Conjugative plasmid transfer betweenPseudomonas strains within alginate bead microcosms: Effect of the internal gel structure

Mater, Denis D.G.; Saucedo, J. E. Nava; Truffaut, Nicole; Barbotin, Jean‐Noël; Thomas, Daniel

doi:10.1002/(sici)1097-0290(19991005)65:1<34::aid-bit5>3.0.co;2-z

Cited by 5 publications

References 22 publications

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Arsenite oxidation in batch reactors with alginate‐immobilized ULPAs1 strain

Simeonova¹,

Micheva²,

Müller³

et al. 2005

Biotech & Bioengineering

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Arsenic is one of the major groundwater contaminants worldwide. It was previously demonstrated that the beta-proteobacterium Cenibacterium arsenoxidans has an efficient As[III] oxidation ability. The present study was conducted to evaluate the performance of alginate-immobilized ULPAs1 in the oxidation of As[III] to As[V] in batch reactors. A two-level full factorial experimental design was applied to investigate the influence of main parameters involved in the oxidation process, i.e., pH (7-8), temperature (4 degrees C-25 degrees C), kind of nutrient media (2%-20% sauerkraut brine), and arsenic concentration (10-100 mg/L). One hundred milligram per liter of As[III] was fully oxidized by calcium-alginate immobilized cells in 1 h. It was found that the temperature as well as the kind of nutrient media used were significant parameters at a 95% confidence interval whereas only temperature was a significant parameter at a 99% confidence interval. The immobilization of the As[III] oxidizing strain in alginate beads offers a promising way to implement new treatment processes in the remediation of arsenic contaminated waters.

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