Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology

Cortella, Lucas R. X.; Cestari, Idágene Aparecida; Lahuerta, Ricardo Doll; Araña, M. C.; Soldera, Marcos; Rank, Andreas; Lasagni, Andrés Fabían; Cestari, Ismar N.

doi:10.1088/1748-605x/ac1f73

Cited by 5 publications

(8 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the circularity index was measured according to the previously reported method. 34 As shown in Figure 5e, the circularity index values of the hiPSC-CMs on AN and AN-MR substrates were significantly lower compared to the control, indicating that aligned nanofibers were capable of promoting cellular anisotropy. The circularity index in the AN-MR group was further lower than that in the AN group.…”

Section: Structural Maturation Of Hipsc-cmsmentioning

confidence: 85%

“…The aspect ratio of cells was defined as long axes/short axes. The circularity index of cells was defined as 4π area/perimeter 2 as previously described …”

Section: Materials and Methodsmentioning

confidence: 99%

“…The circularity index of cells was defined as 4π area/perimeter 2 as previously described. 34 2.7. Expression of hiPSC-CM Maturation-Related Genes.…”

Section: Fabrication Of An-mr Substratementioning

confidence: 99%

See 2 more Smart Citations

Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes

Tian

Liang

Lin

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Cell alignment widely exists in various in vivo tissues and also plays an essential role in the construction of in vitro models, such as vascular endothelial and myocardial models. Recently, microscale and nanoscale hierarchical topographical structures have been drawing increasing attention for engineering in vitro cell alignment. In the present study, we fabricated a micro-/nanohierarchical substrate based on soft lithography and electrospinning to assess the synergetic effect of both the aligned nanofibrous topographical guidance and the off-ground culture environment provided by the substrate on the endothelium formation and the maturation of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The morphology, proliferation, and barrier formation of human umbilical vein endothelial cells (HUVECs) as well as the alignment, cardiac-specific proteins, and maturity-related gene expression of hiPSC-CMs on the aligned-nanofiber/microridge (AN-MR) substrate were studied. Compared with the glass slide and the single-aligned nanofiber substrate, the AN-MR substrate enhanced the proliferation, alignment, and cell−cell interaction of HUVECs and improved the length of the sarcomere and maturation-related gene expression of hiPSC-CMs. Finally, the response of hiPSC-CMs on different substrates to two typical cardiac drugs (isoproterenol and E-4031) was tested and analyzed, showing that the hiPSC-CMs on AN-MR substrates were more resistant to drugs than those in other groups, which was related to the higher maturity of the cells. Overall, the proposed micro-/nanohierarchical substrate supports the in vitro endothelium formation and enhances the maturation of hiPSC-CMs, which show great potential to be applied in the construction of in vitro models and tissue engineering.

show abstract

Section: Structural Maturation Of Hipsc-cmsmentioning

confidence: 85%

“…The aspect ratio of cells was defined as long axes/short axes. The circularity index of cells was defined as 4π area/perimeter 2 as previously described …”

Section: Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes

Tian

Liang

Lin

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…The mechanical properties and in particular the stiffness of the substrate play an important in the cell fate decision of the stem cells [19][20][21][22][23][24][25][26][27][28][29][30][31][32]. Stem cells probe the substrate and regulate the intracellular contractility and stiffness [17,25].…”

Section: Discussionmentioning

confidence: 99%

“…This would suggest that hiPSCs are very sensitive to the surface morphology of the gelatin substrate and that the nanofibrous pattern would allow more efficient cell anchoring on the substrate and pluripotency maintenance of the cells [16]. Generally speaking, the properties of the substrate, including its stiffness, permeability, morphology, and chemical composition, influence to a large extent the behavior of the cells such as adhesion, proliferation, survival, and differentiation [19][20][21][22][23][24][25][26][27]. Intuitively, the nanofibrous morphology resembles some parts of in vivo ECMs, which provides more appropriate ligand accessibility and spaces underneath of the cells for diffusional transport of cytokines and large proteins [16,[29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Nanocasting of fibrous morphology on a substrate for long-term propagation of human induced pluripotent stem cells

Li¹,

Yoshioka²,

Li³

et al. 2022

Biomed. Mater.

View full text Add to dashboard Cite

Human-induced pluripotent stem cells (hiPSCs) can be self-renewed for many generations on nanofibrous substrates. Herein, a casting method is developed to replicate the nanofibrous morphology into a thin layer of polymethylsiloxane (PDMS). The template is obtained by electrospinning and chemical crosslinking of gelatin nanofibers on a glass slide. The replicas of the template are surface-functionalized by gelatin and used for propagation of hiPSCs over tenth generations. The performance of the propagated hiPSCs is checked by immunofluorescence imaging, flowcytometry, and RT-PCR, confirming the practicability of this method. The results are also compared to those obtained using electrospun nanofiber substrates. Inherently, the PDMS replica is of low stiffness and can be reproduced easily. Compared to other patterning techniques, casting is more flexible and cost-effective, suggesting that this method might find applications in cell-based assays that rely on stringent consideration of both substrate stiffness and surface morphology.

show abstract

Advancing Cardiomyocyte Maturation: Current Strategies and Promising Conductive Polymer‐Based Approaches

Elkhoury,

Kodeih,

Enciso‐Martínez

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Cardiovascular diseases are a leading cause of mortality and pose a significant burden on healthcare systems worldwide. Despite remarkable progress in medical research, the development of effective cardiovascular drugs has been hindered by high failure rates and escalating costs. One contributing factor is the limited availability of mature cardiomyocytes (CMs) for accurate disease modeling and drug screening. Human induced pluripotent stem cell‐derived CMs offer a promising source of CMs; however, their immature phenotype presents challenges in translational applications. This review focuses on the road to achieving mature CMs by summarizing the major differences between immature and mature CMs, discussing the importance of adult‐like CMs for drug discovery, highlighting the limitations of current strategies, and exploring potential solutions using electro‐mechano active polymer‐based scaffolds based on conductive polymers. However, critical considerations such as the trade‐off between three‐dimensional systems and nutrient exchange, biocompatibility, degradation, cell adhesion, longevity, and integration into wider systems must be carefully evaluated. Continued advancements in these areas will contribute to a better understanding of cardiac diseases, improved drug discovery, and the development of personalized treatment strategies for patients with cardiovascular disorders.This article is protected by copyright. All rights reserved

show abstract

Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology

Cited by 5 publications

References 51 publications

Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes

Aligned Nanofibrous Net Deposited Perpendicularly on Microridges Supports Endothelium Formation and Promotes the Structural Maturation of hiPSC-Derived Cardiomyocytes

Nanocasting of fibrous morphology on a substrate for long-term propagation of human induced pluripotent stem cells

Advancing Cardiomyocyte Maturation: Current Strategies and Promising Conductive Polymer‐Based Approaches

Contact Info

Product

Resources

About