Conditioned culture for protoplasts isolated from chrysanthemum: An efficient approach

Zhou, Jing; Wang, Bochu; Zhu, Lian

doi:10.1016/j.colsurfb.2005.07.012

Cited by 18 publications

(16 citation statements)

References 28 publications

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“…71 The plating efficiency of protoplasts is significantly increased onto an agarose obtained with CM-dilution (Lolium, Chrysanthemum). 64,72 Our results established a promoting effect of 4D-CM obtained from culture of root explants of the embryogenic hybrid. This promoting effect of was clearly observed on root explants from both chicories (Fig.…”

Section: 63mentioning

confidence: 64%

“…As we also report in our system here, promotivemitogenic factors are released in conditioned medium. 64 For example, phytosulfokine a, 21,22 cell wall remodeling enzymes, [65][66][67][68] and parietal fragments such as AGPs, [31][32][33][34][35][36][37]69 or oligogalacturonide pectic fragments have been reported. 68 The use of CM obtained from wild type carrot cell suspension favored embryo development in the thermosensitive mutant ts11 that normally produced somatic embryo arrested at the globular stage.…”

Section: 63mentioning

confidence: 99%

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Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Couillerot

Windels

Vazquez

et al. 2012

Plant Signaling & Behavior

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Somatic embryogenesis (SE) in Cichorium involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (C. endivia x C. intybus) and of different commercial chicories (C. endivia and C. intybus) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly increased by pretreatment of tissues by submersion in solutions of glycerol, abscisic acid, spermine, putrescine or of combinations of these compounds. Interestingly, the most efficient of these pretreatments also had an unexpectedly high effect on the ISE of the C. intybus cultivars. The ISE of the hybrid and of the commercial chicories were increased when explants were co-cultured with highly embryogenic chicory explants or when they were cultured in conditioned medium. These observations established that unidentified SE-promoting factors are released in the culture medium. HPLC analyses of secreted Arabino-Galactan Proteins (AGPs), which are known to stimulate SE, did not allow identifying a fraction containing differentially abundant AGP candidates. However, pointing to their role in promoting SE, we found that the hybrid had a drastically higher ISE when amino sugars and L-Proline, the putative precursors of secreted AGPs, were both added to the medium.

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Section: 63mentioning

confidence: 64%

Section: 63mentioning

confidence: 99%

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Couillerot

Windels

Vazquez

et al. 2012

Plant Signaling & Behavior

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“…Depending on the dilution, algae-conditioned medium stimulates the growth, mitotic activity and biomass production of higher plants. Conditioned media derived from algae or protoplast culture are usually diluted 2-to 5-fold (Folling et al, 1995;Zhou et al, 2005). Undiluted CMs are thought to have an adverse effect on cell propagation due to the presence of toxic waste products and/or suboptimal composition, for example depleted nitrogen (Grabski and Tukaj, 2008).…”

Section: Resultsmentioning

confidence: 99%

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

Zielińska¹,

Matusiak-Mikulin²,

Grabski³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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Conditioned medium (CM) is a general term describing media in which cells have already been cultivated for some time. Such media, usually clarified by filtration, have been used by plant biotechnologists as additives supporting the growth of cell suspensions, organs and whole plants. This study examined the effect of CM obtained from green alga Desmodesmus subspicatus on the growth and functioning of the photosynthetic apparatus of Nicotiana tabacum and Arabidopsis thaliana in culture in vitro. Plants where cultured on CM diluted 1.25-, 2-and 5-fold with MS medium. The increase in fresh and dry weight was highest in tobacco and Arabidopsis cultured on CM/2 and CM/1.25 media. Those two concentrations also increased the amount of chlorophylls in both plants tested. CM improved parameter PI (reflecting the photosynthetic "vitality" of the organism) and electron transport efficiency, and increased the fraction of active reaction centers. Analysis of chlorophyll fluorescence in vivo suggests that the improvement of these plants grown in the presence of algal CM may result from stimulation of photosynthesis. Algal CM offers a convenient, cheap, universal supplement for stimulating the growth of higher plants in vitro.K Ke ey y w wo or rd ds s: : Algal exudates, in vitro culture, growth improvement, photosynthesis.

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“…Whether the addition of activated charcoal or polyvinylpyrrolidone (PVP) is beneficial for these species, as in Eustoma grandiflorum or Vitis vinifera (Zhu et al 1997), would be worth investigating. Another possibly valuable modification would be conditioned (Chen et al 2004;Komai et al 2006) or nurse type of protoplast culture (Zhou et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants

Tomiczak

Śliwińska

Rybczyński

2016

Plant Cell Tiss Organ Cult

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The morphogenic potential of five Gentiana species of medicinal and ornamental value, i.e.G. cruciata, G. kurroo, G. lutea, G. septemfida, and G. tibetica, was compared using agarose-bead leaf mesophyll protoplast culture. Modified Murashige and Skoog medium containing 2.0 mg l -1 1-naphthaleneacetic acid and 0.1 mg l -1 thidiazuron ensured the highest cell division frequency during both protoplast and protoplast-derived cell culture or callus formation. Indirect plant regeneration mainly via the somatic embryogenesis pathway was achieved for G. kurroo and G. tibetica with the greatest efficiency occurring with MS medium supplemented with 1.0 mg l -1 kinetin, 0.5 mg l -1 gibberellic acid, and 80 mg l -1 adenine sulfate. A considerable percentage of autopolyploid and aneuploid cells was detected in callus lines obtained from protoplasts using flow cytometry. The presence of polyploid cells in calli resulted in the regeneration of 85 % polyploid plants of G. kurroo and 14 % polyploids of G. tibetica. Chromosome counting and stomatal characteristic confirmed the ploidy variation among regenerants.

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Conditioned culture for protoplasts isolated from chrysanthemum: An efficient approach

Cited by 18 publications

References 28 publications

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants

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