Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar

Tsuyama, Taku; Takabe, Keiji

doi:10.1007/s10086-015-1486-7

Cited by 16 publications

(27 citation statements)

References 26 publications

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“…First, compound 1D is found in the cambial sap of many tree species [1,5]. Second, coniferin β-glucosidase is located in the cell wall of coniferous and broad-leaf trees [6,7]. Third, the incorporation of radioactivity into cell wall lignin is observed when radiolabeled compound 1D is fed to a variety of plants [8].…”

Section: Introductionmentioning

confidence: 99%

Preparation and properties of a coniferin enantiomer

et al. 2019

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l-Coniferin (1L), which is an enantiomer of natural coniferin (d-coniferin (1D)), was prepared from l-glucose according to the conventional method for compound 1D. The reactivity of l-glucose and its derivatives was found to be almost same as that of the corresponding d-glucose and its derivatives during the preparation for compound 1L. Compound 1L showed resistance toward enzymatic hydrolysis by commercial β-glucosidase from Almond. However, unlike compound 1D, compound 1L was not transported across the membrane obtained from differentiating xylem of a hybrid poplar in the present assay. The result suggested for the first time that the d-/l-configuration of the glucose moiety of coniferin is an important factor affecting coniferin transport across the membrane.

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Section: Introductionmentioning

confidence: 99%

Preparation and properties of a coniferin enantiomer

et al. 2019

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“…This programmed monolignol delivery is reasonably understood by programmed supply of MLGs (Terashima et al 1993(Terashima et al , 1998 combined with localization of specific β-D-glucosidase for specific MLGs in the differentiating xylem. Coniferin specific β-glucosidase has been found in the secondary cell wall of pine (Dharmawardhana et al 1995;Samuels et al 2002) and poplar (Tsuyama and Takabe 2015).…”

Section: Role Of Mlgs In Lignification Of Cell Wallsmentioning

confidence: 99%

“…Low levels of the enzyme activity were detectable in the bryophytes and pteridophytes, while highly specific activities of the enzyme were found in most of the woody angiosperms and almost all gymnosperm species (Ibrahim 1977). Wide distribution of β-glucosidase specific for MLGs (Marcinowski and Grisebach 1978;Marcinowski et al 1979;Samuels et al 2002;Chapelle et al 2012;Tsuyama and Takabe 2015) suggests that glucosidation and deglucosidation steps are essential for the formation of macromolecular lignin in both gymnosperm and angiosperm xylem cell walls. However, the size of the intermediate pool differs among plant species.…”

Section: Pool Size Of Mlgs As Intermediates In Lignin Biosynthesismentioning

confidence: 99%

“…The MLGs are considered to be storage and transport form of the monolignols, and the glucosidation and deglucosidation steps may regulate lignin biosynthesis, while the former is mediated by a uridine diphosphate glucose (UDP-glucose): monolignol glucosyltransferase and the latter is induced by 4-O-β-D-glucosidase (Ibrahim and Grisebach 1976;Ibrahim 1977;Terazawa et al 1984;Dharmawardhana et al 1995;Dharmawardhana and Ellis 1998;Steeves et al 2001;Samuels et al 2002;Lanot et al 2006;Tsuyama and Takabe 2015).…”

Section: Introductionmentioning

confidence: 99%

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Monolignol glucosides as intermediate compounds in lignin biosynthesis. Revisiting the cell wall lignification and new ¹³C-tracer experiments with Ginkgo biloba and Magnolia liliiflora

