Confocal Raman study of aging process in diabetes mellitus human voluntaries

Abstract

Accumulation of AGEs [Advanced Glycation End -products] occurs slowly during the human aging process. However, its formation is accelerated in the presence of diabetes mellitus. In this paper, we perform a noninvasive analysis of glycation effect on human skin by in vivo confocal Raman spectroscopy. This technique uses a laser of 785 nm as excitation source and, by the inelastic scattering of light, it is possible to obtain information about the biochemical composition of the skin. Our aim in this work was to characterize the aging process resulting from the glycation process in a group of 10 Health Elderly Women (HEW) and 10 Diabetic Elderly Women (DEW). The Raman data were collected from the dermis at a depth of 70-130 microns. Through the theory of functional density (DFT) the bands positions of hydroxyproline, proline and AGEs (pentosidine and glucosepane) were calculated by using Gaussian 0.9 software. A molecular interpretation of changes in type I collagen was performed by the changes in the vibrational modes of the proline (P) and hydroxyproline (HP). The data analysis shows that the aging effects caused by glycation of proteins degrades type I collagen differently and leads to accelerated aging process.

In vivo Confocal Raman Spectroscopic Analysis of the Effects of Infrared Radiation in the Human Skin Dermis

“…The redshift observed at 1664 cm −1 for T60 in group II indicates the change in (Amide I) α ‐helical structure . The observed shift and the intensity differences for various bio‐molecules may be due to the conformational changes because of IR radiation.…”

Noninvasive assessments of skin glycated proteins by fluorescence and Raman techniques in diabetics and nondiabetics

“…An inelastic scattering of monochromatic laser photons by matter which lead to energy transfer and molecular vibrations is elucidated by the Raman phenomenon, promoting a molecular fingerprint . Currently, the studies conducted by Pereira et al showed the effect of chronological aging and the glycation process on the human dermis in diabetic patients and, young and old healthy people using confocal Raman spectroscopy.…”