Conditions affecting growth and developmental competence of mammalian oocytes <i>in vitro</i>

Hirao, Yuji

doi:10.1111/j.1740-0929.2010.00870.x

Cited by 24 publications

(22 citation statements)

References 105 publications

(215 reference statements)

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“…Oocyte maturation, which is essential for the subsequent fertilization and embryo development, can be easily perturbed by a series of regulatory events such as oxidative stress, apoptosis and epigenetic modifications [19]. Oxidative stress can negatively affect spindle integrity and biological function [20], lower meiotic resumption rates [21], and then decrease oocyte maturation potential.…”

Section: Introductionmentioning

confidence: 99%

Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms

et al. 2016

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Lipopolysaccharide disturbs the secretion of gonadotropin, endometrial function and implantation efficiency. However, there is little information regarding the effects of lipopolysaccharide on cyclic ovary activity, especially oocyte maturation. Therefore, we aimed to investigate the effects of lipopolysaccharide on the maturation potential of bovine oocytes. We found that lipopolysaccharide exposure significantly decreased the first polar body extrusion rate and delayed the cell cycle progression. The abnormal spindle rate was significantly increased in lipopolysaccharide treatment group, accompanied by disrupted localization and level of phosphorylated mitogen-activated protein kinase (p-MAPK). Moreover, lipopolysaccharide treatment significantly increased intracellular reactive oxygen species (ROS) levels and the early apoptotic rate in oocytes. The pro-apoptotic caspase-3 and Bax mRNA levels and caspase-3 protein level were significantly increased, whereas the anti-apoptotic Bcl-2 and XIAP transcript abundance were significantly decreased in lipopolysaccharide exposure group. Furthermore, the dimethyl-histone H3 lysine 4 (H3K4me2) level was significantly increased, while the DNA methylation (5-mC) and dimethyl-histone H3 lysine 9 (H3K9me2) levels were markedly decreased in oocytes treated with lipopolysaccharide. In conclusion, lipopolysaccharide exposure inhibits the maturation potential of bovine oocytes by affecting cell cycle, cytoskeletal dynamics, oxidative stress, and epigenetic modifications.

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Section: Introductionmentioning

confidence: 99%

Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms

et al. 2016

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“…Furthermore, we cultured OGCs derived from EAFs of young and aged cows using two types of media containing low and high concentration of estradiol; low estradiol concentration was based on the corresponding levels in follicular fluid [23]. High concentration of estradiol was based on those reported in previous reports, which yielded high antrum OGCs formation in vitro [24], because antrum formation is a primary marker of follicular development in vitro [25,26]. We also compared the developmental ability of the oocytes grown under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

Itami

Kawahara-Miki

Kawana

et al. 2014

J Assist Reprod Genet

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“…Despite the establishment of excellent culture systems in which oocytes can grow [32], a single optimal oxygen concentration has not been determined. Two mouse studies reached different conclusions, one finding that a 5% oxygen environment was more beneficial, at least in the early phase of culture [33], and the other finding that a 20% oxygen environment was better [34].…”

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confidence: 99%

Optimization of Oxygen Concentration for Growing Bovine Oocytes <i>In Vitro</i>: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

Hirao

Shimizu

Iga

et al. 2012

Journal of Reproduction and Development

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Abstract:The oxygen environment in cell culture has a significant impact on the health and performance of cells. Here, we compared the effects of reduced (5%) and ambient (20%) oxygen concentrations on bovine oocyte-granulosa cell complexes, each containing a growing oocyte 90-102 µm in diameter, cultured for 14 days. Both oxygen concentrations showed some advantages and disadvantages; in 5% oxygen, the survival rate of oocytes was significantly higher than in 20% oxygen, but the resulting oocytes were significantly smaller, which was a serious disadvantage. During the first 4 days of culture, the growth and viability of oocytes were satisfactory using 5% oxygen. This observation led us to examine the effect of changing the oxygen concentration from 5% to 20% on Day 4 in order to minimize the expected disadvantages of constant 5% and 20% oxygen. The largest population of fully grown oocytes was obtained from cultures in which the oxygen concentration was changed in this way, which also led to higher oocyte viability than in constant 20% oxygen. A similar tendency was found in the frequency of oocytes becoming blastocysts after in vitro fertilization. Surviving oocytes eventually became located within an enlarged dome-like structure, and although the 5% oxygen environment may have been appropriate for oocyte growth in the early stages, 20% oxygen may have been necessary for the growth of oocytes in the dome-like structure. These results indicate an effective way of modulating oxygen concentration according to the growth of oocyte-granulosa cell complexes in vitro. Key words: Bovine, Granulosa cell, Growing oocyte, In vitro, Oxygen concentration (J. Reprod. Dev. 58: [204][205][206][207][208][209][210][211] 2012) M ost of the growing oocytes stored in the mammalian ovary are eventually lost, and in order to be able to utilize them, it is important to develop culture systems that effectively support oocyte growth and the acquisition of developmental competence. We have previously described a 2-week culture system [1] in which bovine oocytes obtained from early antral follicles are able to complete the growth phase. However, the resulting oocytes are on average smaller than those grown in follicles in vivo. Further improvement of the culture system is therefore necessary.Our previous work comparing bovine oocyte growth in reduced (5%) and ambient (20%) oxygen concentrations [2] suggests the importance of oxygen optimization in determining culture conditions. The oxygen environment has significant effects on cell activities in culture in various cell types, such as somatic cells [3,4], embryos [5,6] and stem cells [7]. Although a universal optimal oxygen concentration for cell culture has not been determined, many studies on embryos [8][9][10], preantral follicles [11], testicular germ cells [12] and other cells [13][14][15][16][17] have suggested that a reduced oxygen concentration gives better results than an ambient oxygen concentration. A partial explanation for this would be that there is less cell damage asso...

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Conditions affecting growth and developmental competence of mammalian oocytes in vitro

Cited by 24 publications

References 105 publications

Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms

Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms

Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

Optimization of Oxygen Concentration for Growing Bovine Oocytes <i>In Vitro</i>: Constant Low and High Oxygen Concentrations Compromise the Yield of Fully Grown Oocytes

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