Terashima¹,

Matsushita

et al. 2016

Holzforschung

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Monolignol glucosides as intermediate compounds in lignin biosynthesis. Revisiting the cell wall lignification and new 13C-tracer experiments with Ginkgo biloba and Magnolia liliiflora DOI 10.1515/hf-2015-0224 Received October 17, 2015 accepted February 9, 2016; previously published online March 14, 2016 Abstract: A large amount of monolignol glucosides (MLGs: p-glucocoumaryl alcohol, coniferin, syringin) are found in lignifying soft xylem near cambium and they disappear with the progress of lignification. Recently, it became a matter of debate whether those MLGs are real intermediates in lignin biosynthesis or only a storage form of monolignols outside of the main biosynthetic pathway. The latter is partly based on a misinterpretation of 14 C-tracer experiments and partly on the simple generalization of the results of gene manipulation experiments concerning the flexible and complex lignification. In the present paper, it could be confirmed by the most reliable 13 C-tracer method that MLGs are real intermediates in the pathway from l-phenylalanine to macromolecular ligninpolysaccharides complexes in the cell walls. This pathway via MLGs is essential for transport and programmed delivery of specific monolignols in a stable form from intracellular space to specific lignifying sites within the cell wall. The pool size of MLGs is large in most gymnosperm trees and some angiosperm species that emerged in an early stage of phylogeny, while the pool size is small in most angiosperms. This difference in pool size is reasonably understandable from the viewpoint of plant evolution, in the course of which the role of MLGs changed to meet variation in type of major cells, reaction wood formation, and postmortem lignification.

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“…At1g61810 (AtBGLU45), At1g61820 (AtBGLU46) and At4g21760 (AtBGLU47) in Arabidopsis play an important role in the synthesis of Arabidopsis lignin [16,17]. Os4BGLU14, Os4BGLU16 and Os4BGLU18 found in the rice β-glucosidase family have been proved to be functional genes in rice lignin synthesis [18].In addition, a β-glucosidase was identi ed from the poplar tree and named 'PtrBGLU6', which plays an important role in poplar ligni cation and cell wall formation [19].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

Wang

Yang

Han

et al. 2020

Preprint

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Background: The β-glucosidase BGLU in the glycoside hydrolase family 1 (GH1) is involved in the sugar metabolism of the plant and plays an important role in maintaining the normal physiological function of the plant. Recent studies had shown that β-glucosidase was involved in plant lignification. The lignification in pear fruit is closely related to the formation of pear stone cells, but the BGLU genes family has not been identified in pears.Result: A total of 343 BGLU genes were identified from five species of Rosaceae (Pyrus bretschneideri, Prunus mume, Malus domestica, Prunus avium, Fragaria vesca). According to phylogenetic analysis, 50 PbBGLUs were divided into 8 groups. 298 syntenic pairs were found in intra- and inter-species collinear analysis of five Rosaceae species, found that pears and apples had more syntenic pairs than pear and the other three Rosaceae species. The Ka/Ks analysis of duplication PbBGLU genes in pear indicated that the main mode of expansion of the PbBGLUs was segmental replication and was mainly affected by purification. qRT-PCR showed that the three gene expression patterns of PbBGLU1, PbBGLU15 and PbBGLU16 were basically consistent with the change trend of pear fruit lignin and stone cell content, and may be involved in lignification and stone cell development of pear fruit. Subcellular localization showed that these three candidate genes were all located on the cell wall.Conclusion: In this study, a genome-wide analysis of BGLU genes in five Rosaceae species was carried out, and three candidate genes related to lignification and stone cell development of pear fruits were identified, which laid the foundation for a deeper understanding of the function of BGLU genes in pear fruits and potential in changing pear fruit quality.

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Coniferin β-glucosidase is ionically bound to cell wall in differentiating xylem of poplar

Cited by 16 publications

References 26 publications

Preparation and properties of a coniferin enantiomer

Preparation and properties of a coniferin enantiomer

Monolignol glucosides as intermediate compounds in lignin biosynthesis. Revisiting the cell wall lignification and new ¹³C-tracer experiments with Ginkgo biloba and Magnolia liliiflora

Genome-Wide Comparative Analysis of the β-glucosidase Family in Five Rosaceae Species and their Potential Role on Lignification of Stone Cells in Chinese White Pear

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