Conically Shaped Chromatographic Column

Abstract

We investigated a column with varying physical properties. A change in velocity was induced by a continuously varying the diameter inside the column. Analytical dimensions of the column were used. The diameter was continuously reduced from the analytical one (4.0 mm) to a diameter typical for narrow-bore columns (2.5 mm). Our interest was focused on the efficiency of a conical column and on the mobile phase velocity in it. The results obtained with the conical column were compared to those obtained with the conventional analytical column and the narrow column. The diameter of the narrow column (3.3 mm) was the same as the average diameter of the conical column. The advantage of the conical column over the narrow column was the greater loadability of the conical column and better dynamic efficiency. Compared to the conventional analytical column, a higher linear velocity at the same flow rate was achieved. Therefore, the retention times were shorter and consumption of the mobile phase was smaller.

Study on conical columns with different conical angles for semi-preparative liquid chromatography

“…[3] showed higher column efficiency because of less wall effect, but has the drawback of lower sample capacity than cylindrical column, waste of solvents and packing materials. Pecavar et al [4] reported a conically shaped analytical column (150 mm × 4 mm i.d. → 2.5 mm i.d., conical angle 0.6 • ).…”

Study on conical columns for semi‐preparative liquid chromatography

“…A conical column with a smaller inlet diameter compared with its outlet [3] showed a higher column efficiency because of a reduced wall effect, but a smaller sample capacity than a cylindrical column. Pecavar et al [4] reported a conically shaped analytical column (150 mm64 mm ID 2.5 mm ID). Kerry invented a 25 mm62 mm 0.5 mm conical column [5].…”