Connection between cuticular hydrocarbons and melanization in Harmonia axyridis revealed by RNAi-mediated silencing of the CYP4G79

Zhang, Yi; Feng, Zhu-Jun; Chen, Zhan-Sheng; Wang, Xingxing; Cong, Haosu; Fan, Yongliang; Liu, Tong‐Xian

doi:10.1127/entomologia/2020/0970

Cited by 11 publications

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“…These results indicate that the decrease and increase of chitin content, or the occurrence of any obstacle in the chitin synthesis and degradation system, can cause the chitin metabolism process of insects and lead to molting and development difficulties (Li et al, 2022). Although the regulation mechanism may be different, it also gives scientists the idea to find suitable target genes from them and develop potential chitinase, chitin synthase, trehalase and trehalose-6-phosphate synthase inhibitors are used for pest control (Zhang J. Y. et al, 2021;Wang et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…Its main functions include: first, it plays an important role in the growth and development of insects, especially in the process of periodic molting (Arakane and Muthukrishanan, 2010); Second, as the main component of the body surface or trachea epidermis, it protects the internal organs of insects and prevents the invasion of foreign toxins and pathogens to organisms (Hegedus et al, 2009;Doucet and Retnakaran, 2012). Based on the important role of chitin in insect growth and development, and the absence of chitin in higher animals, the control of chitin synthesis and degradation has become a safe target for the development of new pesticides (Oyeleye and Normi, 2018;Zhang X. et al, 2021;Zhang J. Y. et al, 2021;Wang et al, 2022). In addition, the purified chitinase has certain insecticidal activity (Suganthi et al, 2017), which has attracted more and more attention.…”

Section: Introductionmentioning

confidence: 99%

“…If chitinase is continuously expressed and acts, the synthesis of chitin may still be affected after the formation of new epidermis, thus causing the development of insects to be blocked and even death (Xi et al, 2015;Liu et al, 2017). Many studies have found that once the expression of chitinase gene was inhibited, the development of insect larvae such as molting, pupation, adult eclosion and wings would be affected (Zhu et al, 2008b;Pesch et al, 2016;Dong et al, 2020;Zhang X. et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Smith), as a major migratory pest newly invading China, has expanded the area of damage and rapidly become a frequent important pest to corn ( Wu et al, 2021 ; Guo et al, 2022 ). RNAi technology has been more and more widely used in the study of insect gene function ( Zhang et al, 2021 ). Recently, with the occurrence of the harm of S. frugiperda , more and more attention has been paid to screening and studying the target genes of control by using RNAi technology ( Ghosh et al, 2016 ; Dhandapani et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Chitinase (CHI) of Spodoptera frugiperda affects molting development by regulating the metabolism of chitin and trehalose

et al. 2022

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Chitin is the main component of insect exoskeleton and midgut peritrophic membrane. Insect molting is the result of the balance and coordination of chitin synthesis and degradation in chitin metabolism under the action of hormones. In this study, a 678 bp dsRNA fragment was designed and synthesized according to the known CHI (Chitinase) sequence of Spodoptera frugiperda. It was injected into the larvae to observe the molting and development of S. frugiperda. At the same time, the activities of trehalase and chitinase, the contents of trehalose, chitin and other substances were detected, and the expression of related genes in the chitin synthesis pathway was determined. The results showed that CHI gene was highly expressed at the end of each instar, prepupa and pupal stage before molting; At 12 and 24 h after dsRNA injection of CHI gene of S. frugiperda, the expression of CHI gene decreased significantly, and the chitinase activity decreased significantly from 12 to 48 h. The expression of chitin synthase (CHSB) gene decreased significantly, and the chitin content increased significantly. Some larvae could not molt normally and complete development, leading to certain mortality. Secondly, after RNAi of CHI gene, the content of glucose and glycogen increased first and then decreased, while the content of trehalose decreased significantly or showed a downward trend. The activities of the two types of trehalase and the expression levels of trehalase genes decreased first and then increased, especially the trehalase activities increased significantly at 48 h after dsCHI injection. And trehalose-6-phosphate synthase (TPS), glutamine: fructose-6-phosphate amidotransferase (GFAT), UDP-N-acetylglucosamine pyrophosphorylases (UAP), hexokinase (HK), glucose-6-phosphate isomerase (G6PI) and phosphoacetylglucosamine mutase (PAGM) all decreased significantly at 24 h, and then increased or significantly increased at 48 h. These results indicated that when the expression of chitinase gene of S. frugiperda was inhibited, it affected the degradation of chitin in the old epidermis and the formation of new epidermis, and the content of chitin increased, which led to the failure of larvae to molt normally. Moreover, the chitin synthesis pathway and trehalose metabolism were also regulated. The relevant results provide a theoretical basis for screening target genes and developing green insecticides to control pests by using the chitin metabolism pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chitinase (CHI) of Spodoptera frugiperda affects molting development by regulating the metabolism of chitin and trehalose

