Conifer Biotechnology: An Overview

Rodríguez, Sonia María; Ordás, Ricardo J.; Álvarez, José Marcos Alonso

doi:10.3390/f13071061

Cited by 7 publications

(1 citation statement)

References 189 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytokinins play several recognized roles in plant development, through the suppression of apical dominance and promotion of the development of axillary buds, the promotion of cell division, or the stimulation of plant protein synthesis [16,36]. BA is the most commonly used plant growth regulator; it is applied alone or in combination with other CK to promote in vitro shoot induction due to its effectiveness and affordability [37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

View full text Add to dashboard Cite

Cryptomeria japonica (Thunb. ex L.f.) D. Don is a commercial tree native to Japan and is one of the most important forest species in that country and the Azores (Portugal). Because of the quality of C. japonica timber, several genetic improvement programs have been performed. Recently, some studies focusing on C. japonica somatic embryogenesis have been carried out. However, in this species, this process uses immature seeds as initial explants, and for this reason, it is not possible to achieve the maximum genetic gain (100% genetic of the donor plant). Although some studies have been made applying organogenesis to this species, the success of the process in adult trees is low. For this reason, our main goal was to optimize the micropropagation method by using trees older than 30 years as a source of plant material. In this sense, in a first experiment, we studied the effect of different types of initial explants and three basal culture media on shoot induction; then, two sucrose concentrations and two light treatments (LEDs versus fluorescent lights) were tested for the improvement of rooting. In a second experiment, the effects of different plant growth regulators (6-benzylaminopurine, meta-topolin, and thidiazuron) on shoot induction and the subsequent phases of the organogenesis process were analyzed. The cultures produced the highest number of shoots when QL medium (Quoirin and Lepoivre, 1977) and long basal explants (>1.5 cm) were used; the shoots obtained produced a higher number of roots when they were grown under red LED lights. Moreover, root induction was significantly higher in shoots previously induced with meta-topolin.

show abstract

Section: Discussionmentioning

confidence: 99%

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

View full text Add to dashboard Cite

show abstract

Genome Editing in Forest Trees

Bruegmann,

Fendel,

Zahn

et al. 2023

A Roadmap for Plant Genome Editing

View full text Add to dashboard Cite

Since the first CRISPR/Cas-mediated genome editing of poplar in 2015, an increasing number of tree species are being genome-edited. Although the availability of genome sequences, tissue culture and transformation systems are limiting factors, research is ongoing on advanced methods such as DNA-free genome editing and gene targeting approaches in addition to the optimisation of single gene knockouts. These can be used to address ambitious issues and perform genome editing more accurately, which has implications for the legal assessment of edited trees. Once technically established, CRISPR/Cas can be used to circumvent specific challenges related to forest tree species, e.g., longevity and extended vegetative phases, and to modify traits relevant for breeding, whether for direct application or to elucidate the genetic basis of individual traits. Not least due to climate change, adaptation to abiotic stress such as drought stress as well as biotic stresses caused by pathogens are strongly in focus. For the use as a renewable resource and as a carbon sink, wood productivity in forest trees as well as wood properties are of interest. In biosafety assessments, tree-specific aspects have to be considered, which result, among other aspects, from the long lifespan.

show abstract