Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro

Abstract

Human mesenchymal stem cells (MSCs) have an intrinsic property for homing towards tumor sites and can be used as tumor-tropic vectors for tumor therapy. But very limited studies investigated the antitumor properties of MSCs themselves. In this study we investigated the antiglioma properties of two easily accessible MSCs, namely, human adipose tissue-derived mesenchymal stem cells (ASCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs). We found (1) MSC conditioned media can significantly inhibit the growth of human U251 glioma cell line; (2) MSC conditioned media can significantly induce apoptosis in human U251 cell line; (3) real-time PCR experiments showed significant upregulation of apoptotic genes of both caspase-3 and caspase-9 and significant downregulation of antiapoptotic genes such as survivin and XIAP after MSC conditioned media induction in U 251 cells; (4) furthermore, MSCs conditioned media culture induced rapid and complete differentiation in U251 cells. These results indicate MSCs can efficiently induce both apoptosis and differentiation in U251 human glioma cell line. Whereas UC-MSCs are more efficient for apoptosis induction than ASCs, their capability of differentiation induction is not distinguishable from each other. Our findings suggest MSCs themselves have favorable antitumor characteristics and should be further explored in future glioma therapy.

Human-derived normal mesenchymal stem/stromal cells in anticancer therapies

“…Co-injection of MSCs with U87MG promotes tumor growth in vivo and also induces a reduction in apoptotic cell death. AT-MSCs and UC-MSCs can efficiently induce apoptosis and differentiation in human glioma cell line U251 in vitro 41. UC-MSCs have a significant cytotoxicity against malignant glioma cell line U87MG, and mixed-source MSCs have significantly higher cytotoxicity than UC-MSCs alone for glioma cell line C6.…”

Therapeutic effects of umbilical cord blood derived mesenchymal stem cell-conditioned medium on pulmonary arterial hypertension in rats

“…21,22 CM of hUCB-MSCs contain sizable levels of angiopoietin, hepatocyte growth factor, interleukin-4, insulin-like growth factor, placental growth factor, vascular endothelial cell growth factor, angiogenin, stem cell factor, and tyrosine hydroxylase. 5,[23][24][25] Our previous studies demonstrated the neuroprotective effects of conditioned media from hADSC and hNSC in rat models of stroke and Huntington's disease.…”

Review article: mesenchymal stromal cell therapy for inflammatory bowel diseases

“…Despite their immunosuppressive properties, MSCs did not increase the risks of infection or malignancy in clinical trials . Some animal studies suggested that MSCs could promote tumour growth, or conversely have a tumour‐suppressive activity . These contradictory results could be explained by a context‐dependent role of MSCs in regulating tumour growth .…”