Confirmation and Determination of Sugars in Soft Drink Products by IEC with ESI-MS

Determination of sucrose in equine serum using liquid chromatography–mass spectrometry (LC/MS)

“…The column was operated at 30 • C with a flow rate of 0.5 mL/min. Sample injection was 20 L and achieved by use of an autosampler maintained at 4 • C. Conditions for the mobile phases were modified from previous publications [4,11] as follows: A -aqueous solution containing 0.1% acetic acid; B -acetonitrile containing 0.1% acetic acid. Elution began with 90:10 (A:B) for 2 min, increasing linearly to 0:100 over 8 min, isocratic for 3 min at 0:100, decreasing linearly to 90:10 over 3 min and maintained isocratically at 90:10 for 3 min, for a total run time of 17 min.…”

“…for GC/MS methods for detection of polar constituents [9,11]. Conversion of target compounds to volatile derivatives for analysis and quantitation using GC/MS is time consuming and potentially damaging to heat labile compounds [11].…”

“…Conversion of target compounds to volatile derivatives for analysis and quantitation using GC/MS is time consuming and potentially damaging to heat labile compounds [11]. Other problems include excess labelling and subsequent problems with separation or mass spectrometry techniques, as well as derivative reagents reacting with target compounds [12].…”

HPLC mapping of second generation ethanol production with lignocelluloses wastes and diluted sulfuric hydrolysis

“…It is highly relevant to emphasize that several researchers, such as Chen et al (2009Chen et al ( , 2010 , using Balfourodendron riedelianum. The reminiscent presence of this carbohydrate indicates that fructose was consumed at a lower rate than glucose during the initial hours of the fermentation assay.…”

The Modern Art of Identification of Natural Substances in Whole Plants

“…Thus NPLC, contrary to APPI, leads to poor results when it is directly coupled to ESI. By contrast, although salts and ion-pairing agents may cause ion suppression, ion-exchange chromatography (Chen et al , 2009 and ion-pairing reversed-phase liquid chromatography de Person et al 2008) remain sufficiently compatible with ESI-MS. RPLC and HILIC are the most universal LC modes for hyphenation. The only circumspection is to avoid too concentrated buffers for pH or ionic strength control.…”