Condurango 30C Induces Epigenetic Modification of Lung Cancer-specific Tumour Suppressor Genes via Demethylation

Abstract

Background: DNA hypermethylation induces cancer progression involving CpG island of DNA and causes inactivation of tumour suppressor genes. In this study, DNA hypermethylation status of lung cancer and ability of ultra-highly diluted Condurango 30C to modulate DNA methylation were ascertained by analysis of lung cancer-specific tumour suppressor genes in respect to placebo. Materials and Methods: DNA methylation status, if any, was determined by PCR-SSCP analyses in lung cancer-specific tumour suppressor genes (p15, p16 and p53) using H460-NSCLC cell and BaP-induced lung cancer of rats. The ability of Condurango 30C to modulate DNA methylation, if any, was verified against placebo control in blinded manner. Results: Condurango 30C-treated DNA showed significant decrease in band intensity of p15 and p53 genes especially in methylated condition in vitro, at IC₅₀ dose (2.43µl/100µl). SSCP analysis of p15 and p53 genes in Condurango 30C-treated DNA also suggests that Condurango 30C can decrease methylation, in vitro. Inhibition of p15 hypermethylation was observed in post-cancer treatment of rats with Condurango 30C. SSCP results gave a better indication of differences in band position of p15 and p53 in Condurango 30C-treated lung samples. Conclusion: Condurango 30C could trigger epigenetic modification in lung cancer via modulation of DNA hypermethylation.

Effects of Homeopathic Phosphorus on Encephalitozoon cuniculi-Infected Macrophages In-Vitro

“…Cellular mechanisms related to the effects of homeopathic treatments have been well described in recent years and are related to the epigenetic control of specific genes, which depends on the nature of the medicine used and its dilution. 17,18,29,[32][33][34][35] Based on our previous experience, macrophages respond to specific homeopathic stimuli by expressing specific cytokines and chemokines depending on the experimental context. 13,15 The choice of Phos to be used in this study was based on the classical homeopathic similia principle, since the main symptoms of encephalito-zoonosis are listed in Phos materia medica and previous clinical observations reinforced the utility of this medicine for this kind of epidemiological problem.…”

Polyperspektivität in der Medizin: Dilemma oder Chance? Zur Ganzheitlichkeit der Homöopathie Hahnemanns

“…Kritische Sichtweisen reichen etwa von dem Einwand, dass Hochpotenzen rein rechnerisch ultramolekulare Verdünnungen seien, weshalb Homöopathie eine Scheintherapie sein müsse, bis zur selektiven Interpretation ausgewählter klinischer Studien im Rahmen der Standards einer «evidence-based medicine» oder der neodogmatischen Ansicht, die Homöopathie erfülle nicht die Voraussetzungen einer «science-based medicine» [10,11]. Der Homöopathie günstige Perspektiven kommen unter anderem aus der Grundlagenforschung, etwa durch neuere Forschungsergebnisse hinsichtlich Nanopartikel [12][13][14][15], Hormesis [16,17], Genexpressivität [18,19], Epigenetik [20] usw. [21,22].…”

Die Erforschung molekularer Aspekte homöopathischer Arzneiwirkungen

“…Epigenetische Modifikationen Sie sind das Markenzeichen von Krebs. Kürzlich wurden experimentelle Untersuchungen von uns durchgeführt [5,23,24,29], um zu untersuchen, ob bestimmte potenzierte homöopathische Mittel die Fähigkeit besitzen, epigenetische Modifikationen bei einigen Krebszellen in vivo und in vitro umzukehren. Die Ergebnisse der Pilotstudie zur DNA-Methylierung/Demethylierung ermutigen sehr zu der Annahme, dass bestimmte ultra-hoch verdünnte homöopathische Arzneimittel die DNA-Methylierung/Demethylierung zur Regulation von Genexpressionen vorteilhaft modulieren können.…”