Confocal optics microscopy for biochemical and cellular high-throughput screening

Translation of a Tumor Microenvironment Mimicking 3D Tumor Growth Co-culture Assay Platform to High-Content Screening

“…For automated fluorescent image acquisition, the Opera QEHS (PerkinElmer, Waltham, MA), [15][16][17] the MIAS-2 (Digilab, Holliston, MA), 18 or the IN Cell Analyzer 2000 (GE Healthcare Life Sciences, Little Chalfont, UK) 19 was used. The MIAS-2 and IN Cell 2000 can also acquire brightfield images.…”

“…The PerkinElmer Opera QEHS [15][16][17] is a microlensenhanced Nipkow spinning disk confocal system for fluorescence imaging. It is powered by four lasers and images of three colors can be acquired in parallel by the three cameras.…”

High-Throughput Screening with HyperCyt® Flow Cytometry to Detect Small Molecule Formylpeptide Receptor Ligands

“…FIDA is also most typically used for probing cell lysates because the small interrogation volume is more suitable for measuring ligand interactions with membrane vesicles or small diameter (<200 nm) beads. 14,15 LSI is in essence an implementation of FIDA in which the excitation laser is continuously scanned through the sample volume during the measurement period. This results in an extended series of confocal volume measurements that comprises a fluorescence image.…”

High-Content Screening: A Decade of Evolution

“…These include the development of large and complex high-throughput instruments such as the Nipkow disk laser confocal Opera, the laser line scanning confocal IN Cell Analyzer 3000, and the laser scanning cytometry Explorer. 4,5 However, the main thrust of instrument development has been in flexible bench-top instruments serving a wide user base. From the development of the Cellomics ArrayScan in the late 1990s, these instruments, together with their associated software and reagent wetware, have provided the platforms on which the majority of HCS applications are run today.…”