.…”
Section: Introductionmentioning
confidence: 99%