Conjugative Coupling Proteins and the Role of Their Domains in Conjugation, Secondary Structure and in vivo Subcellular Location

Álvarez-Rodríguez, Itxaso; Ugarte‐Uribe, Begoña; Arada, Igor de la; Arrondo, José Luis R.; Garbisu, Carlos; Alkorta, Itziar

doi:10.3389/fmolb.2020.00185

Cited by 10 publications

(6 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three ATPases (VirD4, VirB4, and VirB11) comprise the cytoplasmic energy center, which is situated at the base of the translocation channel. Of these ATPases, VirD4 serves as the receptor to which both DNA and protein substrates bind prior to entry into the translocation channel (Alvarez‐Rodriguez et al., 2020b; Llosa and Alkorta, 2017). The channel itself consists of two large subassemblies, one spanning the inner membrane (IM) and the second situated in the periplasm and outer membrane (OM) (Christie et al., 2005; Low et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Rather, the two TSs map to the C‐proximal helicase domain, specifically, within the 2B/2B‐like subdomains of an SF1A/B helicase structural fold (Ilangovan et al., 2017). These TSs likely contribute to binding of the relaxosome to cognate VirD4 receptors, also termed type IV coupling proteins (T4CPs) (Alvarez‐Rodriguez et al., 2020b; Llosa and Alkorta, 2017), but details of these interactions are not yet available.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Type IV secretion systems: Advances in structure, function, and activation

Costa

Harb

Khara

et al. 2021

Molecular Microbiology

162

152

View full text Add to dashboard Cite

Many bacterial species deploy Type IV Secretion Systems (T4SSs) to deliver DNA, protein, or other macromolecules to bacterial or eukaryotic cell targets (Li et al., 2019;Waksman, 2019). The T4SSs are composed mainly of two subfamilies, the conjugation systems and effector translocators (Cascales and Christie, 2003). Conjugation systems are of considerable medical concern for their roles in dissemination of mobile genetic elements (MGEs), often encoding resistance to heavy metals or antibiotics (Cabezon et al., 2015;Huddleston, 2014;Koraimann, 2018). The effector translocators mainly deliver proteins to eukaryotic target cells, although recent studies have also documented the interkingdom transfer of DNA,

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Type IV secretion systems: Advances in structure, function, and activation

Costa

Harb

Khara

et al. 2021

Molecular Microbiology

162

152

View full text Add to dashboard Cite

show abstract

“…The TraI encoded by the IncPα plasmid RP4 ( Pansegrau et al., 1993 ) is regarded as a representative of MOB P family. T4CPs are essential elements in conjugative T4SSs and are also key elements in many pathogenic T4SSs ( Álvarez-Rodríguez et al., 2020 ). In this study, all the 66 bla OXA-181 -positive plasmids in Enterobacterales carried the genes encoding T4CPs of the VirD4/TraG subfamily characterized by the domain “T4SS-DNA_transf (Pfam: PF02534)”.…”

Section: Discussionmentioning

confidence: 99%

In silico characterization of IncX3 plasmids carrying blaOXA-181 in Enterobacterales

Zhang

Shi

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Carbapenem-resistant Enterobacterales poses a global urgent antibiotic resistance threat because of its ability to transfer carbapenemase genes to other bacteria via horizontal gene transfer mediated by mobile genetic elements such as plasmids. Oxacillinase-181 (OXA-181) is one of the most common OXA-48-like carbapenemases, and OXA-181-producing Enterobacterales has been reported in many countries worldwide. However, systematic research concerning the overall picture of plasmids harboring blaOXA-181 in Enterobacterales is currently scarce. In this study, we aimed to determine the phylogeny and evolution of blaOXA-181-positive (gene encoding OXA-181) plasmids. To characterize the plasmids harboring blaOXA-181 in Enterobacterales, we identified 81 blaOXA-181-positive plasmids from 35,150 bacterial plasmids downloaded from the NCBI RefSeq database. Our results indicated that diverse plasmid types harbored blaOXA-181 but was predominantly carried by IncX3-type plasmids. We systematically compared the host strains, plasmid types, conjugative transfer regions, and genetic contexts of blaOXA-181 among the 66 blaOXA-181-positive IncX3 plasmids. We found that IncX3 plasmids harboring blaOXA-181 were mostly ColKP3-IncX3 hybrid plasmids with a length of 51 kb each and were mainly distributed in Escherichia coli and Klebsiella pneumoniae. Most of the IncX3 plasmids harboring blaOXA-181 were human origin. Almost all the blaOXA-181-positive IncX3 plasmids were found to carry genes coding for relaxases of the MOBP family and VirB-like type IV secretion system (T4SS) gene clusters, and all the 66 IncX3 plasmids were found to carry the genes encoding type IV coupling proteins (T4CPs) of the VirD4/TraG subfamily. Most IncX3 plasmids harbored both blaOXA-181 and qnrS1 in their genomes, and the two antibiotic resistance genes were found to a composite transposon bracketed by two copies of insertion sequence IS26 in the same orientation. Our findings provide important insights into the phylogeny and evolution of blaOXA-181-positive IncX3 plasmids and further address their role in acquiring and spreading blaOXA-181 genes in Enterobacterales.

show abstract

“…The relaxases R1162, RSF1010, and R3200B, which were independently isolated but essentially identical plasmids, were the prototypes of the MOB Q family ( 28 , 29 ). T4CPs are essential elements in conjugative T4SSs and are also key elements in many pathogenic T4SSs ( 30 ). In this study, all the bla NDM -positive conjugative plasmids of Acinetobacter species were found to carry the genes encoding T4CPs of the TrwB/TraD subfamily, with the TrwB of plasmid R388 from E. coli as a representative ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

In Silico Characterization of bla _NDM -Harboring Conjugative Plasmids in Acinetobacter Species

et al. 2022

View full text Add to dashboard Cite

show abstract

Conjugative Coupling Proteins and the Role of Their Domains in Conjugation, Secondary Structure and in vivo Subcellular Location

Cited by 10 publications

References 58 publications

Type IV secretion systems: Advances in structure, function, and activation

Type IV secretion systems: Advances in structure, function, and activation

In silico characterization of IncX3 plasmids carrying blaOXA-181 in Enterobacterales

In Silico Characterization of bla _NDM -Harboring Conjugative Plasmids in Acinetobacter Species

Contact Info

Product

Resources

About

Conjugative Coupling Proteins and the Role of Their Domains in Conjugation, Secondary Structure and in vivo Subcellular Location

Cited by 10 publications

References 58 publications

Type IV secretion systems: Advances in structure, function, and activation

Type IV secretion systems: Advances in structure, function, and activation

In silico characterization of IncX3 plasmids carrying blaOXA-181 in Enterobacterales

In Silico Characterization of bla NDM -Harboring Conjugative Plasmids in Acinetobacter Species

Contact Info

Product

Resources

About

In Silico Characterization of bla _NDM -Harboring Conjugative Plasmids in Acinetobacter Species