Conducting polymer based fluorescence quenching as a new approach to increase the selectivity of immunosensors

Ramanavičius, Arūnas; Kurilčik, N.; Juršėnas, Saulius; Finkelsteinas, A.; Ramanavičienė, Almira

doi:10.1016/j.bios.2007.06.013

Cited by 62 publications

(36 citation statements)

References 45 publications

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“…50 mM and 200 mM aniline concentrations were used for composite preparation. The lower concentration do not provided conformal coating but the higher concentration significantly quenched PL of ZnO, what is in line with similar effect observed for interaction of some other photoluminescence exhibiting materials with other conducting polymer -polypyrrole [18,19].…”

Section: Structural Properties Of Zno-pani Nanocompositessupporting

confidence: 85%

ZnO/polyaniline composite based photoluminescence sensor for the determination of acetic acid vapor

Turemis

Zappi

Giardi

et al. 2020

Talanta

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In this study, we report a novel ZnO/polyaniline (PANI) nanocomposite optical gas sensor for the determination of acetic acid at room temperatures. ZnO nanorods, synthesized in powder form were coated by PANI (ZnO/ PANI) by chemical polymerization method. The obtained nanocomposites were deposited on glass substrate and dried overnight at room temperature. Structure and optical properties of ZnO/PANI nanocomposite have been studied by using X-ray diffraction, transmission electron microscopy, scanning electron microscopy, diffuse reflectance and photoluminescence spectroscopy. Tests towards acetic acids were performed in the range of concentrations 1-13 ppm. The adsorption of acetic acid on the sensor's surface resulted in the decrease of ZnO/ PANI photoluminescence. The response and recovery time of the sensor were in the range of 30-50 s and 5 min, respectively. The developed sensors showed sensitivity towards acetic acid in a range of 1-10 ppm with the limit of detection of 1.2 ppm. Specially designed miniaturized sensing system based on integrated sensing layer, light emission diode as excitation source and optical fiber spectrometer was developed for the measurement of the sensor signal. The developed sensing system was applied for the investigation of some real sample assessment including the evaluation of storage conditions of ancient cellulose acetate films, which during the degradation are releasing acetic acid. The obtained results suggest that the developed novel optical ZnO/PANI nanocompsite based sensor shows great potential for acetic acid determination in various samples.

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Section: Structural Properties Of Zno-pani Nanocompositessupporting

confidence: 85%

ZnO/polyaniline composite based photoluminescence sensor for the determination of acetic acid vapor

Turemis

Zappi

Giardi

et al. 2020

Talanta

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“…Immunosensors make use of antigen‐antibody interactions 1, 2 to detect a wide range of analytes which are of great interest in medical diagnostics, environmental analysis, and forensic medicine 3, 4, including pathogens 5, drugs 6, bacteria 7, toxins 8, and biomarkers 9. Overall, immunosensors employ the same chemical approach of earliest immunoassays, but offer quicker and simpler analytical procedures that may be conducted at the point‐of‐care 10.…”

Section: Introductionmentioning

confidence: 99%

Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label‐Free Human Chorionic Gonadotropin Detection

et al. 2014

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Abstract:A new immunosensor is presented for human against the logarithm-hCG concentration for 0.1-25 ng/ chorionic gonadotropin (hCG), made by electrodepositmL with limit of detection of 0.016 ng/mL. High selectivi-ing chitosan/goldnanoparticles over graphene screenty was observed in blank urine and successful detection printed electrode (SPE). The antibody was covalently of hCG was also achieved in spiked samples of real urine bound to CS via its Fc-terminal. The assembly was confrom pregnant woman. The immunosensor showed good trolled by electrochemical Impedance Spectroscopy (EIS) detection capability, simplicity of fabrication, low-cost, and followed by Fourier Transformed Infrared (FTIR). high sensitivity and selectivity. The hCG-immunosensor displayed linear response

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“…However, Ramanvicius et al [27] used Ppy fluorescence quenching to develop an immunoassay against bovine leukemia virus protein gp51. They attributed the quenching to the proximity of the Cy5 to the delocalized π-π electrons in the Ppy backbone, as described by Song et al [28].…”

Section: Resultsmentioning

confidence: 99%

Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

Maurer

Yazvenko

Wilmoth

et al. 2010

Sensors

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The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized polypyrrole (Ppy). Hybridization was measured using a biotinylated target oligonucleotide and either Cy5-streptavidin and fluorescence detection or horseradish peroxidase-streptavidin and enzyme-enhanced electrochemical detection. Detection efficiencies were optimized by varying the deposition of the Ppy, the terminal groups on the DNA probes, and other factors that impacted fluorescence quenching and electrical conductivity. Optimized results were compared against those obtained using a microarray with the same DNA sequences synthesized in situ. Immobilized probes produced higher fluorescence signals, possibly by providing a greater stand off between the Cy5 on the target oligonucleotide and the quenching effects of the Ppy and the platinum electrode.

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Conducting polymer based fluorescence quenching as a new approach to increase the selectivity of immunosensors

Cited by 62 publications

References 45 publications

ZnO/polyaniline composite based photoluminescence sensor for the determination of acetic acid vapor

ZnO/polyaniline composite based photoluminescence sensor for the determination of acetic acid vapor

Chitosan/AuNPs Modified Graphene Electrochemical Sensor for Label‐Free Human Chorionic Gonadotropin Detection

Use of a Multiplexed CMOS Microarray to Optimize and Compare Oligonucleotide Binding to DNA Probes Synthesized or Immobilized on Individual Electrodes

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