Connection between biomechanics and cytoskeleton structure of lymphocyte and Jurkat cells: An AFM study

Cai, Xuan; Xing, Xiaoyan; Cai, Jun; Chen, Qian; Wu, Shi-Xian; Huang, Feicheng

doi:10.1016/j.micron.2009.08.011

Cited by 76 publications

(64 citation statements)

References 56 publications

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“…A decreased F-actin content or disruption of the actin cytoskeleton with cytochalasin D have been linked to decreased elastic moduli (Cai et al, 2010;Janmey et al, 1991;Kasas et al, 2005). Adrenergic stimulation of osteoblasts led to a decreased F-to G-actin ratio compared to untreated cells.…”

Section: Discussionmentioning

confidence: 99%

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

Lee-Thedieck

Rauch

Fiammengo

et al. 2012

Journal of Cell Science

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SummaryIn the bone marrow, hematopoietic stem cells (HSCs) reside in endosteal and vascular niches. The interactions with the niches are essential for the maintenance of HSC number and properties. Although the molecular nature of these interactions is well understood, little is known about the role of physical parameters such as matrix elasticity. Osteoblasts, the major cellular component of the endosteal HSC niche, flatten during HSC mobilization. We show that this process is accompanied by osteoblast stiffening, demonstrating that not only biochemical signals but also mechanical properties of the niche are modulated. HSCs react to stiffer substrates with increased cell adhesion and migration, which could facilitate the exit of HSCs from the niche. These results indicate that matrix elasticity is an important factor in regulating the retention of HSCs in the endosteal niche and should be considered in attempts to propagate HSCs in vitro for clinical applications.

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Section: Discussionmentioning

confidence: 99%

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

Lee-Thedieck

Rauch

Fiammengo

et al. 2012

Journal of Cell Science

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“…The cell cytoskeleton is responsible for the determination of both cell shape and mechanical properties [11,13]. Every cell line has a specific shape and size which hold a specialized function.…”

Section: Discussionmentioning

confidence: 99%

Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties

Liu

Zheng

et al. 2015

Biochemical and Biophysical Research Communications

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“…According to parallel researches, made at atomic-force and confocal laser scanning microscopes, a strong connection was detected between lowering of Young's modulus in blood cells in persons with leucosis and content of Factin, concentration of which lowers in cells twice in comparison with normal lymphocytes [4]. Reduction of thickness and content of actin-like structures in lymphocytes of patients with CLL is reflected at peculiarities of morphology and charge of transformed cells' surface.…”

Section: серия физиология Phisiology Seriesmentioning

confidence: 99%

“…4 [8]. It was shown that in lymphocytes of patients with CLL the number of β 2 -adrenoceptors is lowered, which leads to reduction of adenylate cyclase's stimulation [9].…”

mentioning

confidence: 99%

Structure and Properties of Lymphocytes’ Surfaces in Patients With Chronic Lymphoblastic Leucosis

Тикунова¹,

Belyaeva²,

Cherkashina³

et al. 2015

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In the investigation that was performed, the authors studied the structure and properties of lymphocytes' surfaces in patients with chronic lymphoblastic leukemia with application of atomic force microscopy technology. It was stated that development of chronic lymphoblastic leukemia is characterized with circulation of immature forms of lymphocytic series in peripheral bloodstream, for which increase of surface potential by 456% (p<0.05) as compared with control group, is typical. Surface relief of abnormal cells is characterized by abundance of globular structures with reduced height, which endows it with relief view. Stiffness of lymphocytes in patients with lymphoblastic leukemia is reduced by 51.4% (р<0.05) as compared with control group. Revealed peculiarities of morphological and functional properties of abnormal cell clones lead to microcirculatory embarrassment in vessels, which should be considered at providing standard treatment schemes.

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Connection between biomechanics and cytoskeleton structure of lymphocyte and Jurkat cells: An AFM study

Cited by 76 publications

References 56 publications

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

Impact of substrate elasticity on human hematopoietic stem and progenitor cell adhesion and motility

Anti-pulmonary fibrotic activity of salvianolic acid B was screened by a novel method based on the cyto-biophysical properties

Structure and Properties of Lymphocytes’ Surfaces in Patients With Chronic Lymphoblastic Leucosis

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