Conjugation Ratio, Light Dose, and pH Affect the Stability of Panitumumab–IR700 for Near-Infrared Photoimmunotherapy

Fujimura, Daiki; Inagaki, Fuyuki; Okada, Ryuhei; Rosenberg, Adrian; Furusawa, Aki; Choyke, Peter L.; Kobayashi, Hisataka

doi:10.1021/acsmedchemlett.0c00262

Cited by 13 publications

(12 citation statements)

References 34 publications

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“…Phthalocyanine compounds have a strong absorption capacity in the NIR region and are promising photosensitizers that can be used for fluorescence imaging and PDT tumor therapy. 18 Hisataka Kobayashi’s team, 19 , 20 in their search for a new near-infrared (NIR) fluorescent group, discovered a dye called IRDye700DX, 21 a water-soluble silico Rn phthalocyanine derivative that, when coupled with antibodies, was highly lethal only to target cells irradiated in near-infrared, with almost no off-target toxicity. IRDye700DX (IR700), a photoactivated substance that binds antibodies, was activated by 690-nm near-infrared light to induce cell killing.…”

Section: Photoimmunoconjugatementioning

confidence: 99%

Photoimmunotherapy: A New Paradigm in Solid Tumor Immunotherapy

Peng

Huang

2022

Cancer Control

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In recent years, the incidence of cancer has been increasing worldwide. Conventional cancer treatments include surgery, chemotherapy, and radiation, which mostly kill tumor cells at the expense of normal and immune cells. Although immunotherapy is an accurate, rapid, efficient tumor immune treatment, it causes serious adverse reactions, such as cytokine release syndrome (CRS) and neurotoxicity. Therefore, there is an urgent need to develop an effective and nontoxic procedure for immunotherapy. The clinical combination of phototherapy and immunoadjuvant therapy can induce immunogenic cell death and enhance antigen presentation synergy. It also causes a systemic antitumor immune response to manage residual tumors and distant metastases. Photoimmunotherapy (PIT) is a tumor treatment combining phototherapy with immunotherapy based on injecting a conjugate photosensitizer (IR700) and a monoclonal antibody (mAb) to target an expressed antigen on the tumor surface. This combination can enhance the immune response ability, thus having a good effect on the treatment of residual tumor and metastatic cancer. In this review, we summarize the recent progress in photoimmunotherapy, including photoimmunoconjugate (PIC), the activation mechanism of immunogenic cell death (ICD), the combination therapy model, opportunities and prospects. Specifically, we aim to provide a promising clinical therapy for solid tumor clinical transformation.

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Section: Photoimmunoconjugatementioning

confidence: 99%

Photoimmunotherapy: A New Paradigm in Solid Tumor Immunotherapy

Peng

Huang

2022

Cancer Control

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“…Conjugation of dye with monoclonal antibody was performed as previously described. 12 Detailed materials and methods are described in Data S1.…”

Section: Materials S and Me Thodsmentioning

confidence: 99%

Diagnostic imaging in near‐infrared photoimmunotherapy using a commercially available camera for indocyanine green

et al. 2021

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Near‐infrared photoimmunotherapy (NIR‐PIT) is a new type of cancer treatment, which was recently approved in Japan for patients with inoperable head and neck cancer. NIR‐PIT utilizes antibody‐IRDye700DX (IR700) conjugates and NIR light at a wavelength of 690 nm. NIR light exposure leads to physicochemical changes in the antibody‐IR700 conjugate cell receptor complex, inducing rapid necrotic cell death. Just as fluorescence guided surgery is useful for surgeons to resect tumors completely, real‐time information of tumor locations would help clinicians irradiate NIR light more precisely. IR700 is a fluorescence dye that emits at 702 nm; however, there is no clinically available device optimized for detecting this fluorescence. On the other hand, many indocyanine green (ICG) fluorescence imaging devices have been approved for clinical use. Therefore, we investigated whether LIGHTVISION, one of the clinically available ICG cameras, could be employed for tumor detection. We hypothesized that irradiation with even low‐power 690‐nm laser light, attenuated by 99% with a neutral‐density filter, could be detected with LIGHTVISION without fluorescence decay or therapeutic effect because of the long emission tail of IR700 beyond 800 nm (within the detection range of LIGHTVISION). We demonstrated that the LIGHTVISION camera, originally designed for ICG detection, can detect the tail of IR700 fluorescence in real time, thus enabling the visualization of target tumors.

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“…Furthermore, a photo-immunotherapy using new photosensitizer, IR700 (excited at 690 nm), has been reported as a principle similar to the CALI method. 82 , 83 ) Because these excitation wavelengths are still short, these are currently limited to the tissue surface, such as skin cancer. Therefore, the development of new photosensitizers that can be excited at longer wavelengths is needed in the future.…”

Section: Future Innovations In Calimentioning

confidence: 99%

Optical manipulation of molecular function by chromophore-assisted light inactivation

Takemoto

2021

Proc. Jpn. Acad., Ser. B

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In addition to simple on/off switches for molecular activity, spatiotemporal dynamics are also thought to be important for the regulation of cellular function. However, their physiological significance and in vivo importance remain largely unknown. Fluorescence imaging technology is a powerful technique that can reveal the spatiotemporal dynamics of molecular activity. In addition, because imaging detects the correlations between molecular activity and biological phenomena, the technique of molecular manipulation is also important to analyze causal relationships. Recent advances in optical manipulation techniques that artificially perturb molecules and cells via light can address this issue to elucidate the causality between manipulated target and its physiological function. The use of light enables the manipulation of molecular activity in microspaces, such as organelles and nerve spines. In this review, we describe the chromophore-assisted light inactivation method, which is an optical manipulation technique that has been attracting attention in recent years.

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Conjugation Ratio, Light Dose, and pH Affect the Stability of Panitumumab–IR700 for Near-Infrared Photoimmunotherapy

Cited by 13 publications

References 34 publications

Photoimmunotherapy: A New Paradigm in Solid Tumor Immunotherapy

Photoimmunotherapy: A New Paradigm in Solid Tumor Immunotherapy

Diagnostic imaging in near‐infrared photoimmunotherapy using a commercially available camera for indocyanine green

Optical manipulation of molecular function by chromophore-assisted light inactivation

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