et al. 2022

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“…37 The technique has significant advantages for utilization in pest control, including high specificity and efficiency that results in targeted reduction in transcripts of interest by an endogenous cellular mechanism. [38][39][40][41] There are several methods for delivering the dsRNAs that trigger RNAi, however, there are advantages and disadvantage to each approach. Microinjection, the most widely used delivery method, was first used to knockdown curl transcripts in Drosophila embryos.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of five developmental signaling network genes in the white‐backed planthopper: Potential application for pest management

Liu

Guo

Long

et al. 2023

Pest Management Science

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BACKGROUND The white‐backed planthopper (WBPH, Sogatella furcifera) is a major rice pest that exhibits condition dependent wing dimorphisms – a macropterous (long wing) form and a brachypterous (short wing) form. Although, the gene cascade that regulates wing development and dimorphic differentiation has been largely defined, the utility of these genes as targets for pest control has yet to be fully explored. RESULTS Five genes typically associated with the developmental signaling network, armadillo (arm), apterous A (apA), scalloped (sd), dachs (d), and yorkie (yki) were identified from the WBPH genome and their roles in wing development assessed following RNA interference (RNAi)‐mediated knockdown. At 5 days‐post injection, transcript levels for all five targets were substantially decreased compared with the dsGFP control group. Among the treatment groups, those injected with dsSfarm had the most pronounced effects on transcript reduction, mortality (95 ± 3%), and incidence (45 ± 3%) of wing deformities, whereas those injected with dsSfyki had the lowest incidence (6.7 ± 4%). To assess the utility of topical RNAi for Sfarm, we used a spray‐based approach that complexed a large‐scale, bacteria‐based double‐stranded RNA (dsRNA) expression pipeline with star polycation (SPc) nanoparticles. Rice seedlings infested with third and fourth instar nymphs were sprayed with SPc–dsRNA formulations and RNAi phenotypic effects were assessed over time. At 2 days post‐spray, Sfarm transcript levels decreased by 86 ± 9.5% compared with dsGFP groups, and the subsequent incidences of mortality and wing defects were elevated in the treatment group. CONCLUSIONS This study characterized five genes in the WBPH developmental signaling cascade, assessed their impact on survival and wing development via RNAi, and developed a nanoparticle‐dsRNA spray approach for potential field control of WBPH. © 2023 Society of Chemical Industry.

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RNAi-mediated CrebA silencing inhibits reproduction and immunity in Locusta migratoria manilensis

Tang¹,

Hu²,

Wang³

et al. 2023

Developmental & Comparative Immunology

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Connection between cuticular hydrocarbons and melanization in Harmonia axyridis revealed by RNAi-mediated silencing of the CYP4G79

Cited by 11 publications

References 0 publications

Chitinase (CHI) of Spodoptera frugiperda affects molting development by regulating the metabolism of chitin and trehalose

Chitinase (CHI) of Spodoptera frugiperda affects molting development by regulating the metabolism of chitin and trehalose

Functional characterization of five developmental signaling network genes in the white‐backed planthopper: Potential application for pest management

RNAi-mediated CrebA silencing inhibits reproduction and immunity in Locusta migratoria manilensis

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